Oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells
The applicability of ultrasmall uncapped and aminosilanized oxidized silicon nanoparticles (SiNPs and NH2-SiNPs) as radiosensitizer was studied by internalizing these nanoparticles into human breast cancer (MCF-7) and mouse fibroblast cells (3T3) that were exposed to X-rays at a single dose of 3 Gy....
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| Formato: | Articulo |
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2013
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/170145 |
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I19-R120-10915-170145 |
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I19-R120-10915-1701452024-09-14T04:08:45Z http://sedici.unlp.edu.ar/handle/10915/170145 Oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells Klein, Stefanie Dell'Arciprete, María Laura Wegmann, Marc Distel, Luitpold V.R. Neuhuber, Winfried González, Mónica Cristina Kryschi, Carola 2013 2024-09-13T15:19:38Z en Bioquímica Química Silicon nanoparticles Radiosensitizer Oxidative stress Reactive oxygen species The applicability of ultrasmall uncapped and aminosilanized oxidized silicon nanoparticles (SiNPs and NH2-SiNPs) as radiosensitizer was studied by internalizing these nanoparticles into human breast cancer (MCF-7) and mouse fibroblast cells (3T3) that were exposed to X-rays at a single dose of 3 Gy. While SiNPs did not increase the production of reactive oxygen species (ROS) in X-ray treated cells, the NH2-S1NPs significantly enhanced the ROS formation. This is due to the amino functionality as providing positive surface charges in aqueous environment. The NH2-S1NPs were observed to penetrate into the mitochondrial membrane, wherein these nanoparticles provoked oxidative stress. The NH2-SiNPs induced mitochondrial ROS production was confirmed by the determination of an increased malondialdehyde level as representing a gauge for the extent of membrane lipid peroxidation. X-ray exposure of NH2-SiNPs incubated MCF-7 and 3T3 cells increased the ROS concentration for 180%, and 120%, respectively. Complementary cytotoxicity studies demonstrate that these silicon nanoparticles are more cytotoxic for MCF- 7 than for 3T3 cells. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 217-222 |
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Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Bioquímica Química Silicon nanoparticles Radiosensitizer Oxidative stress Reactive oxygen species |
| spellingShingle |
Bioquímica Química Silicon nanoparticles Radiosensitizer Oxidative stress Reactive oxygen species Klein, Stefanie Dell'Arciprete, María Laura Wegmann, Marc Distel, Luitpold V.R. Neuhuber, Winfried González, Mónica Cristina Kryschi, Carola Oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells |
| topic_facet |
Bioquímica Química Silicon nanoparticles Radiosensitizer Oxidative stress Reactive oxygen species |
| description |
The applicability of ultrasmall uncapped and aminosilanized oxidized silicon nanoparticles (SiNPs and NH2-SiNPs) as radiosensitizer was studied by internalizing these nanoparticles into human breast cancer (MCF-7) and mouse fibroblast cells (3T3) that were exposed to X-rays at a single dose of 3 Gy. While SiNPs did not increase the production of reactive oxygen species (ROS) in X-ray treated cells, the NH2-S1NPs significantly enhanced the ROS formation. This is due to the amino functionality as providing positive surface charges in aqueous environment. The NH2-S1NPs were observed to penetrate into the mitochondrial membrane, wherein these nanoparticles provoked oxidative stress. The NH2-SiNPs induced mitochondrial ROS production was confirmed by the determination of an increased malondialdehyde level as representing a gauge for the extent of membrane lipid peroxidation. X-ray exposure of NH2-SiNPs incubated MCF-7 and 3T3 cells increased the ROS concentration for 180%, and 120%, respectively. Complementary cytotoxicity studies demonstrate that these silicon nanoparticles are more cytotoxic for MCF- 7 than for 3T3 cells. |
| format |
Articulo Articulo |
| author |
Klein, Stefanie Dell'Arciprete, María Laura Wegmann, Marc Distel, Luitpold V.R. Neuhuber, Winfried González, Mónica Cristina Kryschi, Carola |
| author_facet |
Klein, Stefanie Dell'Arciprete, María Laura Wegmann, Marc Distel, Luitpold V.R. Neuhuber, Winfried González, Mónica Cristina Kryschi, Carola |
| author_sort |
Klein, Stefanie |
| title |
Oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells |
| title_short |
Oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells |
| title_full |
Oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells |
| title_fullStr |
Oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells |
| title_full_unstemmed |
Oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells |
| title_sort |
oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells |
| publishDate |
2013 |
| url |
http://sedici.unlp.edu.ar/handle/10915/170145 |
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