Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies

In the present work the creation of a new biomaterial aimed at bone tissue regeneration is shown, covering the synthesis of the polymers, the material design and their exhaustive physicochemical and biological characterization. The copolymers of dioctyl fumarate (DOF) and N-isopropylacrylamide (NIPA...

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Autores principales: Bravi Costantino, Maria Leticia, Cortizo, María Susana, Cortizo, Ana María, Oberti, Tamara Gisela
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Química
Biología
fumaric copolymers
thermal properties
fibrous scaffold
biocompatibility
osteoblastic differentiation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/163964
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-163964
record_format dspace
spelling I19-R120-10915-1639642024-03-19T04:07:25Z http://sedici.unlp.edu.ar/handle/10915/163964 Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies Bravi Costantino, Maria Leticia Cortizo, María Susana Cortizo, Ana María Oberti, Tamara Gisela 2020-01 2024-03-18T17:01:46Z en Química Biología fumaric copolymers thermal properties fibrous scaffold biocompatibility osteoblastic differentiation In the present work the creation of a new biomaterial aimed at bone tissue regeneration is shown, covering the synthesis of the polymers, the material design and their exhaustive physicochemical and biological characterization. The copolymers of dioctyl fumarate (DOF) and N-isopropylacrylamide (NIPAM) were synthetized and characterized by size exclusion chromatography, 1H NMR and its thermal properties were studied by DSC and TGA analyses. Based on their properties, two copolymers with different DOF content were selected as raw material to design scaffolds for bone tissue regeneration. The scaffolds were prepared by casting and electrospinning technique, giving rise to biomaterials with particular chemical and topographic surface which were characterized by water contact angle and scanning electron microscopy (SEM) analysis. The cytotoxicity and biodegradation by murine macrophage RAW 264.7 cells was assay, while biocompatibility and osteogenic induction were made using bone marrow progenitor cells (BMPC). None of the biomaterials obtained showed cytotoxicity. The biological properties suggest that the cells adhere and proliferate better on biomaterials with higher DOF content and fibrous morphology. All scaffolds support osteoblastic cell differentiation, but to a greater extent on the biomaterial that has higher DOF content prepared by casting. Altogether, our results suggesting that these biomaterials could be useful in the bone tissue engineering. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Laboratorio de Investigación en Osteopatías y Metabolismo Mineral Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Biología
fumaric copolymers
thermal properties
fibrous scaffold
biocompatibility
osteoblastic differentiation
spellingShingle Química
Biología
fumaric copolymers
thermal properties
fibrous scaffold
biocompatibility
osteoblastic differentiation
Bravi Costantino, Maria Leticia
Cortizo, María Susana
Cortizo, Ana María
Oberti, Tamara Gisela
Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
topic_facet Química
Biología
fumaric copolymers
thermal properties
fibrous scaffold
biocompatibility
osteoblastic differentiation
description In the present work the creation of a new biomaterial aimed at bone tissue regeneration is shown, covering the synthesis of the polymers, the material design and their exhaustive physicochemical and biological characterization. The copolymers of dioctyl fumarate (DOF) and N-isopropylacrylamide (NIPAM) were synthetized and characterized by size exclusion chromatography, 1H NMR and its thermal properties were studied by DSC and TGA analyses. Based on their properties, two copolymers with different DOF content were selected as raw material to design scaffolds for bone tissue regeneration. The scaffolds were prepared by casting and electrospinning technique, giving rise to biomaterials with particular chemical and topographic surface which were characterized by water contact angle and scanning electron microscopy (SEM) analysis. The cytotoxicity and biodegradation by murine macrophage RAW 264.7 cells was assay, while biocompatibility and osteogenic induction were made using bone marrow progenitor cells (BMPC). None of the biomaterials obtained showed cytotoxicity. The biological properties suggest that the cells adhere and proliferate better on biomaterials with higher DOF content and fibrous morphology. All scaffolds support osteoblastic cell differentiation, but to a greater extent on the biomaterial that has higher DOF content prepared by casting. Altogether, our results suggesting that these biomaterials could be useful in the bone tissue engineering.
format Articulo
Articulo
author Bravi Costantino, Maria Leticia
Cortizo, María Susana
Cortizo, Ana María
Oberti, Tamara Gisela
author_facet Bravi Costantino, Maria Leticia
Cortizo, María Susana
Cortizo, Ana María
Oberti, Tamara Gisela
author_sort Bravi Costantino, Maria Leticia
title Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
title_short Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
title_full Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
title_fullStr Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
title_full_unstemmed Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
title_sort osteogenic scaffolds based on fumaric/n-isopropylacrylamide copolymers: designed, properties and biocompatibility studies
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/163964
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AT cortizoanamaria osteogenicscaffoldsbasedonfumaricnisopropylacrylamidecopolymersdesignedpropertiesandbiocompatibilitystudies
AT obertitamaragisela osteogenicscaffoldsbasedonfumaricnisopropylacrylamidecopolymersdesignedpropertiesandbiocompatibilitystudies
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