Effects of commonly used solubilizing agents on a model nerve-muscle synapse
Solubility represents a limiting factor when testing new compounds in animal experiments, since solubilizing agents generally have pharmacological effects that can interfere with the studied substance. Vehicles are commonly used for solubilizing certain substances including apolar and polar extrac...
Guardado en:
| Autores principales: | , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2008
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/7687 http://www.latamjpharm.org/trabajos/27/5/LAJOP_27_5_1_12_O2RJ9LDZPF.pdf |
| Aporte de: |
| id |
I19-R120-10915-7687 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia Medicina medicinal plant extract; neuromuscular junction; solubility Extractos de plantas Acciones farmacológicas Experimentación animal |
| spellingShingle |
Farmacia Medicina medicinal plant extract; neuromuscular junction; solubility Extractos de plantas Acciones farmacológicas Experimentación animal Cintra-Francischinelli, Mariana Silva, Magali G. Andréo Filho, Newton Cintra, Adélia C.O. Leite, Gildo B. Cruz Höfling, Maria A. da Rodrigues-Simioni, Léa Oshima-Franco, Yoko Effects of commonly used solubilizing agents on a model nerve-muscle synapse |
| topic_facet |
Farmacia Medicina medicinal plant extract; neuromuscular junction; solubility Extractos de plantas Acciones farmacológicas Experimentación animal |
| description |
Solubility represents a limiting factor when testing new compounds in animal experiments, since
solubilizing agents generally have pharmacological effects that can interfere with the studied substance. Vehicles
are commonly used for solubilizing certain substances including apolar and polar extracts obtained from medicinal
plants. In this study, fifteen vehicles were investigated on mice neuromuscular preparations. A known in vitro
neuroblocker myotoxin from Bothrops jararacussu venom, bothropstoxin-I, was used as a pharmacological tool
for testing the medicinal potential of apolar and polar extracts (hexane, dichloromethane, ethyl acetate and
methanol) obtained from Casearia sylvestris Sw. leaves, which in turn were used for testing their solubility and
concomitantly to produce no change on basal response of indirectly stimulated mouse phrenic nerve-diaphragm
preparations. Taken together in vitro biological system and extracts solubility, our results showed that dimethyl
sulphoxide and polyethylene glycol 400 were the better vehicles, and methanol extract solubilized on PEG 400
was the only one able to act against the paralysis induced by the myotoxin. Thus, this study points out to the relevant
role that vehicles exhibit for extracting the potential pharmacological activity of plants in a given test system. |
| format |
Articulo Articulo |
| author |
Cintra-Francischinelli, Mariana Silva, Magali G. Andréo Filho, Newton Cintra, Adélia C.O. Leite, Gildo B. Cruz Höfling, Maria A. da Rodrigues-Simioni, Léa Oshima-Franco, Yoko |
| author_facet |
Cintra-Francischinelli, Mariana Silva, Magali G. Andréo Filho, Newton Cintra, Adélia C.O. Leite, Gildo B. Cruz Höfling, Maria A. da Rodrigues-Simioni, Léa Oshima-Franco, Yoko |
| author_sort |
Cintra-Francischinelli, Mariana |
| title |
Effects of commonly used solubilizing agents on a model nerve-muscle synapse |
| title_short |
Effects of commonly used solubilizing agents on a model nerve-muscle synapse |
| title_full |
Effects of commonly used solubilizing agents on a model nerve-muscle synapse |
| title_fullStr |
Effects of commonly used solubilizing agents on a model nerve-muscle synapse |
| title_full_unstemmed |
Effects of commonly used solubilizing agents on a model nerve-muscle synapse |
| title_sort |
effects of commonly used solubilizing agents on a model nerve-muscle synapse |
| publishDate |
2008 |
| url |
http://sedici.unlp.edu.ar/handle/10915/7687 http://www.latamjpharm.org/trabajos/27/5/LAJOP_27_5_1_12_O2RJ9LDZPF.pdf |
| work_keys_str_mv |
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Repositorios |
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1764820484760797187 |