Cytotoxic and genotoxic assessments of 2,4-dichlorophenoxyacetic acid (2,4-D) in in vitro mammalian cells

A combined approach employing alkaline single cell gel electrophoresis (SCGE) and cytokinesis-blocked micronucleus (MNs) cytome bioassays was adopted to assess the deleterious properties of the auxinic 2,4-dichlorophenoxyacetic acid (2,4-D) and its microparticulated low volatility product Dedalo Eli...

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Autores principales: Laborde, Milagros Rosa Raquel, Larramendy, Marcelo Luis, Soloneski, Sonia María Elsa
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Naturales
2,4-D
Apoptosis
CBMN-cyt assay
CHO-K1 cells
DNA single-strand breaks
Microparticulated commercial formulation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/161709
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-161709
record_format dspace
spelling I19-R120-10915-1617092023-12-19T20:07:46Z http://sedici.unlp.edu.ar/handle/10915/161709 Cytotoxic and genotoxic assessments of 2,4-dichlorophenoxyacetic acid (2,4-D) in in vitro mammalian cells Laborde, Milagros Rosa Raquel Larramendy, Marcelo Luis Soloneski, Sonia María Elsa 2020 2023-12-19T18:43:27Z en Ciencias Naturales 2,4-D Apoptosis CBMN-cyt assay CHO-K1 cells DNA single-strand breaks Microparticulated commercial formulation A combined approach employing alkaline single cell gel electrophoresis (SCGE) and cytokinesis-blocked micronucleus (MNs) cytome bioassays was adopted to assess the deleterious properties of the auxinic 2,4-dichlorophenoxyacetic acid (2,4-D) and its microparticulated low volatility product Dedalo Elite (30% a.i.) on Chinese hamster ovary (CHO-K1) cells. Cytotoxicity was estimated by neutral red uptake (NRU), succinic dehydrogenase activity (MTT) and apoptosis assessment. Both compounds were assayed at 0.1–10 μg/ml concentration range. Whereas exposed CHO-K1 cells revealed a statistically significant enhancement of MNs when 10 μg 2,4-D/ml was assayed, MNs were only achieved in cells treated with 2 μg Dedalo Elite/ml. A diminution in the nuclear division index was only achieved after exposure to Dedalo Elite within the 1–10 μg/ml concentration range. Whereas increased genetic damage index was achieved when 6 and 10 μg 2,4-D/ml were assayed, GDI induction was observed in treatments employing 4 μg Dedalo Elite/ml. Both compounds induced cytotoxicity by inhibition of both lysosomal and MTT activities by enhancing the frequencies of early and late apoptotic cells. Our results not only indicate the genotoxic and cytotoxic potential of 2,4-D and its microparticulated marketplace formulation, but also highlight the risk of these agrochemicals present towards the biota and human health. Facultad de Ciencias Naturales y Museo Consejo Nacional de Investigaciones Científicas y Técnicas Articulo Articulo http://creativecommons.org/licenses/by-nc-nd/4.0/ Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
2,4-D
Apoptosis
CBMN-cyt assay
CHO-K1 cells
DNA single-strand breaks
Microparticulated commercial formulation
spellingShingle Ciencias Naturales
2,4-D
Apoptosis
CBMN-cyt assay
CHO-K1 cells
DNA single-strand breaks
Microparticulated commercial formulation
Laborde, Milagros Rosa Raquel
Larramendy, Marcelo Luis
Soloneski, Sonia María Elsa
Cytotoxic and genotoxic assessments of 2,4-dichlorophenoxyacetic acid (2,4-D) in in vitro mammalian cells
topic_facet Ciencias Naturales
2,4-D
Apoptosis
CBMN-cyt assay
CHO-K1 cells
DNA single-strand breaks
Microparticulated commercial formulation
description A combined approach employing alkaline single cell gel electrophoresis (SCGE) and cytokinesis-blocked micronucleus (MNs) cytome bioassays was adopted to assess the deleterious properties of the auxinic 2,4-dichlorophenoxyacetic acid (2,4-D) and its microparticulated low volatility product Dedalo Elite (30% a.i.) on Chinese hamster ovary (CHO-K1) cells. Cytotoxicity was estimated by neutral red uptake (NRU), succinic dehydrogenase activity (MTT) and apoptosis assessment. Both compounds were assayed at 0.1–10 μg/ml concentration range. Whereas exposed CHO-K1 cells revealed a statistically significant enhancement of MNs when 10 μg 2,4-D/ml was assayed, MNs were only achieved in cells treated with 2 μg Dedalo Elite/ml. A diminution in the nuclear division index was only achieved after exposure to Dedalo Elite within the 1–10 μg/ml concentration range. Whereas increased genetic damage index was achieved when 6 and 10 μg 2,4-D/ml were assayed, GDI induction was observed in treatments employing 4 μg Dedalo Elite/ml. Both compounds induced cytotoxicity by inhibition of both lysosomal and MTT activities by enhancing the frequencies of early and late apoptotic cells. Our results not only indicate the genotoxic and cytotoxic potential of 2,4-D and its microparticulated marketplace formulation, but also highlight the risk of these agrochemicals present towards the biota and human health.
format Articulo
Articulo
author Laborde, Milagros Rosa Raquel
Larramendy, Marcelo Luis
Soloneski, Sonia María Elsa
author_facet Laborde, Milagros Rosa Raquel
Larramendy, Marcelo Luis
Soloneski, Sonia María Elsa
author_sort Laborde, Milagros Rosa Raquel
title Cytotoxic and genotoxic assessments of 2,4-dichlorophenoxyacetic acid (2,4-D) in in vitro mammalian cells
title_short Cytotoxic and genotoxic assessments of 2,4-dichlorophenoxyacetic acid (2,4-D) in in vitro mammalian cells
title_full Cytotoxic and genotoxic assessments of 2,4-dichlorophenoxyacetic acid (2,4-D) in in vitro mammalian cells
title_fullStr Cytotoxic and genotoxic assessments of 2,4-dichlorophenoxyacetic acid (2,4-D) in in vitro mammalian cells
title_full_unstemmed Cytotoxic and genotoxic assessments of 2,4-dichlorophenoxyacetic acid (2,4-D) in in vitro mammalian cells
title_sort cytotoxic and genotoxic assessments of 2,4-dichlorophenoxyacetic acid (2,4-d) in in vitro mammalian cells
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/161709
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AT larramendymarceloluis cytotoxicandgenotoxicassessmentsof24dichlorophenoxyaceticacid24dininvitromammaliancells
AT soloneskisoniamariaelsa cytotoxicandgenotoxicassessmentsof24dichlorophenoxyaceticacid24dininvitromammaliancells
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