The distinct cell physiology of Bradyrhizobium at the population and cellular level

The α-Proteobacteria belonging to Bradyrhizobium genus are microorganisms of extreme slow growth. Despite their extended use as inoculants in soybean production, their physiology remains poorly characterized. In this work, we produced quantitative data on four different isolates: B. diazoefficens US...

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Detalles Bibliográficos
Autores principales: Medici, Ian F., Bartrolí, Leila, Guaimas, Francisco F., Fulgenzi, Fabiana R., Molina, Charo Luciana, Sánchez, Ignacio Enrique, Comerci, Diego J., Mongiardini, Elías Javier, Soler-Bistué, Alfonso
Formato: Articulo
Lenguaje:Inglés
Publicado: 2024
Materias:
Biología
Bradyrhizobium
Bacterial physiology
Growth rate
Cell polarity
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/167729
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-167729
record_format dspace
spelling I19-R120-10915-1677292024-07-03T20:08:42Z http://sedici.unlp.edu.ar/handle/10915/167729 The distinct cell physiology of Bradyrhizobium at the population and cellular level Medici, Ian F. Bartrolí, Leila Guaimas, Francisco F. Fulgenzi, Fabiana R. Molina, Charo Luciana Sánchez, Ignacio Enrique Comerci, Diego J. Mongiardini, Elías Javier Soler-Bistué, Alfonso 2024 2024-07-03T15:21:28Z en Biología Bradyrhizobium Bacterial physiology Growth rate Cell polarity The α-Proteobacteria belonging to Bradyrhizobium genus are microorganisms of extreme slow growth. Despite their extended use as inoculants in soybean production, their physiology remains poorly characterized. In this work, we produced quantitative data on four different isolates: B. diazoefficens USDA110, B. diazoefficiens USDA122, B. japonicum E109 and B. japonicum USDA6 which are representative of specific genomic profiles. Notably, we found conserved physiological traits conserved in all the studied isolates: (i) the lag and initial exponential growth phases display cell aggregation; (ii) the increase in specific nutrient concentration such as yeast extract and gluconate hinders growth; (iii) cell size does not correlate with culture age; and (iv) cell cycle presents polar growth. Meanwhile, fitness, cell size and in vitro growth widely vary across isolates correlating to ribosomal RNA operon number. In summary, this study provides novel empirical data that enriches the comprehension of the Bradyrhizobium (slow) growth dynamics and cell cycle. Instituto de Biotecnología y Biología Molecular Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bradyrhizobium
Bacterial physiology
Growth rate
Cell polarity
spellingShingle Biología
Bradyrhizobium
Bacterial physiology
Growth rate
Cell polarity
Medici, Ian F.
Bartrolí, Leila
Guaimas, Francisco F.
Fulgenzi, Fabiana R.
Molina, Charo Luciana
Sánchez, Ignacio Enrique
Comerci, Diego J.
Mongiardini, Elías Javier
Soler-Bistué, Alfonso
The distinct cell physiology of Bradyrhizobium at the population and cellular level
topic_facet Biología
Bradyrhizobium
Bacterial physiology
Growth rate
Cell polarity
description The α-Proteobacteria belonging to Bradyrhizobium genus are microorganisms of extreme slow growth. Despite their extended use as inoculants in soybean production, their physiology remains poorly characterized. In this work, we produced quantitative data on four different isolates: B. diazoefficens USDA110, B. diazoefficiens USDA122, B. japonicum E109 and B. japonicum USDA6 which are representative of specific genomic profiles. Notably, we found conserved physiological traits conserved in all the studied isolates: (i) the lag and initial exponential growth phases display cell aggregation; (ii) the increase in specific nutrient concentration such as yeast extract and gluconate hinders growth; (iii) cell size does not correlate with culture age; and (iv) cell cycle presents polar growth. Meanwhile, fitness, cell size and in vitro growth widely vary across isolates correlating to ribosomal RNA operon number. In summary, this study provides novel empirical data that enriches the comprehension of the Bradyrhizobium (slow) growth dynamics and cell cycle.
format Articulo
Articulo
author Medici, Ian F.
Bartrolí, Leila
Guaimas, Francisco F.
Fulgenzi, Fabiana R.
Molina, Charo Luciana
Sánchez, Ignacio Enrique
Comerci, Diego J.
Mongiardini, Elías Javier
Soler-Bistué, Alfonso
author_facet Medici, Ian F.
Bartrolí, Leila
Guaimas, Francisco F.
Fulgenzi, Fabiana R.
Molina, Charo Luciana
Sánchez, Ignacio Enrique
Comerci, Diego J.
Mongiardini, Elías Javier
Soler-Bistué, Alfonso
author_sort Medici, Ian F.
title The distinct cell physiology of Bradyrhizobium at the population and cellular level
title_short The distinct cell physiology of Bradyrhizobium at the population and cellular level
title_full The distinct cell physiology of Bradyrhizobium at the population and cellular level
title_fullStr The distinct cell physiology of Bradyrhizobium at the population and cellular level
title_full_unstemmed The distinct cell physiology of Bradyrhizobium at the population and cellular level
title_sort distinct cell physiology of bradyrhizobium at the population and cellular level
publishDate 2024
url http://sedici.unlp.edu.ar/handle/10915/167729
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