Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>

Lactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, saf...

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Detalles Bibliográficos
Autores principales: Carasi, Paula, Díaz, Mariángeles, Racedo, Silvia María, De Antoni, Graciela Liliana, Urdaci, María C., Serradell, María de los Ángeles
Formato: Articulo
Lenguaje:Español
Publicado: 2014
Materias:
Ciencias Exactas
Lactobacillus
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/67064
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-67064
record_format dspace
spelling I19-R120-10915-670642024-06-12T16:01:50Z http://sedici.unlp.edu.ar/handle/10915/67064 Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i> Carasi, Paula Díaz, Mariángeles Racedo, Silvia María De Antoni, Graciela Liliana Urdaci, María C. Serradell, María de los Ángeles 2014 2018-05-28T16:11:19Z es Ciencias Exactas Lactobacillus Lactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, safety aspects and antimicrobial activity of L. kefiri strains were studied. None of the L. kefiri strains tested caused - or -hemolysis. All the strains were susceptible to tetracycline, clindamycin, streptomycin, ampicillin, erythromycin, kanamycin, and gentamicin; meanwhile, two strains were resistant to chloramphenicol. On the other hand, all L. kefiri strains were able to inhibit both Gram(+) and Gram(−) pathogens. Regarding the in vitro results, L. kefiri CIDCA 8348 was selected to perform in vivo studies. Mice treated daily with an oral dose of 108 CFU during 21 days showed no signs of pain, lethargy, dehydration, or diarrhea, and the histological studies were consistent with those findings. Moreover, no differences in proinflammatory cytokines secretion were observed between treated and control mice. No translocation of microorganisms to blood, spleen, or liver was observed. Regarding these findings, L. kefiri CIDCA 8348 is a microorganism isolated from a dairy product with a great potential as probiotic for human or animal use. Facultad de Ciencias Exactas Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Ciencias Exactas
Lactobacillus
spellingShingle Ciencias Exactas
Lactobacillus
Carasi, Paula
Díaz, Mariángeles
Racedo, Silvia María
De Antoni, Graciela Liliana
Urdaci, María C.
Serradell, María de los Ángeles
Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
topic_facet Ciencias Exactas
Lactobacillus
description Lactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, safety aspects and antimicrobial activity of L. kefiri strains were studied. None of the L. kefiri strains tested caused - or -hemolysis. All the strains were susceptible to tetracycline, clindamycin, streptomycin, ampicillin, erythromycin, kanamycin, and gentamicin; meanwhile, two strains were resistant to chloramphenicol. On the other hand, all L. kefiri strains were able to inhibit both Gram(+) and Gram(−) pathogens. Regarding the in vitro results, L. kefiri CIDCA 8348 was selected to perform in vivo studies. Mice treated daily with an oral dose of 108 CFU during 21 days showed no signs of pain, lethargy, dehydration, or diarrhea, and the histological studies were consistent with those findings. Moreover, no differences in proinflammatory cytokines secretion were observed between treated and control mice. No translocation of microorganisms to blood, spleen, or liver was observed. Regarding these findings, L. kefiri CIDCA 8348 is a microorganism isolated from a dairy product with a great potential as probiotic for human or animal use.
format Articulo
Articulo
author Carasi, Paula
Díaz, Mariángeles
Racedo, Silvia María
De Antoni, Graciela Liliana
Urdaci, María C.
Serradell, María de los Ángeles
author_facet Carasi, Paula
Díaz, Mariángeles
Racedo, Silvia María
De Antoni, Graciela Liliana
Urdaci, María C.
Serradell, María de los Ángeles
author_sort Carasi, Paula
title Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
title_short Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
title_full Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
title_fullStr Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
title_full_unstemmed Safety Characterization and Antimicrobial Properties of Kefir-Isolated <i>Lactobacillus kefiri</i>
title_sort safety characterization and antimicrobial properties of kefir-isolated <i>lactobacillus kefiri</i>
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/67064
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