Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys

Paenibacillus larvae, the causal agent of American foulbrood disease in honeybees, acquires tetracycline-resistance via native plasmids carrying known tetracycline-resistance determinants. From three P. larvae tetracycline-resistant strains isolated from honeys, 5-kb-circular plasmids with almost id...

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Autores principales:	Alippi, Adriana Mónica, León, Ignacio Esteban, López, Ana Claudia
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2014
Materias:	Ciencias Agrarias Abejas fitopatología loque americana Plásmidos Tetraciclina Paenibacillus
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/45190 http://revistes.iec.cat/index.php/IM/article/viewFile/85964/85666
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-45190
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Agrarias Abejas fitopatología loque americana Plásmidos Tetraciclina Paenibacillus
spellingShingle	Ciencias Agrarias Abejas fitopatología loque americana Plásmidos Tetraciclina Paenibacillus Alippi, Adriana Mónica León, Ignacio Esteban López, Ana Claudia Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
topic_facet	Ciencias Agrarias Abejas fitopatología loque americana Plásmidos Tetraciclina Paenibacillus
description	Paenibacillus larvae, the causal agent of American foulbrood disease in honeybees, acquires tetracycline-resistance via native plasmids carrying known tetracycline-resistance determinants. From three P. larvae tetracycline-resistant strains isolated from honeys, 5-kb-circular plasmids with almost identical sequences, designated pPL373 in strain PL373, pPL374 in strain PL374, and pPL395 in strain PL395, were isolated. These plasmids were highly similar (99%) to small tetracycline- encoding plasmids (pMA67, pBHS24, pBSDMV46A, pDMV2, pSU1, pAST4, and pLS55) that replicate by the rolling circle mechanism. Nucleotide sequences comparisons showed that pPL373, pPL374, and pPL395 mainly differed from the previously reported P. larvae plasmid pMA67 in the oriT region and mob genes. These differences suggest alternative mobilization and/or conjugation capacities. Plasmids pPL373, pPL374, and pPL395 were individually transferred by electroporation and stably maintained in tetracycline-susceptible P. larvae NRRL B-14154, in which they autonomously replicated. The presence of nearly identical plasmids in five different genera of gram-positive bacteria, i.e., Bhargavaea, Bacillus, Lactobacillus, Paenibacillus, and Sporosarcina, inhabiting diverse ecological niches provides further evidence of the genetic transfer of tetracycline resistance among environmental bacteria from soils, food, and marine habitats and from pathogenic bacteria such as P. larvae.
format	Articulo Articulo
author	Alippi, Adriana Mónica León, Ignacio Esteban López, Ana Claudia
author_facet	Alippi, Adriana Mónica León, Ignacio Esteban López, Ana Claudia
author_sort	Alippi, Adriana Mónica
title	Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
title_short	Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
title_full	Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
title_fullStr	Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
title_full_unstemmed	Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
title_sort	tetracycline-resistance encoding plasmids from <i>paenibacillus larvae</i>, the causal agent of american foulbrood disease, isolated from commercial honeys
publishDate	2014
url	http://sedici.unlp.edu.ar/handle/10915/45190 http://revistes.iec.cat/index.php/IM/article/viewFile/85964/85666
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bdutipo_str	Repositorios
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Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys

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