Prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in Pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in Lotus tenuis

A bioprospecting study in alkaline-sodic soils of the Argentinean flooding pampa was performed in order to identify and characterize rhizospheric bacteria associated to Lotus tenuis plants, capable of solubilizing phosphate under a broad range of alkaline-sodic conditions. Our analysis, supported by...

Descripción completa

Detalles Bibliográficos
Autores principales: Cumpa-Velásquez, Liz Marjory, Moriconi, Jorge Ignacio, Dip, Diana Patricia, Castagno, Luis Nazareno, Puig, María Lucrecia, Maiale, Santiago Javier, Santa María, Guillermo Esteban, Sannazzaro, Analía Inés, Estrella, María Julia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Ciencias Agrarias
Phosphobacteria
Restrictive soils
Symbiosis
Taxonomic diversity
Nutrition
Legume
Plant growth promotion
Agriculture
Biofertilizers
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/170108
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-170108
record_format dspace
spelling I19-R120-10915-1701082024-09-12T20:05:16Z http://sedici.unlp.edu.ar/handle/10915/170108 Prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in Pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in Lotus tenuis Cumpa-Velásquez, Liz Marjory Moriconi, Jorge Ignacio Dip, Diana Patricia Castagno, Luis Nazareno Puig, María Lucrecia Maiale, Santiago Javier Santa María, Guillermo Esteban Sannazzaro, Analía Inés Estrella, María Julia 2021 2024-09-12T17:59:58Z en Ciencias Agrarias Phosphobacteria Restrictive soils Symbiosis Taxonomic diversity Nutrition Legume Plant growth promotion Agriculture Biofertilizers A bioprospecting study in alkaline-sodic soils of the Argentinean flooding pampa was performed in order to identify and characterize rhizospheric bacteria associated to Lotus tenuis plants, capable of solubilizing phosphate under a broad range of alkaline-sodic conditions. Our analysis, supported by repetitive BOX element based PCR and 16S rRNA sequences, identified 74 strains. All of them belong to the Phylum Proteobacteria, specifically to the order Enterobacteriales, and Pseudomonadales, suggesting that in this environment, broad pH-range P-solubilizing bacteria (BRPSB) associated to L. tenuis, are grouped within a narrow taxonomic range. A subsequent objective was to focus in a subgroup of BRPSB strains belonging to the Pantoea eucalypti species (MA66, P63, P76, P163, P173 and a formerly identified isolate, M91) that also produced siderophores, indol-acetic acid and showed in vitro compatibility with the native rhizobial strain Mesorhizobium sanjuanii BSA136. Growth promoting effects of these P. eucalypti strains on L. tenuis plants in alkaline-sodic soils in symbiosis with the above mentioned rhizobial strain were analyzed. Despite all the P. eucalypti BRPSB strains exhibited the above-mentioned features, they exerted differential effects on plant growth and dry matter allocation to the nodules. Plants inoculated with P. eucalypti M91 displayed a superior capability to accumulate nitrogen, phosphorus and zinc. On the contrary, nodules dry matter allocation, and mineral nutrient accumulation in L. tenuis plants were negatively affected by P. eucalypti P76 compared with M91. Results hereby presented highlight the complexity of plant-microbe interactions and reveal that growth-promoting effects of P-solubilizing P. eucalypti strains cannot be predicted only on the basis of their in vitro PGPR features, complementary in planta assays being necessary for efficient strain selection. This study provides valuable information for biofertilization of L. tenuis plants in the flooding pampa. Facultad de Ciencias Agrarias y Forestales Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Phosphobacteria
Restrictive soils
Symbiosis
Taxonomic diversity
Nutrition
Legume
Plant growth promotion
Agriculture
Biofertilizers
spellingShingle Ciencias Agrarias
Phosphobacteria
Restrictive soils
Symbiosis
Taxonomic diversity
Nutrition
Legume
Plant growth promotion
Agriculture
Biofertilizers
Cumpa-Velásquez, Liz Marjory
Moriconi, Jorge Ignacio
Dip, Diana Patricia
Castagno, Luis Nazareno
Puig, María Lucrecia
Maiale, Santiago Javier
Santa María, Guillermo Esteban
Sannazzaro, Analía Inés
Estrella, María Julia
Prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in Pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in Lotus tenuis
topic_facet Ciencias Agrarias
Phosphobacteria
Restrictive soils
Symbiosis
Taxonomic diversity
Nutrition
Legume
Plant growth promotion
Agriculture
Biofertilizers
description A bioprospecting study in alkaline-sodic soils of the Argentinean flooding pampa was performed in order to identify and characterize rhizospheric bacteria associated to Lotus tenuis plants, capable of solubilizing phosphate under a broad range of alkaline-sodic conditions. Our analysis, supported by repetitive BOX element based PCR and 16S rRNA sequences, identified 74 strains. All of them belong to the Phylum Proteobacteria, specifically to the order Enterobacteriales, and Pseudomonadales, suggesting that in this environment, broad pH-range P-solubilizing bacteria (BRPSB) associated to L. tenuis, are grouped within a narrow taxonomic range. A subsequent objective was to focus in a subgroup of BRPSB strains belonging to the Pantoea eucalypti species (MA66, P63, P76, P163, P173 and a formerly identified isolate, M91) that also produced siderophores, indol-acetic acid and showed in vitro compatibility with the native rhizobial strain Mesorhizobium sanjuanii BSA136. Growth promoting effects of these P. eucalypti strains on L. tenuis plants in alkaline-sodic soils in symbiosis with the above mentioned rhizobial strain were analyzed. Despite all the P. eucalypti BRPSB strains exhibited the above-mentioned features, they exerted differential effects on plant growth and dry matter allocation to the nodules. Plants inoculated with P. eucalypti M91 displayed a superior capability to accumulate nitrogen, phosphorus and zinc. On the contrary, nodules dry matter allocation, and mineral nutrient accumulation in L. tenuis plants were negatively affected by P. eucalypti P76 compared with M91. Results hereby presented highlight the complexity of plant-microbe interactions and reveal that growth-promoting effects of P-solubilizing P. eucalypti strains cannot be predicted only on the basis of their in vitro PGPR features, complementary in planta assays being necessary for efficient strain selection. This study provides valuable information for biofertilization of L. tenuis plants in the flooding pampa.
format Articulo
Articulo
author Cumpa-Velásquez, Liz Marjory
Moriconi, Jorge Ignacio
Dip, Diana Patricia
Castagno, Luis Nazareno
Puig, María Lucrecia
Maiale, Santiago Javier
Santa María, Guillermo Esteban
Sannazzaro, Analía Inés
Estrella, María Julia
author_facet Cumpa-Velásquez, Liz Marjory
Moriconi, Jorge Ignacio
Dip, Diana Patricia
Castagno, Luis Nazareno
Puig, María Lucrecia
Maiale, Santiago Javier
Santa María, Guillermo Esteban
Sannazzaro, Analía Inés
Estrella, María Julia
author_sort Cumpa-Velásquez, Liz Marjory
title Prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in Pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in Lotus tenuis
title_short Prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in Pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in Lotus tenuis
title_full Prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in Pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in Lotus tenuis
title_fullStr Prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in Pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in Lotus tenuis
title_full_unstemmed Prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in Pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in Lotus tenuis
title_sort prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in lotus tenuis
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/170108
work_keys_str_mv AT cumpavelasquezlizmarjory prospectingphosphatesolubilizingbacteriainalkalinesodicenvironmentsrevealsintraspecificvariabilityinpantoeaeucalyptiaffectingnutrientacquisitionandrhizobialnodulationinlotustenuis
AT moriconijorgeignacio prospectingphosphatesolubilizingbacteriainalkalinesodicenvironmentsrevealsintraspecificvariabilityinpantoeaeucalyptiaffectingnutrientacquisitionandrhizobialnodulationinlotustenuis
AT dipdianapatricia prospectingphosphatesolubilizingbacteriainalkalinesodicenvironmentsrevealsintraspecificvariabilityinpantoeaeucalyptiaffectingnutrientacquisitionandrhizobialnodulationinlotustenuis
AT castagnoluisnazareno prospectingphosphatesolubilizingbacteriainalkalinesodicenvironmentsrevealsintraspecificvariabilityinpantoeaeucalyptiaffectingnutrientacquisitionandrhizobialnodulationinlotustenuis
AT puigmarialucrecia prospectingphosphatesolubilizingbacteriainalkalinesodicenvironmentsrevealsintraspecificvariabilityinpantoeaeucalyptiaffectingnutrientacquisitionandrhizobialnodulationinlotustenuis
AT maialesantiagojavier prospectingphosphatesolubilizingbacteriainalkalinesodicenvironmentsrevealsintraspecificvariabilityinpantoeaeucalyptiaffectingnutrientacquisitionandrhizobialnodulationinlotustenuis
AT santamariaguillermoesteban prospectingphosphatesolubilizingbacteriainalkalinesodicenvironmentsrevealsintraspecificvariabilityinpantoeaeucalyptiaffectingnutrientacquisitionandrhizobialnodulationinlotustenuis
AT sannazzaroanaliaines prospectingphosphatesolubilizingbacteriainalkalinesodicenvironmentsrevealsintraspecificvariabilityinpantoeaeucalyptiaffectingnutrientacquisitionandrhizobialnodulationinlotustenuis
AT estrellamariajulia prospectingphosphatesolubilizingbacteriainalkalinesodicenvironmentsrevealsintraspecificvariabilityinpantoeaeucalyptiaffectingnutrientacquisitionandrhizobialnodulationinlotustenuis
_version_ 1825276023893131264

Ejemplares similares