Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation
There is an urgent need to address the shortage of potential multivisceral grafts in order to reduce the average time in waiting list. Since donation after circulatory death (DCD) has been successfully employed for other solid organs, a thorough evaluation of the use of intestinal grafts from DCD is...
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2023
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/162848 |
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I19-R120-10915-162848 |
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I19-R120-10915-1628482024-02-19T20:08:22Z http://sedici.unlp.edu.ar/handle/10915/162848 Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation Stringa, Pablo Luis Vecchio Dezillio, Leandro Emmanuel Talayero, Paloma Serradilla, Javier Errea, Agustina Juliana Papa Gobbi, Rodrigo Camps Ortega, Onys Pucci Molineris, Melisa Eliana Lausada, Natalia Raquel Andres Moreno, Ane Miren Rumbo, Martín Hernández Oliveros, Francisco 2023-01-12 2024-02-19T15:57:15Z en Ciencias Médicas donation after cardiac death experimental transplantation intestinal transplantation organ procurement solid organ transplant There is an urgent need to address the shortage of potential multivisceral grafts in order to reduce the average time in waiting list. Since donation after circulatory death (DCD) has been successfully employed for other solid organs, a thorough evaluation of the use of intestinal grafts from DCD is warranted. Here, we have generated a model of Maastricht III DCD in rodents, focusing on the viability of intestinal and multivisceral grafts at five (DCD5) and twenty (DCD20) minutes of cardiac arrest compared to living and brain death donors. DCD groups exhibited time-dependent damage. DCD20 generated substantial intestinal mucosal injury and decreased number of Goblet cells whereas grafts from DCD5 closely resemble those of brain death and living donors groups in terms intestinal morphology, expression of tight junction proteins and number of Paneth and Globet cells. Upon transplantation, intestines from DCD5 showed increased ischemia/reperfusion damage compared to living donor grafts, however mucosal integrity was recovered 48 h after transplantation. No differences in terms of graft rejection, gene expression and absorptive function between DCD5 and living donor were observed at 7 post-transplant days. Collectively, our results highlight DCD as a possible strategy to increase multivisceral donation and transplantation procedures. Instituto de Estudios Inmunológicos y Fisiopatológicos Consejo Nacional de Investigaciones Científicas y Técnicas Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Médicas donation after cardiac death experimental transplantation intestinal transplantation organ procurement solid organ transplant |
| spellingShingle |
Ciencias Médicas donation after cardiac death experimental transplantation intestinal transplantation organ procurement solid organ transplant Stringa, Pablo Luis Vecchio Dezillio, Leandro Emmanuel Talayero, Paloma Serradilla, Javier Errea, Agustina Juliana Papa Gobbi, Rodrigo Camps Ortega, Onys Pucci Molineris, Melisa Eliana Lausada, Natalia Raquel Andres Moreno, Ane Miren Rumbo, Martín Hernández Oliveros, Francisco Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation |
| topic_facet |
Ciencias Médicas donation after cardiac death experimental transplantation intestinal transplantation organ procurement solid organ transplant |
| description |
There is an urgent need to address the shortage of potential multivisceral grafts in order to reduce the average time in waiting list. Since donation after circulatory death (DCD) has been successfully employed for other solid organs, a thorough evaluation of the use of intestinal grafts from DCD is warranted. Here, we have generated a model of Maastricht III DCD in rodents, focusing on the viability of intestinal and multivisceral grafts at five (DCD5) and twenty (DCD20) minutes of cardiac arrest compared to living and brain death donors. DCD groups exhibited time-dependent damage. DCD20 generated substantial intestinal mucosal injury and decreased number of Goblet cells whereas grafts from DCD5 closely resemble those of brain death and living donors groups in terms intestinal morphology, expression of tight junction proteins and number of Paneth and Globet cells. Upon transplantation, intestines from DCD5 showed increased ischemia/reperfusion damage compared to living donor grafts, however mucosal integrity was recovered 48 h after transplantation. No differences in terms of graft rejection, gene expression and absorptive function between DCD5 and living donor were observed at 7 post-transplant days. Collectively, our results highlight DCD as a possible strategy to increase multivisceral donation and transplantation procedures. |
| format |
Articulo Articulo |
| author |
Stringa, Pablo Luis Vecchio Dezillio, Leandro Emmanuel Talayero, Paloma Serradilla, Javier Errea, Agustina Juliana Papa Gobbi, Rodrigo Camps Ortega, Onys Pucci Molineris, Melisa Eliana Lausada, Natalia Raquel Andres Moreno, Ane Miren Rumbo, Martín Hernández Oliveros, Francisco |
| author_facet |
Stringa, Pablo Luis Vecchio Dezillio, Leandro Emmanuel Talayero, Paloma Serradilla, Javier Errea, Agustina Juliana Papa Gobbi, Rodrigo Camps Ortega, Onys Pucci Molineris, Melisa Eliana Lausada, Natalia Raquel Andres Moreno, Ane Miren Rumbo, Martín Hernández Oliveros, Francisco |
| author_sort |
Stringa, Pablo Luis |
| title |
Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation |
| title_short |
Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation |
| title_full |
Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation |
| title_fullStr |
Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation |
| title_full_unstemmed |
Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation |
| title_sort |
experimental assessment of intestinal damage in controlled donation after circulatory death for visceral transplantation |
| publishDate |
2023 |
| url |
http://sedici.unlp.edu.ar/handle/10915/162848 |
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