PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms

Baculoviruses are insect-specific pathogens widely used in biotechnology. In particular, the Autographa californica nucleopolyhedrovirus (AcMNPV) has been exploited as a platform for bio-inputs production. This is why the improvement of the technologies used for the production of recombinant baculov...

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Autores principales: Amorós Morales, Leslie Cinthya, Marchesini, Abril, Gómez Bergna, Santiago Manuel, García Fallit, Matías, Tongiani, Silvana E., Vásquez, Larisa, Ferrelli, María Leticia, Videla-Richardson, Guillermo A., Candolfi, Marianela, Romanowski, Víctor, Pidre, Matías Luis
Formato: Articulo
Lenguaje:Español
Publicado: 2023
Materias:
Biología
baculovirus expression vectors
golden gate assembly
bio-inputs
gene therapy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/160011
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-160011
record_format dspace
spelling I19-R120-10915-1600112023-11-10T20:07:36Z http://sedici.unlp.edu.ar/handle/10915/160011 PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms Amorós Morales, Leslie Cinthya Marchesini, Abril Gómez Bergna, Santiago Manuel García Fallit, Matías Tongiani, Silvana E. Vásquez, Larisa Ferrelli, María Leticia Videla-Richardson, Guillermo A. Candolfi, Marianela Romanowski, Víctor Pidre, Matías Luis 2023 2023-11-10T13:08:10Z es Biología baculovirus expression vectors golden gate assembly bio-inputs gene therapy Baculoviruses are insect-specific pathogens widely used in biotechnology. In particular, the Autographa californica nucleopolyhedrovirus (AcMNPV) has been exploited as a platform for bio-inputs production. This is why the improvement of the technologies used for the production of recombinant baculoviruses takes on particular relevance. To achieve this goal, we developed a highly versatile baculoviral transfer vector generation system called PluriBAC. The PluriBAC system consists of three insert entry levels using Golden Gate assembly technology. The wide availability of vectors and sticky ends allows enough versatility to combine more than four different promoters, genes of interest, and terminator sequences. Here, we report not only the rational design of the PluriBAC system but also its use for the generation of baculoviral reporter vectors applied to different fields of biotechnology. We demonstrated that recombinant AcMNPV baculoviruses generated with the PluriBAC system were capable of infecting Spodoptera frugiperda larvae. On the other hand, we found that the recombinant budded virions (BV) generated using our system were capable of transducing different types of tumor and normal cells both in vitro and in vivo. Our findings suggest that the PluriBAC system could constitute a versatile tool for the generation of insecticide and gene therapy vectors. Instituto de Biotecnología y Biología Molecular Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Biología
baculovirus expression vectors
golden gate assembly
bio-inputs
gene therapy
spellingShingle Biología
baculovirus expression vectors
golden gate assembly
bio-inputs
gene therapy
Amorós Morales, Leslie Cinthya
Marchesini, Abril
Gómez Bergna, Santiago Manuel
García Fallit, Matías
Tongiani, Silvana E.
Vásquez, Larisa
Ferrelli, María Leticia
Videla-Richardson, Guillermo A.
Candolfi, Marianela
Romanowski, Víctor
Pidre, Matías Luis
PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms
topic_facet Biología
baculovirus expression vectors
golden gate assembly
bio-inputs
gene therapy
description Baculoviruses are insect-specific pathogens widely used in biotechnology. In particular, the Autographa californica nucleopolyhedrovirus (AcMNPV) has been exploited as a platform for bio-inputs production. This is why the improvement of the technologies used for the production of recombinant baculoviruses takes on particular relevance. To achieve this goal, we developed a highly versatile baculoviral transfer vector generation system called PluriBAC. The PluriBAC system consists of three insert entry levels using Golden Gate assembly technology. The wide availability of vectors and sticky ends allows enough versatility to combine more than four different promoters, genes of interest, and terminator sequences. Here, we report not only the rational design of the PluriBAC system but also its use for the generation of baculoviral reporter vectors applied to different fields of biotechnology. We demonstrated that recombinant AcMNPV baculoviruses generated with the PluriBAC system were capable of infecting Spodoptera frugiperda larvae. On the other hand, we found that the recombinant budded virions (BV) generated using our system were capable of transducing different types of tumor and normal cells both in vitro and in vivo. Our findings suggest that the PluriBAC system could constitute a versatile tool for the generation of insecticide and gene therapy vectors.
format Articulo
Articulo
author Amorós Morales, Leslie Cinthya
Marchesini, Abril
Gómez Bergna, Santiago Manuel
García Fallit, Matías
Tongiani, Silvana E.
Vásquez, Larisa
Ferrelli, María Leticia
Videla-Richardson, Guillermo A.
Candolfi, Marianela
Romanowski, Víctor
Pidre, Matías Luis
author_facet Amorós Morales, Leslie Cinthya
Marchesini, Abril
Gómez Bergna, Santiago Manuel
García Fallit, Matías
Tongiani, Silvana E.
Vásquez, Larisa
Ferrelli, María Leticia
Videla-Richardson, Guillermo A.
Candolfi, Marianela
Romanowski, Víctor
Pidre, Matías Luis
author_sort Amorós Morales, Leslie Cinthya
title PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms
title_short PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms
title_full PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms
title_fullStr PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms
title_full_unstemmed PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms
title_sort pluribac: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/160011
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