Efeito da <i>Mannheimia granulomatis</i> sobre cultivo de fibroblastos
Primary cultures of <i>Mannheimia granulomatis</i> were established in chicken embryos to assess their capacity to stimulate fibroblast proliferation. The capacity of the bacterium-activated macrophages to stimulate cytokine and enzyme proliferation was assessed in a mouse peritoneum mac...
| Autores principales: | , , , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Portugués |
| Publicado: |
2008
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/36562 http://www.scielo.br/pdf/abmvz/v60n2/a38v60n2.pdf |
| Aporte de: |
| id |
I19-R120-10915-36562 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Portugués |
| topic |
Ciencias Veterinarias fibroblasts bacteria macrophages Mannheimia granulomatis |
| spellingShingle |
Ciencias Veterinarias fibroblasts bacteria macrophages Mannheimia granulomatis Ladeira, S. Gomes, F. R. Vidor, T. Portiansky, Enrique Leo Gimeno, Eduardo Juan Efeito da <i>Mannheimia granulomatis</i> sobre cultivo de fibroblastos |
| topic_facet |
Ciencias Veterinarias fibroblasts bacteria macrophages Mannheimia granulomatis |
| description |
Primary cultures of <i>Mannheimia granulomatis</i> were established in chicken embryos to assess their capacity to stimulate fibroblast proliferation. The capacity of the bacterium-activated macrophages to stimulate cytokine and enzyme proliferation was assessed in a mouse peritoneum macrophage culture. To evaluate the bacteria infection on fibroblasts and their growth within 48h in relation to the active macrophages, cultures were washed and trypsinized and the cells counted. Results showed no significant differences when the bacteria-infected fibroblasts were mixed with bacterial extract (P=0.9682). The treatment using just products of macrophages resulted similar to the negative control. Significant differences on cell proliferation were established (P=0,0039) when the products of <i>M. granulomatis</i> -activated macrophages were used, meaning that bacterial components were unable to promote fibroblast increase. Further research is needed to elucidate the effect of <i>M. granulomatis</i> on the macrophages. |
| format |
Articulo Comunicacion |
| author |
Ladeira, S. Gomes, F. R. Vidor, T. Portiansky, Enrique Leo Gimeno, Eduardo Juan |
| author_facet |
Ladeira, S. Gomes, F. R. Vidor, T. Portiansky, Enrique Leo Gimeno, Eduardo Juan |
| author_sort |
Ladeira, S. |
| title |
Efeito da <i>Mannheimia granulomatis</i> sobre cultivo de fibroblastos |
| title_short |
Efeito da <i>Mannheimia granulomatis</i> sobre cultivo de fibroblastos |
| title_full |
Efeito da <i>Mannheimia granulomatis</i> sobre cultivo de fibroblastos |
| title_fullStr |
Efeito da <i>Mannheimia granulomatis</i> sobre cultivo de fibroblastos |
| title_full_unstemmed |
Efeito da <i>Mannheimia granulomatis</i> sobre cultivo de fibroblastos |
| title_sort |
efeito da <i>mannheimia granulomatis</i> sobre cultivo de fibroblastos |
| publishDate |
2008 |
| url |
http://sedici.unlp.edu.ar/handle/10915/36562 http://www.scielo.br/pdf/abmvz/v60n2/a38v60n2.pdf |
| work_keys_str_mv |
AT ladeiras efeitodaimannheimiagranulomatisisobrecultivodefibroblastos AT gomesfr efeitodaimannheimiagranulomatisisobrecultivodefibroblastos AT vidort efeitodaimannheimiagranulomatisisobrecultivodefibroblastos AT portianskyenriqueleo efeitodaimannheimiagranulomatisisobrecultivodefibroblastos AT gimenoeduardojuan efeitodaimannheimiagranulomatisisobrecultivodefibroblastos AT ladeiras effectofmannheimiagranulomatisonfibroblastoscultures AT gomesfr effectofmannheimiagranulomatisonfibroblastoscultures AT vidort effectofmannheimiagranulomatisonfibroblastoscultures AT portianskyenriqueleo effectofmannheimiagranulomatisonfibroblastoscultures AT gimenoeduardojuan effectofmannheimiagranulomatisonfibroblastoscultures |
| bdutipo_str |
Repositorios |
| _version_ |
1764820471352655874 |