β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis

The plant hormone salicylic acid (SA) is required for defense responses. NON EXPRESSER OF PATHOGENESIS RELATED 1 (NPR1) and NON RECOGNITION OF BTH-4 (NRB4) are required for the response to SA in Arabidopsis (Arabidopsis thaliana). Here, we isolated several interactors of NRB4 using yeast two-hybrid...

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Autores principales: Medina Puche, Laura, Castelló, María José, Canet, Juan Vicente, Lamilla, Julián, Colombo, María Laura, Tornero, Pablo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Arabidopsis thaliana, yeast, point mutation, protein interaction, phenotypes
Plantas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/77030
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-77030
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Arabidopsis thaliana, yeast, point mutation, protein interaction, phenotypes
Plantas
spellingShingle Ciencias Exactas
Arabidopsis thaliana, yeast, point mutation, protein interaction, phenotypes
Plantas
Medina Puche, Laura
Castelló, María José
Canet, Juan Vicente
Lamilla, Julián
Colombo, María Laura
Tornero, Pablo
β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis
topic_facet Ciencias Exactas
Arabidopsis thaliana, yeast, point mutation, protein interaction, phenotypes
Plantas
description The plant hormone salicylic acid (SA) is required for defense responses. NON EXPRESSER OF PATHOGENESIS RELATED 1 (NPR1) and NON RECOGNITION OF BTH-4 (NRB4) are required for the response to SA in Arabidopsis (Arabidopsis thaliana). Here, we isolated several interactors of NRB4 using yeast two-hybrid assays. Two of these interactors, βCA1 and βCA2, are β-carbonic anhydrase family proteins. Since double mutant βca1 βca2 plants did not show any obvious phenotype, we investigated other βCAs and found that NRB4 also interacts with βCA3 and βCA4. Moreover, several βCAs interacted with NPR1 in yeast, including one that interacted in a SA-dependent manner. This interaction was abolished in loss-of-function alleles of NPR1. Interactions between βCAs and both NRB4 and NPR1 were also detected in planta, with evidence for a triple interaction, NRB4- βCA1-NPR1. The quintuple mutant βca1 βca2 βca3 βca4 βca6 showed partial insensitivity to SA. These findings suggest that one of the functions of carbonic anhydrases is to modulate the perception of SA in plants.
format Articulo
Articulo
author Medina Puche, Laura
Castelló, María José
Canet, Juan Vicente
Lamilla, Julián
Colombo, María Laura
Tornero, Pablo
author_facet Medina Puche, Laura
Castelló, María José
Canet, Juan Vicente
Lamilla, Julián
Colombo, María Laura
Tornero, Pablo
author_sort Medina Puche, Laura
title β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis
title_short β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis
title_full β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis
title_fullStr β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis
title_full_unstemmed β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis
title_sort β-carbonic anhydrases play a role in salicylic acid perception in arabidopsis
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/77030
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AT castellomariajose bcarbonicanhydrasesplayaroleinsalicylicacidperceptioninarabidopsis
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bdutipo_str Repositorios
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