Simultaneous determination of midazolam and 1’-hydroxymidazolam in rat plasma by protein precipitation and LC-MS: application to pharmacokinetic study

A sensitive and selective liquid chromatography–mass spectrometry (LC–MS) method for determination of midazolam and its metabolite 1’-hydroxymidazolam in rat plasma was developed and validated. After addition of carbamazepine as internal standard (IS), protein precipitation by acetonitrile was used...

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Autores principales:	Chen, Xiaole, Xu, Tao, Wang, Zhe, Pan, Peipei, Luo, Shunbing, Lin, Dan, Hu, Guoxin, Pan, Jianchun
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2012
Materias:	Farmacia 1’-hydroxymidazolam LC–MS Midazolam Plasma Espectrometría de Masas Farmacocinética
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/20174 http://www.latamjpharm.org/resumenes/31/4/LAJOP_31_4_1_6.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-20174
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia 1’-hydroxymidazolam LC–MS Midazolam Plasma Espectrometría de Masas Farmacocinética
spellingShingle	Farmacia 1’-hydroxymidazolam LC–MS Midazolam Plasma Espectrometría de Masas Farmacocinética Chen, Xiaole Xu, Tao Wang, Zhe Pan, Peipei Luo, Shunbing Lin, Dan Hu, Guoxin Pan, Jianchun Simultaneous determination of midazolam and 1’-hydroxymidazolam in rat plasma by protein precipitation and LC-MS: application to pharmacokinetic study
topic_facet	Farmacia 1’-hydroxymidazolam LC–MS Midazolam Plasma Espectrometría de Masas Farmacocinética
description	A sensitive and selective liquid chromatography–mass spectrometry (LC–MS) method for determination of midazolam and its metabolite 1’-hydroxymidazolam in rat plasma was developed and validated. After addition of carbamazepine as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. The chromatographic separation was performed on a Zorbax SB-C18 column (150 × 2.1 mm, 5 μm), using acetonitrile-0.1 % formic acid as the mobile phase with gradient elution, delivered at a ﬂow-rate of 0.4 mL/min. Electrospray ionization (ESI) source was applied and operated in positive ion mode, and selected ion monitoring (SIM) mode used to quantify midazolam and its metabolite 1’- hydroxymidazolam. Calibration curves were linear in the concentration ranges of 5-2000 ng/mL for midazolam and 10-2000 ng/mL for 1’-hydroxymidazolam, with a lower limit of quantification (LLOQ) of 5 ng/mL for midazolam and 10 ng/mL for 1’-hydroxymidazolam, respectively. Intra- and inter-day precision were less than 13 % and the accuracy ranged from -10.7 to 9.5 %. This developed method was successfully used for determination of midazolam and its metabolite 1’-hydroxymidazolam in rat plasma for pharmacokinetic study.
format	Articulo Articulo
author	Chen, Xiaole Xu, Tao Wang, Zhe Pan, Peipei Luo, Shunbing Lin, Dan Hu, Guoxin Pan, Jianchun
author_facet	Chen, Xiaole Xu, Tao Wang, Zhe Pan, Peipei Luo, Shunbing Lin, Dan Hu, Guoxin Pan, Jianchun
author_sort	Chen, Xiaole
title	Simultaneous determination of midazolam and 1’-hydroxymidazolam in rat plasma by protein precipitation and LC-MS: application to pharmacokinetic study
title_short	Simultaneous determination of midazolam and 1’-hydroxymidazolam in rat plasma by protein precipitation and LC-MS: application to pharmacokinetic study
title_full	Simultaneous determination of midazolam and 1’-hydroxymidazolam in rat plasma by protein precipitation and LC-MS: application to pharmacokinetic study
title_fullStr	Simultaneous determination of midazolam and 1’-hydroxymidazolam in rat plasma by protein precipitation and LC-MS: application to pharmacokinetic study
title_full_unstemmed	Simultaneous determination of midazolam and 1’-hydroxymidazolam in rat plasma by protein precipitation and LC-MS: application to pharmacokinetic study
title_sort	simultaneous determination of midazolam and 1’-hydroxymidazolam in rat plasma by protein precipitation and lc-ms: application to pharmacokinetic study
publishDate	2012
url	http://sedici.unlp.edu.ar/handle/10915/20174 http://www.latamjpharm.org/resumenes/31/4/LAJOP_31_4_1_6.pdf
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Simultaneous determination of midazolam and 1’-hydroxymidazolam in rat plasma by protein precipitation and LC-MS: application to pharmacokinetic study

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