Determination of erlotinib in rabbit plasma by liquid chromatography mass spectrometry

A sensitive and selective liquid chromatography mass spectrometry (LC–MS) method for determination of erlotinib in rabbit plasma was developed. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was...

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Autores principales: Ding, Xitao, Zhang, Chongxiao, Xu, Zhisheng, Zhang, Qingwei, Wu, Haiya, Wang, Zhiyi, Ma, Jianshe
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Erlotinib
LC-MS
Pharmacokinetics
Plasma
Espectrometría de Masas
Farmacocinética
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/20168
http://www.latamjpharm.org/resumenes/31/4/LAJOP_31_4_1_1.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-20168
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Erlotinib
LC-MS
Pharmacokinetics
Plasma
Espectrometría de Masas
Farmacocinética
spellingShingle Farmacia
Erlotinib
LC-MS
Pharmacokinetics
Plasma
Espectrometría de Masas
Farmacocinética
Ding, Xitao
Zhang, Chongxiao
Xu, Zhisheng
Zhang, Qingwei
Wu, Haiya
Wang, Zhiyi
Ma, Jianshe
Determination of erlotinib in rabbit plasma by liquid chromatography mass spectrometry
topic_facet Farmacia
Erlotinib
LC-MS
Pharmacokinetics
Plasma
Espectrometría de Masas
Farmacocinética
description A sensitive and selective liquid chromatography mass spectrometry (LC–MS) method for determination of erlotinib in rabbit plasma was developed. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1 × 150 mm, 5 μm) column with acetonitrile-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; multiple reaction monitoring (MRM) mode was used to quantification using target fragment ions m/z 394→336 for erlotinib and m/z 326→291 for the IS. Calibration plots were linear over the range of 5-2000 ng/mL for erlotinib in plasma. Lower limit of quantification (LLOQ) for erlotinib was 5 ng/mL. Mean recovery of erlotinib from plasma was in the range 84.5-95.7 %. CV of intra-day and interday precision were both less than 12 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of erlotinib in rabbit plasma.
format Articulo
Articulo
author Ding, Xitao
Zhang, Chongxiao
Xu, Zhisheng
Zhang, Qingwei
Wu, Haiya
Wang, Zhiyi
Ma, Jianshe
author_facet Ding, Xitao
Zhang, Chongxiao
Xu, Zhisheng
Zhang, Qingwei
Wu, Haiya
Wang, Zhiyi
Ma, Jianshe
author_sort Ding, Xitao
title Determination of erlotinib in rabbit plasma by liquid chromatography mass spectrometry
title_short Determination of erlotinib in rabbit plasma by liquid chromatography mass spectrometry
title_full Determination of erlotinib in rabbit plasma by liquid chromatography mass spectrometry
title_fullStr Determination of erlotinib in rabbit plasma by liquid chromatography mass spectrometry
title_full_unstemmed Determination of erlotinib in rabbit plasma by liquid chromatography mass spectrometry
title_sort determination of erlotinib in rabbit plasma by liquid chromatography mass spectrometry
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/20168
http://www.latamjpharm.org/resumenes/31/4/LAJOP_31_4_1_1.pdf
work_keys_str_mv AT dingxitao determinationoferlotinibinrabbitplasmabyliquidchromatographymassspectrometry
AT zhangchongxiao determinationoferlotinibinrabbitplasmabyliquidchromatographymassspectrometry
AT xuzhisheng determinationoferlotinibinrabbitplasmabyliquidchromatographymassspectrometry
AT zhangqingwei determinationoferlotinibinrabbitplasmabyliquidchromatographymassspectrometry
AT wuhaiya determinationoferlotinibinrabbitplasmabyliquidchromatographymassspectrometry
AT wangzhiyi determinationoferlotinibinrabbitplasmabyliquidchromatographymassspectrometry
AT majianshe determinationoferlotinibinrabbitplasmabyliquidchromatographymassspectrometry
bdutipo_str Repositorios
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