Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application
A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of cilostazol in rat plasma using one-step protein precipitation was developed. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as s...
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Autores principales: | , , , , , , , |
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Formato: | Articulo |
Lenguaje: | Inglés |
Publicado: |
2012
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/18097 http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_10.pdf |
Aporte de: |
id |
I19-R120-10915-18097 |
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record_format |
dspace |
institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Farmacia cilostazol; gradient elution; LC-ESI-MS; rat plasma cilostazo |
spellingShingle |
Farmacia cilostazol; gradient elution; LC-ESI-MS; rat plasma cilostazo Wang, Jinjin Wang, Zhibin Wang, Zhiyi Chen, Chan Liu, Naihua Ma, Jianshe Wang, Xianqin Hu, Lufeng Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application |
topic_facet |
Farmacia cilostazol; gradient elution; LC-ESI-MS; rat plasma cilostazo |
description |
A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of cilostazol in rat plasma using one-step protein precipitation was developed.
After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 × 50 mm, 5.0 μm) column with acetonitrile-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 370.0 for cilostazol and m/z 325.9 for the IS. Calibration plots were linear over the range of 10-2000 ng/mL for cilostazol in rat plasma. Lower limit of quantification (LLOQ) for cilostazol was 10 ng/mL. Mean recovery of cilostazol from plasma was in the range 90.14- 95.10 %. RSD of intra-day and inter-day precision were both less than 15 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of cilostazol in rat plasma. |
format |
Articulo Articulo |
author |
Wang, Jinjin Wang, Zhibin Wang, Zhiyi Chen, Chan Liu, Naihua Ma, Jianshe Wang, Xianqin Hu, Lufeng |
author_facet |
Wang, Jinjin Wang, Zhibin Wang, Zhiyi Chen, Chan Liu, Naihua Ma, Jianshe Wang, Xianqin Hu, Lufeng |
author_sort |
Wang, Jinjin |
title |
Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application |
title_short |
Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application |
title_full |
Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application |
title_fullStr |
Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application |
title_full_unstemmed |
Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application |
title_sort |
gradient elution lc-esi-ms determination of cilostazol in rat plasma and its application |
publishDate |
2012 |
url |
http://sedici.unlp.edu.ar/handle/10915/18097 http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_10.pdf |
work_keys_str_mv |
AT wangjinjin gradientelutionlcesimsdeterminationofcilostazolinratplasmaanditsapplication AT wangzhibin gradientelutionlcesimsdeterminationofcilostazolinratplasmaanditsapplication AT wangzhiyi gradientelutionlcesimsdeterminationofcilostazolinratplasmaanditsapplication AT chenchan gradientelutionlcesimsdeterminationofcilostazolinratplasmaanditsapplication AT liunaihua gradientelutionlcesimsdeterminationofcilostazolinratplasmaanditsapplication AT majianshe gradientelutionlcesimsdeterminationofcilostazolinratplasmaanditsapplication AT wangxianqin gradientelutionlcesimsdeterminationofcilostazolinratplasmaanditsapplication AT hulufeng gradientelutionlcesimsdeterminationofcilostazolinratplasmaanditsapplication |
bdutipo_str |
Repositorios |
_version_ |
1764820463788228608 |