Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture

An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106...

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Autores principales: Cortizo, Ana María, Sedlinsky, Claudia, McCarthy, Antonio Desmond, Blanco, Alcira, Schurman, León
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Exactas
Metformin; Osteoblast; Osteoporosis; Diabetes mellitus
Metformina
Osteoblastos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/76260
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-76260
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Metformin; Osteoblast; Osteoporosis; Diabetes mellitus
Metformina
Osteoblastos
spellingShingle Ciencias Exactas
Metformin; Osteoblast; Osteoporosis; Diabetes mellitus
Metformina
Osteoblastos
Cortizo, Ana María
Sedlinsky, Claudia
McCarthy, Antonio Desmond
Blanco, Alcira
Schurman, León
Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
topic_facet Ciencias Exactas
Metformin; Osteoblast; Osteoporosis; Diabetes mellitus
Metformina
Osteoblastos
description An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.
format Articulo
Articulo
author Cortizo, Ana María
Sedlinsky, Claudia
McCarthy, Antonio Desmond
Blanco, Alcira
Schurman, León
author_facet Cortizo, Ana María
Sedlinsky, Claudia
McCarthy, Antonio Desmond
Blanco, Alcira
Schurman, León
author_sort Cortizo, Ana María
title Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_short Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_full Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_fullStr Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_full_unstemmed Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_sort osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/76260
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AT mccarthyantoniodesmond osteogenicactionsoftheantidiabeticdrugmetforminonosteoblastsinculture
AT blancoalcira osteogenicactionsoftheantidiabeticdrugmetforminonosteoblastsinculture
AT schurmanleon osteogenicactionsoftheantidiabeticdrugmetforminonosteoblastsinculture
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