Infectivity by nematode-bacteria association on the potato weevil <i>Phyrdenus muriceus</i>

Bacteria of the genus Photorhabdus (Enterobacteriaceae) are motile, gram-negative, symbiotically associated with juveniles of entomopathogenic nematodes from the genus Heterorhabditis (Heterorhabditidae). Nematodes retain the Photorhabdus bacterial cells intheir intestine and transport them into an...

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Detalles Bibliográficos
Autores principales: Eliceche, Daiana Pamela, Rusconi, José Matías, Rosales, Matías N., Salas, Augusto, Achinelly, María Fernanda
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Naturales
Parasitología
potato weevil
nematodes
crops
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/74382
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-74382
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Parasitología
potato weevil
nematodes
crops
spellingShingle Ciencias Naturales
Parasitología
potato weevil
nematodes
crops
Eliceche, Daiana Pamela
Rusconi, José Matías
Rosales, Matías N.
Salas, Augusto
Achinelly, María Fernanda
Infectivity by nematode-bacteria association on the potato weevil <i>Phyrdenus muriceus</i>
topic_facet Ciencias Naturales
Parasitología
potato weevil
nematodes
crops
description Bacteria of the genus Photorhabdus (Enterobacteriaceae) are motile, gram-negative, symbiotically associated with juveniles of entomopathogenic nematodes from the genus Heterorhabditis (Heterorhabditidae). Nematodes retain the Photorhabdus bacterial cells intheir intestine and transport them into an insect host. Upon locating an insect host,Heterorhabditis penetrates through natural openings (mouth, anus, spiracles), or directlyinto the hemocoel via the integument, subsequently releasing bacteria into the hemolymph. Once in the hemolymph, Photorhabdus begins multiplying simultaneously releasing toxinsvirulent enough to kill the insect within 24 hrs. Phyrdenus muriceus (Coleoptera: Curculionidae) is a pest of cultivated solanaceae affecting egg-plant, potato and tomato. Considerable damages on these crops are produced by this pest affecting both the aerial andunderground parts of the plants. Infectivity of the entomopathogenic complex Heterorhabditis bacteriophora and the symbiotic bacterium Photorhabdus luminescens isolated from soil samples collected from an orchard of La Plata was tested against pupae of P. muriceus. This stage was considered to be a suitable target to control at field due to its permanence into the soil. Plastic containers (8.5 cm diameter x 5.5 cm height) were used with sterile soil forming a 2 cm high layer. Pupae were added to the soil surface and exposed to a concentration of 5.000 infective juveniles (IJs) / insect. Mortality of 70% (n=10) occurred at 48 hrs after infection. In figure 1, we can appreciate nematodes inside the body of the pupae (B and C). The cadavers showed the typical red wine coloration due to the release of the endosymbiotic bacteria in the hemocoel (Fig. 1 B-D) unlike non-parasitized pupae (Fig. 1A). The complex nematode-bacteria was effective to parasite and kill Phyrdenus muriceus pupae. Future studies should be carried out to determine its use as biological control for this horticultural pest.
format Articulo
Preprint
author Eliceche, Daiana Pamela
Rusconi, José Matías
Rosales, Matías N.
Salas, Augusto
Achinelly, María Fernanda
author_facet Eliceche, Daiana Pamela
Rusconi, José Matías
Rosales, Matías N.
Salas, Augusto
Achinelly, María Fernanda
author_sort Eliceche, Daiana Pamela
title Infectivity by nematode-bacteria association on the potato weevil <i>Phyrdenus muriceus</i>
title_short Infectivity by nematode-bacteria association on the potato weevil <i>Phyrdenus muriceus</i>
title_full Infectivity by nematode-bacteria association on the potato weevil <i>Phyrdenus muriceus</i>
title_fullStr Infectivity by nematode-bacteria association on the potato weevil <i>Phyrdenus muriceus</i>
title_full_unstemmed Infectivity by nematode-bacteria association on the potato weevil <i>Phyrdenus muriceus</i>
title_sort infectivity by nematode-bacteria association on the potato weevil <i>phyrdenus muriceus</i>
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/74382
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