Highest priority critically important antimicrobial-resistant Escherichia coli and Salmonella spp. isolated from pork and chicken meat from Argentina

Between June and September 2023, a total of 80 meat samples (48 and 32 from pork and chicken, respectively) were collected from 16 retail markets in La Plata, Argentina. Antimicrobial susceptibility was evaluated by agar diffusion, and PCR was used to identify resistance genes. All markets were posi...

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Autores principales: Nievas, Hernán Darío, Aurnague, Camila, Iza, Raúl Emilio, Helman, María Elisa, Martínez Zugazúa, Matías, Nievas, Victorio Fabio, Carriquiriborde, Martín, Galli, Lucía, Moredo, Fabiana Alicia
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2023
Materias:
Ciencias Veterinarias
highest priority critically important antimicrobial
Escherichia coli
Salmonella
pork
chicken meat
ESBL
AmpC
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/160915
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-160915
record_format dspace
spelling I19-R120-10915-1609152023-11-30T20:08:18Z http://sedici.unlp.edu.ar/handle/10915/160915 Highest priority critically important antimicrobial-resistant Escherichia coli and Salmonella spp. isolated from pork and chicken meat from Argentina Nievas, Hernán Darío Aurnague, Camila Iza, Raúl Emilio Helman, María Elisa Martínez Zugazúa, Matías Nievas, Victorio Fabio Carriquiriborde, Martín Galli, Lucía Moredo, Fabiana Alicia 2023-11-30 2023-11-30T13:52:47Z en Ciencias Veterinarias highest priority critically important antimicrobial Escherichia coli Salmonella pork chicken meat ESBL AmpC Between June and September 2023, a total of 80 meat samples (48 and 32 from pork and chicken, respectively) were collected from 16 retail markets in La Plata, Argentina. Antimicrobial susceptibility was evaluated by agar diffusion, and PCR was used to identify resistance genes. All markets were positive for at least one resistant E. coli. Eighty-four resistant E. coli and two Salmonella spp. were isolated. Resistance to ciprofloxacin was observed in 65 E. coli, 25 from pork, and 40 from chicken meat. Resistance to cefotaxime was observed in 49 E. coli isolates, 14 from pork, and 35 from chicken meat. Seventy-five E. coli isolates were multidrug resistant; twenty-four isolates showed resistance to four antibiotic classes; and 20 strains were resistant to five antibiotic classes. Resistance was most common to ampicillin (91.7%), tetracycline (78.6%), ciprofloxacin (77.4%), cefotaxime (58.3%), chloramphenicol (48.8%), amoxicillin/clavulanic acid (47.6%), sulfamethoxazole- trimethoprim (45.2%), fosfomycin (32.1%), gentamicin (22.6%), ceftazidime (8.3%), and cefoxitin (4.8%). Fourteen E. coli isolated from chicken meat showed resistance to three of the HPCIA cefotaxime/cefepime, ciprofloxacin, and fosfomycin. Resistance to 3rd and 4th generation cephalosporin was associated with bla CTX-M genes; 12 E. coli were isolated from pork and 33 from chicken meat. bla CTX-M alleles were grouped by specific PCR as follows: 1 bla CTX-M-1/15 ; 17 bla CTX-M-2 ; 5 bla CTX-M-9/14 ; and 5 bla CTX-M-8/25 . The results prove that the presence of resistant E. coli in pork and chicken meat can be a source of multiple resistance genes. Facultad de Ciencias Veterinarias Articulo Preprint http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
highest priority critically important antimicrobial
Escherichia coli
Salmonella
pork
chicken meat
ESBL
AmpC
spellingShingle Ciencias Veterinarias
highest priority critically important antimicrobial
Escherichia coli
Salmonella
pork
chicken meat
ESBL
AmpC
Nievas, Hernán Darío
Aurnague, Camila
Iza, Raúl Emilio
Helman, María Elisa
Martínez Zugazúa, Matías
Nievas, Victorio Fabio
Carriquiriborde, Martín
Galli, Lucía
Moredo, Fabiana Alicia
Highest priority critically important antimicrobial-resistant Escherichia coli and Salmonella spp. isolated from pork and chicken meat from Argentina
topic_facet Ciencias Veterinarias
highest priority critically important antimicrobial
Escherichia coli
Salmonella
pork
chicken meat
ESBL
AmpC
description Between June and September 2023, a total of 80 meat samples (48 and 32 from pork and chicken, respectively) were collected from 16 retail markets in La Plata, Argentina. Antimicrobial susceptibility was evaluated by agar diffusion, and PCR was used to identify resistance genes. All markets were positive for at least one resistant E. coli. Eighty-four resistant E. coli and two Salmonella spp. were isolated. Resistance to ciprofloxacin was observed in 65 E. coli, 25 from pork, and 40 from chicken meat. Resistance to cefotaxime was observed in 49 E. coli isolates, 14 from pork, and 35 from chicken meat. Seventy-five E. coli isolates were multidrug resistant; twenty-four isolates showed resistance to four antibiotic classes; and 20 strains were resistant to five antibiotic classes. Resistance was most common to ampicillin (91.7%), tetracycline (78.6%), ciprofloxacin (77.4%), cefotaxime (58.3%), chloramphenicol (48.8%), amoxicillin/clavulanic acid (47.6%), sulfamethoxazole- trimethoprim (45.2%), fosfomycin (32.1%), gentamicin (22.6%), ceftazidime (8.3%), and cefoxitin (4.8%). Fourteen E. coli isolated from chicken meat showed resistance to three of the HPCIA cefotaxime/cefepime, ciprofloxacin, and fosfomycin. Resistance to 3rd and 4th generation cephalosporin was associated with bla CTX-M genes; 12 E. coli were isolated from pork and 33 from chicken meat. bla CTX-M alleles were grouped by specific PCR as follows: 1 bla CTX-M-1/15 ; 17 bla CTX-M-2 ; 5 bla CTX-M-9/14 ; and 5 bla CTX-M-8/25 . The results prove that the presence of resistant E. coli in pork and chicken meat can be a source of multiple resistance genes.
format Articulo
Preprint
author Nievas, Hernán Darío
Aurnague, Camila
Iza, Raúl Emilio
Helman, María Elisa
Martínez Zugazúa, Matías
Nievas, Victorio Fabio
Carriquiriborde, Martín
Galli, Lucía
Moredo, Fabiana Alicia
author_facet Nievas, Hernán Darío
Aurnague, Camila
Iza, Raúl Emilio
Helman, María Elisa
Martínez Zugazúa, Matías
Nievas, Victorio Fabio
Carriquiriborde, Martín
Galli, Lucía
Moredo, Fabiana Alicia
author_sort Nievas, Hernán Darío
title Highest priority critically important antimicrobial-resistant Escherichia coli and Salmonella spp. isolated from pork and chicken meat from Argentina
title_short Highest priority critically important antimicrobial-resistant Escherichia coli and Salmonella spp. isolated from pork and chicken meat from Argentina
title_full Highest priority critically important antimicrobial-resistant Escherichia coli and Salmonella spp. isolated from pork and chicken meat from Argentina
title_fullStr Highest priority critically important antimicrobial-resistant Escherichia coli and Salmonella spp. isolated from pork and chicken meat from Argentina
title_full_unstemmed Highest priority critically important antimicrobial-resistant Escherichia coli and Salmonella spp. isolated from pork and chicken meat from Argentina
title_sort highest priority critically important antimicrobial-resistant escherichia coli and salmonella spp. isolated from pork and chicken meat from argentina
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/160915
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