Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response

Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in diferent animals, including mice, making B. bronchiseptica the gold-standard model to investigate host– pathogen interaction at the molecular level. B. bronchiseptica utilizes many diferent mechanisms to preci...

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Detalles Bibliográficos
Autores principales: Belhart, Keila, Sisti, Federico, Gestal, Mónica C., Fernández, Julieta
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Biología
Inmunología
Microbiología
Biología molecular
patogénesis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/160042
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-160042
record_format dspace
spelling I19-R120-10915-1600422023-11-10T20:07:34Z http://sedici.unlp.edu.ar/handle/10915/160042 Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response Belhart, Keila Sisti, Federico Gestal, Mónica C. Fernández, Julieta 2023-05-02 2023-11-10T16:44:54Z en Biología Inmunología Microbiología Biología molecular patogénesis Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in diferent animals, including mice, making B. bronchiseptica the gold-standard model to investigate host– pathogen interaction at the molecular level. B. bronchiseptica utilizes many diferent mechanisms to precisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesized by diguanylate cyclases and degraded by phosphodiesterases that regulates the expression of multiple virulence factors including bioflm formation. As in other bacteria, we have previously shown that c-di-GMP regulates motility and bioflm formation in B. bronchiseptica. This work describes the diguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase that promotes bioflm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increased macrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 by macrophages. Our study reveals that BdcB regulates the expression of components of T3SS, an important virulence factor of B. bronchiseptica. The Bb∆bdcB mutant presented increased expression of T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealed that albeit the absence of bdcB did not afect the ability of B. bronchiseptica to infect and colonize the respiratory tract of mice, mice infected with Bb∆bdcB presented a signifcantly higher proinfammatory response than those infected with wild type B. bronchiseptica. Instituto de Biotecnología y Biología Molecular Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Inmunología
Microbiología
Biología molecular
patogénesis
spellingShingle Biología
Inmunología
Microbiología
Biología molecular
patogénesis
Belhart, Keila
Sisti, Federico
Gestal, Mónica C.
Fernández, Julieta
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
topic_facet Biología
Inmunología
Microbiología
Biología molecular
patogénesis
description Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in diferent animals, including mice, making B. bronchiseptica the gold-standard model to investigate host– pathogen interaction at the molecular level. B. bronchiseptica utilizes many diferent mechanisms to precisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesized by diguanylate cyclases and degraded by phosphodiesterases that regulates the expression of multiple virulence factors including bioflm formation. As in other bacteria, we have previously shown that c-di-GMP regulates motility and bioflm formation in B. bronchiseptica. This work describes the diguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase that promotes bioflm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increased macrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 by macrophages. Our study reveals that BdcB regulates the expression of components of T3SS, an important virulence factor of B. bronchiseptica. The Bb∆bdcB mutant presented increased expression of T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealed that albeit the absence of bdcB did not afect the ability of B. bronchiseptica to infect and colonize the respiratory tract of mice, mice infected with Bb∆bdcB presented a signifcantly higher proinfammatory response than those infected with wild type B. bronchiseptica.
format Articulo
Articulo
author Belhart, Keila
Sisti, Federico
Gestal, Mónica C.
Fernández, Julieta
author_facet Belhart, Keila
Sisti, Federico
Gestal, Mónica C.
Fernández, Julieta
author_sort Belhart, Keila
title Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_short Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_full Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_fullStr Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_full_unstemmed Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_sort bordetella bronchiseptica diguanylate cyclase bdcb inhibits the type three secretion system and impacts the immune response
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/160042
work_keys_str_mv AT belhartkeila bordetellabronchisepticadiguanylatecyclasebdcbinhibitsthetypethreesecretionsystemandimpactstheimmuneresponse
AT sistifederico bordetellabronchisepticadiguanylatecyclasebdcbinhibitsthetypethreesecretionsystemandimpactstheimmuneresponse
AT gestalmonicac bordetellabronchisepticadiguanylatecyclasebdcbinhibitsthetypethreesecretionsystemandimpactstheimmuneresponse
AT fernandezjulieta bordetellabronchisepticadiguanylatecyclasebdcbinhibitsthetypethreesecretionsystemandimpactstheimmuneresponse
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