Determination of emodin, chrysophanol, and physcion by HPLC in the chinese medicine rumex japonicus houtt.

A high performance liquid chromatography (HPLC) method for determination of emodin, chrysophanol and physcion in Rumex japonicus Houtt. from different places in Zhejiang Province (China) was established. The samples were separated on a Agilent Zorbax SB-C18 column (2.1 × 150 mm, 5 μm) at at a flow r...

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Detalles Bibliográficos
Autores principales: Lin, Chongliang, Chen, Mengchun, Cai, Jinzhang, Lai, Minmin, Zheng, Yiting, Yang, Xuezhi
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Emodin
Chrysophanol
HPLC
Physcion
Rumex japonicus Houtt
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/25727
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_2_2.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-25727
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Emodin
Chrysophanol
HPLC
Physcion
Rumex japonicus Houtt
spellingShingle Farmacia
Emodin
Chrysophanol
HPLC
Physcion
Rumex japonicus Houtt
Lin, Chongliang
Chen, Mengchun
Cai, Jinzhang
Lai, Minmin
Zheng, Yiting
Yang, Xuezhi
Determination of emodin, chrysophanol, and physcion by HPLC in the chinese medicine rumex japonicus houtt.
topic_facet Farmacia
Emodin
Chrysophanol
HPLC
Physcion
Rumex japonicus Houtt
description A high performance liquid chromatography (HPLC) method for determination of emodin, chrysophanol and physcion in Rumex japonicus Houtt. from different places in Zhejiang Province (China) was established. The samples were separated on a Agilent Zorbax SB-C18 column (2.1 × 150 mm, 5 μm) at at a flow rate of 0.4 mL/min using acetonitrile-water as the mobile phase, with a gradient elution. The column oven temperature was 30 °C and the wavelength of detection used was 285 nm. The three anthraquinones were well separated by this HPLC method. Linearities of emodin, chrysophanol, and physcion were good in the ranges of 0.23-46 (r = 0.998), 0.2-50 (r = 0.9999), and 0.5-50 (r = 0.9997) μg/mL, respectively. The average recoveries were 99.01 % for emodin, 100.73 % for chrysophanol, 101.12 % for physcion, with RSD 1.23, 1.09 and 3.42 %, respectively. The method is simple, accurate, reproducible, and it can be used to quality control of Rumex japonicus Houtt.
format Articulo
Comunicacion
author Lin, Chongliang
Chen, Mengchun
Cai, Jinzhang
Lai, Minmin
Zheng, Yiting
Yang, Xuezhi
author_facet Lin, Chongliang
Chen, Mengchun
Cai, Jinzhang
Lai, Minmin
Zheng, Yiting
Yang, Xuezhi
author_sort Lin, Chongliang
title Determination of emodin, chrysophanol, and physcion by HPLC in the chinese medicine rumex japonicus houtt.
title_short Determination of emodin, chrysophanol, and physcion by HPLC in the chinese medicine rumex japonicus houtt.
title_full Determination of emodin, chrysophanol, and physcion by HPLC in the chinese medicine rumex japonicus houtt.
title_fullStr Determination of emodin, chrysophanol, and physcion by HPLC in the chinese medicine rumex japonicus houtt.
title_full_unstemmed Determination of emodin, chrysophanol, and physcion by HPLC in the chinese medicine rumex japonicus houtt.
title_sort determination of emodin, chrysophanol, and physcion by hplc in the chinese medicine rumex japonicus houtt.
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/25727
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_2_2.pdf
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