Osteogenic activity of vanadyl(IV)-ascorbate complex: evaluation of its mechanism of action

We have previously shown that different vanadium(IV) complexes regulate osteoblastic growth. Since vanadium compounds are accumulated in vivo in bone, they may affect bone turnover. The development of vanadium complexes with different ligands could be an alternative strategy of use in skeletal tissu...

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Autores principales: Cortizo, Ana María, Molinuevo, María Silvina, Barrio, Daniel Alejandro, Bruzzone, Liliana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Exactas
Osteogenesis; Vanadium; Proliferation; Differentiation; Protein phosphorylation
Química
Vanadio
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/76266
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-76266
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Osteogenesis; Vanadium; Proliferation; Differentiation; Protein phosphorylation
Química
Vanadio
spellingShingle Ciencias Exactas
Osteogenesis; Vanadium; Proliferation; Differentiation; Protein phosphorylation
Química
Vanadio
Cortizo, Ana María
Molinuevo, María Silvina
Barrio, Daniel Alejandro
Bruzzone, Liliana
Osteogenic activity of vanadyl(IV)-ascorbate complex: evaluation of its mechanism of action
topic_facet Ciencias Exactas
Osteogenesis; Vanadium; Proliferation; Differentiation; Protein phosphorylation
Química
Vanadio
description We have previously shown that different vanadium(IV) complexes regulate osteoblastic growth. Since vanadium compounds are accumulated in vivo in bone, they may affect bone turnover. The development of vanadium complexes with different ligands could be an alternative strategy of use in skeletal tissue engineering. In this study, we have investigated the osteogenic properties of a vanadyl(IV)–ascorbate (VOAsc) complex, as well as its possible mechanisms of action, on two osteoblastic cell lines in culture. VOAsc (2.5–25 M) significantly stimulated osteoblastic proliferation (113–125% basal, p < 0.01) in UMR106 cells, but not in the MC3T3E1 cell line. VOAsc (5–100 M) dose-dependently stimulated type-I collagen production (107–156% basal) in osteoblasts. After 3 weeks of culture, 5–25 M VOAsc increased the formation of nodules of mineralization in MC3T3E1 cells (7.7–20-fold control, p < 0.001). VOAsc (50–100 M) significantly stimulated apoptosis in both cell lines (170–230% basal, p < 0.02–0.002), but did not affect reactive oxygen species production. The complex inhibited alkaline and neutral phosphatases from osteoblastic extracts with semi-maximal effect at 10 M doses. VOAsc induced the activation and redistribution of P-ERK in a time- and dose-dependent manner. Inhibitors of the mitogen activated protein kinases (MAPK) pathway (PD98059 and UO126) partially blocked the VOAsc-enhanced osteoblastic proliferation and collagen production. In addition, wortmanin, a PI-3-K inhibitor and type-L channel blocker nifedipine also partially abrogated these effects of VOAsc on osteoblasts. Our in vitro results suggest that this vanadyl(IV)–ascorbate complex could be a useful pharmacological tool for bone tissue regeneration.
format Articulo
Articulo
author Cortizo, Ana María
Molinuevo, María Silvina
Barrio, Daniel Alejandro
Bruzzone, Liliana
author_facet Cortizo, Ana María
Molinuevo, María Silvina
Barrio, Daniel Alejandro
Bruzzone, Liliana
author_sort Cortizo, Ana María
title Osteogenic activity of vanadyl(IV)-ascorbate complex: evaluation of its mechanism of action
title_short Osteogenic activity of vanadyl(IV)-ascorbate complex: evaluation of its mechanism of action
title_full Osteogenic activity of vanadyl(IV)-ascorbate complex: evaluation of its mechanism of action
title_fullStr Osteogenic activity of vanadyl(IV)-ascorbate complex: evaluation of its mechanism of action
title_full_unstemmed Osteogenic activity of vanadyl(IV)-ascorbate complex: evaluation of its mechanism of action
title_sort osteogenic activity of vanadyl(iv)-ascorbate complex: evaluation of its mechanism of action
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/76266
work_keys_str_mv AT cortizoanamaria osteogenicactivityofvanadylivascorbatecomplexevaluationofitsmechanismofaction
AT molinuevomariasilvina osteogenicactivityofvanadylivascorbatecomplexevaluationofitsmechanismofaction
AT barriodanielalejandro osteogenicactivityofvanadylivascorbatecomplexevaluationofitsmechanismofaction
AT bruzzoneliliana osteogenicactivityofvanadylivascorbatecomplexevaluationofitsmechanismofaction
bdutipo_str Repositorios
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