Immunohistochemical detection of <i>Tritrichomonas foetus</i> in experimentally infected mice
The need to intensify knowledge of the pathogenesis of bovine genital trichomoniasis (BGT) led to the use of alternative animal models such as the mouse. Nevertheless, it is necessary to elucidate the dynamics of the infection in this animal species, evaluating different stages of the colonization a...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2000
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/35944 http://www.scielo.br/pdf/pvb/v20n1/1403.pdf |
| Aporte de: |
| id |
I19-R120-10915-35944 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Veterinarias bovine genital trichomoniasis immunohistochemistry mouse Tritrichomonas foetus |
| spellingShingle |
Ciencias Veterinarias bovine genital trichomoniasis immunohistochemistry mouse Tritrichomonas foetus Monteavaro, Cristina Esther Soto, Pedro Echevarría, Hilda María Catena, María del Carmen Portiansky, Enrique Leo Gimeno, Eduardo Juan Immunohistochemical detection of <i>Tritrichomonas foetus</i> in experimentally infected mice |
| topic_facet |
Ciencias Veterinarias bovine genital trichomoniasis immunohistochemistry mouse Tritrichomonas foetus |
| description |
The need to intensify knowledge of the pathogenesis of bovine genital trichomoniasis (BGT) led to the use of alternative animal models such as the mouse. Nevertheless, it is necessary to elucidate the dynamics of the infection in this animal species, evaluating different stages of the colonization and evolution of the pathological alterations. The immunohistochemistry (IHC) offers advantages over the routine histopathological staining techniques for the detection of the protozoan in tissues, cellular detritus and inside the macrophages. The goal of the present study was to demonstrate the presence of <i>Tritrichomonas foetus</i> in the reproductive tract of infected mice using an IHC technique. Female BALB/c mice were infected with a suspension of <i>T. foetus</i> by intravaginal route, in the estrum phase, detected by exfoliative vaginal cytology. After 10 weeks, the animals were sacrificed; uterus and vagina were fixed and histologically processed. Some slides were stained with HE. The rest of the slides were processed for IHC. An immunoadsorbed polyclonal serum against <i>T. foetus</i> was used. The avidine-biotine technique (HistoMouse, Zymed™) was employed. The histopathological studies showed a dilation of the uterine glands, presence of macrophages in the lumen of the organ and inner part of the endometrial glands. No <i>T. foetus</i> was identified using this method. The IHQ allowed additionally the identification of the protozoan in the endometrium, endometrial glands, uterine lumen and inside neutrophils and macrophages. The cytological studies stained with IHC showed either isolated <i>T. foetus</i> adhered to epithelial cells or inside macrophages. This technique proves to be a useful tool for the study of the pathogenesis of bovine genital trichomoniasis (BGT) in an experimental model. |
| format |
Articulo Articulo |
| author |
Monteavaro, Cristina Esther Soto, Pedro Echevarría, Hilda María Catena, María del Carmen Portiansky, Enrique Leo Gimeno, Eduardo Juan |
| author_facet |
Monteavaro, Cristina Esther Soto, Pedro Echevarría, Hilda María Catena, María del Carmen Portiansky, Enrique Leo Gimeno, Eduardo Juan |
| author_sort |
Monteavaro, Cristina Esther |
| title |
Immunohistochemical detection of <i>Tritrichomonas foetus</i> in experimentally infected mice |
| title_short |
Immunohistochemical detection of <i>Tritrichomonas foetus</i> in experimentally infected mice |
| title_full |
Immunohistochemical detection of <i>Tritrichomonas foetus</i> in experimentally infected mice |
| title_fullStr |
Immunohistochemical detection of <i>Tritrichomonas foetus</i> in experimentally infected mice |
| title_full_unstemmed |
Immunohistochemical detection of <i>Tritrichomonas foetus</i> in experimentally infected mice |
| title_sort |
immunohistochemical detection of <i>tritrichomonas foetus</i> in experimentally infected mice |
| publishDate |
2000 |
| url |
http://sedici.unlp.edu.ar/handle/10915/35944 http://www.scielo.br/pdf/pvb/v20n1/1403.pdf |
| work_keys_str_mv |
AT monteavarocristinaesther immunohistochemicaldetectionofitritrichomonasfoetusiinexperimentallyinfectedmice AT sotopedro immunohistochemicaldetectionofitritrichomonasfoetusiinexperimentallyinfectedmice AT echevarriahildamaria immunohistochemicaldetectionofitritrichomonasfoetusiinexperimentallyinfectedmice AT catenamariadelcarmen immunohistochemicaldetectionofitritrichomonasfoetusiinexperimentallyinfectedmice AT portianskyenriqueleo immunohistochemicaldetectionofitritrichomonasfoetusiinexperimentallyinfectedmice AT gimenoeduardojuan immunohistochemicaldetectionofitritrichomonasfoetusiinexperimentallyinfectedmice |
| bdutipo_str |
Repositorios |
| _version_ |
1764820472280645636 |