Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells

The receptor mediated activation of phospholipase A2by appropriate ligands results in the synthesis and release of eicosanoids, a class of potent bioregulatory molecules. Madin-Darby canine kidney cells (MDCK) are polarized epithelial cells, with structurally and functionally distinct plasma membran...

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Detalles Bibliográficos
Autores principales: Cortizo, Ana María, Besterman, J. M., Leitner, P. P., Chandrabose, K. A.
Formato: Articulo
Lenguaje:Inglés
Publicado: 1992
Materias:
Ciencias Médicas
phospholipase A2, eicosanoids, Madin-Darby canine kidney cells
Riñón
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/67401
https://www.sciencedirect.com/science/article/pii/009069809290008H
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-67401
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
phospholipase A2, eicosanoids, Madin-Darby canine kidney cells
Riñón
spellingShingle Ciencias Médicas
phospholipase A2, eicosanoids, Madin-Darby canine kidney cells
Riñón
Cortizo, Ana María
Besterman, J. M.
Leitner, P. P.
Chandrabose, K. A.
Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
topic_facet Ciencias Médicas
phospholipase A2, eicosanoids, Madin-Darby canine kidney cells
Riñón
description The receptor mediated activation of phospholipase A2by appropriate ligands results in the synthesis and release of eicosanoids, a class of potent bioregulatory molecules. Madin-Darby canine kidney cells (MDCK) are polarized epithelial cells, with structurally and functionally distinct plasma membrane domains separated by tight junctions. Using MDCK cells grown in dual sided chambers, we show in this report, that a) the receptor mediated release of prostaglandins and arachidonate into the extracellular medium is predominantly unidirectional, b) the direction of release is agonist specific, and c) the magnitude of the response due to a given agonist is cell-domain specific. These characteristics, if operativein vivo, would contribute towards the optimal function of trans-cellular metabolism of eicosanoids already demonstrated.
format Articulo
Articulo
author Cortizo, Ana María
Besterman, J. M.
Leitner, P. P.
Chandrabose, K. A.
author_facet Cortizo, Ana María
Besterman, J. M.
Leitner, P. P.
Chandrabose, K. A.
author_sort Cortizo, Ana María
title Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
title_short Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
title_full Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
title_fullStr Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
title_full_unstemmed Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
title_sort stimulated release of arachidonate and prostaglandins is vectorial in mdck epithelial cells
publishDate 1992
url http://sedici.unlp.edu.ar/handle/10915/67401
https://www.sciencedirect.com/science/article/pii/009069809290008H
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AT leitnerpp stimulatedreleaseofarachidonateandprostaglandinsisvectorialinmdckepithelialcells
AT chandraboseka stimulatedreleaseofarachidonateandprostaglandinsisvectorialinmdckepithelialcells
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