Hormonal induction of spawning in 4 species of frogs by coinjection with a gonadotropin-releasing hormone agonist and a dopamine antagonist

Background: It is well known that many anurans do not reproduce easily in captivity. Some methods are based on administration of mammalian hormones such as human chorionic gonadotropin, which are not effective in many frogs. There is a need for simple, cost-effective alternative techniques to induce...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Trudeau, Vance L., Somoza, Gustavo M., Natale, Guillermo Sebastián, Pauli, Bruce, Wignall, Jacqui, Jackman, Paula, Doe, Ken, Schueler, Fredrick W.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Ciencias Naturales
Bioquímica
Anuros
Hormonas
Reproducción
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/29419
http://www.rbej.com/content/8/1/36
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-29419
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Bioquímica
Anuros
Hormonas
Reproducción
spellingShingle Ciencias Naturales
Bioquímica
Anuros
Hormonas
Reproducción
Trudeau, Vance L.
Somoza, Gustavo M.
Natale, Guillermo Sebastián
Pauli, Bruce
Wignall, Jacqui
Jackman, Paula
Doe, Ken
Schueler, Fredrick W.
Hormonal induction of spawning in 4 species of frogs by coinjection with a gonadotropin-releasing hormone agonist and a dopamine antagonist
topic_facet Ciencias Naturales
Bioquímica
Anuros
Hormonas
Reproducción
description Background: It is well known that many anurans do not reproduce easily in captivity. Some methods are based on administration of mammalian hormones such as human chorionic gonadotropin, which are not effective in many frogs. There is a need for simple, cost-effective alternative techniques to induce spawning.Methods: Our new method is based on the injection of a combination of a gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist. We have named this formulation AMPHIPLEX, which is derived from the combination of the words amphibian and amplexus. This name refers to the specific reproductive behavior of frogs when the male mounts and clasps the female to induce ovulation and to fertilize the eggs as they are laid.Results: We describe the use of the method and demonstrate its applicability for captive breeding in 3 different anuran families. We tested several combinations of GnRH agonists with dopamine antagonists using Lithobates pipiens. The combination of des-Gly10, D-Ala6, Pro-LHRH (0.4 microrams/g body weight) and metoclopramide (10 micrograms/g BWt. MET) was most effective. It was used in-season, after short-term captivity and in frogs artificially hibernated under laboratory conditions. The AMPHIPLEX method was also effective in 3 Argentinian frogs, Ceratophrys ornata, Ceratophrys cranwelli and Odontophrynus americanus.Conclusion: Our approach offers some advantages over other hormonally-based techniques. Both sexes are injected only once and at the same time, reducing handling stress. AMPHIPLEX is a new reproductive management tool for captive breeding in Anura.
format Articulo
Articulo
author Trudeau, Vance L.
Somoza, Gustavo M.
Natale, Guillermo Sebastián
Pauli, Bruce
Wignall, Jacqui
Jackman, Paula
Doe, Ken
Schueler, Fredrick W.
author_facet Trudeau, Vance L.
Somoza, Gustavo M.
Natale, Guillermo Sebastián
Pauli, Bruce
Wignall, Jacqui
Jackman, Paula
Doe, Ken
Schueler, Fredrick W.
author_sort Trudeau, Vance L.
title Hormonal induction of spawning in 4 species of frogs by coinjection with a gonadotropin-releasing hormone agonist and a dopamine antagonist
title_short Hormonal induction of spawning in 4 species of frogs by coinjection with a gonadotropin-releasing hormone agonist and a dopamine antagonist
title_full Hormonal induction of spawning in 4 species of frogs by coinjection with a gonadotropin-releasing hormone agonist and a dopamine antagonist
title_fullStr Hormonal induction of spawning in 4 species of frogs by coinjection with a gonadotropin-releasing hormone agonist and a dopamine antagonist
title_full_unstemmed Hormonal induction of spawning in 4 species of frogs by coinjection with a gonadotropin-releasing hormone agonist and a dopamine antagonist
title_sort hormonal induction of spawning in 4 species of frogs by coinjection with a gonadotropin-releasing hormone agonist and a dopamine antagonist
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/29419
http://www.rbej.com/content/8/1/36
work_keys_str_mv AT trudeauvancel hormonalinductionofspawningin4speciesoffrogsbycoinjectionwithagonadotropinreleasinghormoneagonistandadopamineantagonist
AT somozagustavom hormonalinductionofspawningin4speciesoffrogsbycoinjectionwithagonadotropinreleasinghormoneagonistandadopamineantagonist
AT nataleguillermosebastian hormonalinductionofspawningin4speciesoffrogsbycoinjectionwithagonadotropinreleasinghormoneagonistandadopamineantagonist
AT paulibruce hormonalinductionofspawningin4speciesoffrogsbycoinjectionwithagonadotropinreleasinghormoneagonistandadopamineantagonist
AT wignalljacqui hormonalinductionofspawningin4speciesoffrogsbycoinjectionwithagonadotropinreleasinghormoneagonistandadopamineantagonist
AT jackmanpaula hormonalinductionofspawningin4speciesoffrogsbycoinjectionwithagonadotropinreleasinghormoneagonistandadopamineantagonist
AT doeken hormonalinductionofspawningin4speciesoffrogsbycoinjectionwithagonadotropinreleasinghormoneagonistandadopamineantagonist
AT schuelerfredrickw hormonalinductionofspawningin4speciesoffrogsbycoinjectionwithagonadotropinreleasinghormoneagonistandadopamineantagonist
bdutipo_str Repositorios
_version_ 1764820468139819009

Ejemplares similares