Vectorial insulin secretion by pancreatic β-cells

Morphological studies of pancreatic β-cells have suggested the presence of discrete sensory and secretory domains. In the present study we now provide functional evidence by demonstrating polarity of insulin release by HIT-T15 cells. A significant diffusion barrier across a twin chamber culture syst...

Descripción completa

Detalles Bibliográficos
Autores principales: Cortizo, Ana María, Espinal, Joseph, Hammonds, Peter
Formato: Articulo
Lenguaje:Inglés
Publicado: 1990
Materias:
Ciencias Médicas
Insulina
Páncreas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/67399
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-67399
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Insulina
Páncreas
spellingShingle Ciencias Médicas
Insulina
Páncreas
Cortizo, Ana María
Espinal, Joseph
Hammonds, Peter
Vectorial insulin secretion by pancreatic β-cells
topic_facet Ciencias Médicas
Insulina
Páncreas
description Morphological studies of pancreatic β-cells have suggested the presence of discrete sensory and secretory domains. In the present study we now provide functional evidence by demonstrating polarity of insulin release by HIT-T15 cells. A significant diffusion barrier across a twin chamber culture system was verified in the presence of confluent HIT-T15 cells. When stimulated with sulphonylurea, ionophore or high potassium, insulin was preferentially released into the lower chamber irrespective of whether secretagogues were added to the upper or lower chambers. Vectorial insulin secretion may be a significant determinant of islet hormone paracrine interactions in the maintenance of glucose homeostasis.
format Articulo
Articulo
author Cortizo, Ana María
Espinal, Joseph
Hammonds, Peter
author_facet Cortizo, Ana María
Espinal, Joseph
Hammonds, Peter
author_sort Cortizo, Ana María
title Vectorial insulin secretion by pancreatic β-cells
title_short Vectorial insulin secretion by pancreatic β-cells
title_full Vectorial insulin secretion by pancreatic β-cells
title_fullStr Vectorial insulin secretion by pancreatic β-cells
title_full_unstemmed Vectorial insulin secretion by pancreatic β-cells
title_sort vectorial insulin secretion by pancreatic β-cells
publishDate 1990
url http://sedici.unlp.edu.ar/handle/10915/67399
work_keys_str_mv AT cortizoanamaria vectorialinsulinsecretionbypancreaticbcells
AT espinaljoseph vectorialinsulinsecretionbypancreaticbcells
AT hammondspeter vectorialinsulinsecretionbypancreaticbcells
bdutipo_str Repositorios
_version_ 1764820481293156353

Ejemplares similares