Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes

This study was performed to screen α-glucosidase inhibitors from the actinomycete metabolites library by high throughput screening. Twelve strains of actinomycete were considered to be α-glucosidase inhibitors producing strains; then effective inhibitory strain PW409 was fermented and separated by b...

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Detalles Bibliográficos
Autores principales:	Wang, Liqiang, Hou, Yuan-Yuan, Peng, Jiamin, Qi, Xizhen, Zhang, Qi, Bai, Fang, Bai, Gang
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2012
Materias:	Farmacia Actinomycetes Bioactivity-based HPLC α-glucosidase inhibitor Identification Quantification Screening alfa-Glucosidasas
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/20220 http://www.latamjpharm.org/resumenes/31/5/LAJOP_31_5_1_8.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-20220
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia Actinomycetes Bioactivity-based HPLC α-glucosidase inhibitor Identification Quantification Screening alfa-Glucosidasas
spellingShingle	Farmacia Actinomycetes Bioactivity-based HPLC α-glucosidase inhibitor Identification Quantification Screening alfa-Glucosidasas Wang, Liqiang Hou, Yuan-Yuan Peng, Jiamin Qi, Xizhen Zhang, Qi Bai, Fang Bai, Gang Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
topic_facet	Farmacia Actinomycetes Bioactivity-based HPLC α-glucosidase inhibitor Identification Quantification Screening alfa-Glucosidasas
description	This study was performed to screen α-glucosidase inhibitors from the actinomycete metabolites library by high throughput screening. Twelve strains of actinomycete were considered to be α-glucosidase inhibitors producing strains; then effective inhibitory strain PW409 was fermented and separated by bioactivity based HPLC, two fractions showing remarkable inhibitory activities; the two compounds were identified as 1-deoxynojirimycin (DNJ) and miglitol by mass spectrometry, comparing with authentic standards, and relevant literature. The quantification analysis of DNJ and miglitol by HPLC-MS/MS showed that the average concentrations of DNJ and miglitol in broth of strain PW409 were 11.2 and 95.8 mg/L, respectively. This is the first report about Streptomyces sp. products α-glucosidase inhibitor miglitol. The strain PW409 has potential application in biosynthesis and biotransformation of antidiabetes drug miglitol. The method can be utilized for new α-glucosidase inhibitors discovering and development from other inhibitory activity strains.
format	Articulo Articulo
author	Wang, Liqiang Hou, Yuan-Yuan Peng, Jiamin Qi, Xizhen Zhang, Qi Bai, Fang Bai, Gang
author_facet	Wang, Liqiang Hou, Yuan-Yuan Peng, Jiamin Qi, Xizhen Zhang, Qi Bai, Fang Bai, Gang
author_sort	Wang, Liqiang
title	Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
title_short	Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
title_full	Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
title_fullStr	Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
title_full_unstemmed	Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
title_sort	bioactivity-based hplc tandem q/tof for alpha-glucosidase inhibitors : screening, identification, and quantification from actinomycetes
publishDate	2012
url	http://sedici.unlp.edu.ar/handle/10915/20220 http://www.latamjpharm.org/resumenes/31/5/LAJOP_31_5_1_8.pdf
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bdutipo_str	Repositorios
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Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes

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