Determination of bupropion and hydroxybupropion by LC-MS/MS in rat and its application to assessment of CYP2B6 activity

A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantitation of bupropion (BUP) and its metabolite hydroxybupropion (HBUP) in rat plasma and urine using carbamazepine as an internal standard. Chromatographic separation was achieved on a...

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Autores principales: Pan, Xiaofeng, Xu, Zhisheng, Du, Xiaoxiang, Hu, Hufeng, Fei, Ai-li, Wang, Zhe, Xu, Ren-ai
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Bupropion
Sistema Enzimático del Citocromo P-450
Plasma
Cromatografía
Espectrometría de Masas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/25697
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_1_10.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-25697
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Bupropion
Sistema Enzimático del Citocromo P-450
Plasma
Cromatografía
Espectrometría de Masas
spellingShingle Farmacia
Bupropion
Sistema Enzimático del Citocromo P-450
Plasma
Cromatografía
Espectrometría de Masas
Pan, Xiaofeng
Xu, Zhisheng
Du, Xiaoxiang
Hu, Hufeng
Fei, Ai-li
Wang, Zhe
Xu, Ren-ai
Determination of bupropion and hydroxybupropion by LC-MS/MS in rat and its application to assessment of CYP2B6 activity
topic_facet Farmacia
Bupropion
Sistema Enzimático del Citocromo P-450
Plasma
Cromatografía
Espectrometría de Masas
description A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantitation of bupropion (BUP) and its metabolite hydroxybupropion (HBUP) in rat plasma and urine using carbamazepine as an internal standard. Chromatographic separation was achieved on a SB-C18 column at 30 °C, using the gradient elution of 0.1 % formic acid in water and acetonitrile. Calibration plots were linear over range of 10-2000 ng/mL for BUP and 5-1000 ng/mL for HBUP in rat plasma. The intra- and inter-run relative standard deviations of the assay were less than 10 % for both BUP and HBUP. The effects of tolbutamide on cytochrome P450-mediated metabolism of BUP were studied by the validated method above. The results revealed that tolbutamide had signigicantly decreased the rate of BUP hydroxylation.
format Articulo
Articulo
author Pan, Xiaofeng
Xu, Zhisheng
Du, Xiaoxiang
Hu, Hufeng
Fei, Ai-li
Wang, Zhe
Xu, Ren-ai
author_facet Pan, Xiaofeng
Xu, Zhisheng
Du, Xiaoxiang
Hu, Hufeng
Fei, Ai-li
Wang, Zhe
Xu, Ren-ai
author_sort Pan, Xiaofeng
title Determination of bupropion and hydroxybupropion by LC-MS/MS in rat and its application to assessment of CYP2B6 activity
title_short Determination of bupropion and hydroxybupropion by LC-MS/MS in rat and its application to assessment of CYP2B6 activity
title_full Determination of bupropion and hydroxybupropion by LC-MS/MS in rat and its application to assessment of CYP2B6 activity
title_fullStr Determination of bupropion and hydroxybupropion by LC-MS/MS in rat and its application to assessment of CYP2B6 activity
title_full_unstemmed Determination of bupropion and hydroxybupropion by LC-MS/MS in rat and its application to assessment of CYP2B6 activity
title_sort determination of bupropion and hydroxybupropion by lc-ms/ms in rat and its application to assessment of cyp2b6 activity
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/25697
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_1_10.pdf
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