Identification, phylogenetic analysis, and genome mining of the tetracycline-resistant Bacillus thuringiensis strain m401 reveal its potential for biotechnological and biocontrol applications

Bacillus thuringiensis is an entomopathogen belonging to the Bacillus cereus clade. We isolated a tetracycline-resistant strain called m401, recovered it from honey, and identified it as Bacillus thuringiensis sv. kumamotoensis based on the average nucleotide identity calculations (ANIb) comparison...

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Autores principales: Alippi, Adriana Mónica, Lamelza, Florencia, Torres Tejerizo, Gonzalo Arturo, Abrahamovich, Eliana, López, Ana Claudia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Biología
Bacillus thuringiensis sv. kumamotoensis
Antimicrobial peptides
Paenibacillus larvae
Tetracycline resistance
tet(45)
Péptidos antimicrobianos
Resistencia a tetraciclina
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/159941
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-159941
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bacillus thuringiensis sv. kumamotoensis
Antimicrobial peptides
Paenibacillus larvae
Tetracycline resistance
tet(45)
Péptidos antimicrobianos
Resistencia a tetraciclina
spellingShingle Biología
Bacillus thuringiensis sv. kumamotoensis
Antimicrobial peptides
Paenibacillus larvae
Tetracycline resistance
tet(45)
Péptidos antimicrobianos
Resistencia a tetraciclina
Alippi, Adriana Mónica
Lamelza, Florencia
Torres Tejerizo, Gonzalo Arturo
Abrahamovich, Eliana
López, Ana Claudia
Identification, phylogenetic analysis, and genome mining of the tetracycline-resistant Bacillus thuringiensis strain m401 reveal its potential for biotechnological and biocontrol applications
topic_facet Biología
Bacillus thuringiensis sv. kumamotoensis
Antimicrobial peptides
Paenibacillus larvae
Tetracycline resistance
tet(45)
Péptidos antimicrobianos
Resistencia a tetraciclina
description Bacillus thuringiensis is an entomopathogen belonging to the Bacillus cereus clade. We isolated a tetracycline-resistant strain called m401, recovered it from honey, and identified it as Bacillus thuringiensis sv. kumamotoensis based on the average nucleotide identity calculations (ANIb) comparison and the analysis of the gyrB gene sequences of different B. thuringiensis serovars. Sequences with homology to virulence factors [cytK, nheA, nheB, nheC, hblA, hblB, hblC, hblD, entFM, and inhA] and tetracycline resistance genes [tet(45), tet(V), and tet(M)/tet(W)/tet(O)/tet(S) family] were identified in the bacterial chromosome. The prediction of plasmid-coding regions revealed homolog sequences to the MarR and TetR/AcrR family of transcriptional regulators, toxins, and lantipeptides. The genome mining analysis revealed 12 regions of biosynthetic gene clusters responsible for synthesizing secondary metabolites. We identified biosynthetic gene clusters coding for bacteriocins, siderophores, ribosomally synthesized post-translationally modified peptide products, and non-ribosomal peptide synthetase clusters that provide evidence for the possible use of Bt m401 as a biocontrol agent. Furthermore, Bt m401 showed high inhibition against all Paenibacillus larvae genotypes tested in vitro. In conclusion, Bt m401 owns various genes involved in different biological processes, such as transductional regulators associated with antibiotic resistance, toxins, and antimicrobial peptides with potential biotechnological and biocontrol applications.
format Articulo
Articulo
author Alippi, Adriana Mónica
Lamelza, Florencia
Torres Tejerizo, Gonzalo Arturo
Abrahamovich, Eliana
López, Ana Claudia
author_facet Alippi, Adriana Mónica
Lamelza, Florencia
Torres Tejerizo, Gonzalo Arturo
Abrahamovich, Eliana
López, Ana Claudia
author_sort Alippi, Adriana Mónica
title Identification, phylogenetic analysis, and genome mining of the tetracycline-resistant Bacillus thuringiensis strain m401 reveal its potential for biotechnological and biocontrol applications
title_short Identification, phylogenetic analysis, and genome mining of the tetracycline-resistant Bacillus thuringiensis strain m401 reveal its potential for biotechnological and biocontrol applications
title_full Identification, phylogenetic analysis, and genome mining of the tetracycline-resistant Bacillus thuringiensis strain m401 reveal its potential for biotechnological and biocontrol applications
title_fullStr Identification, phylogenetic analysis, and genome mining of the tetracycline-resistant Bacillus thuringiensis strain m401 reveal its potential for biotechnological and biocontrol applications
title_full_unstemmed Identification, phylogenetic analysis, and genome mining of the tetracycline-resistant Bacillus thuringiensis strain m401 reveal its potential for biotechnological and biocontrol applications
title_sort identification, phylogenetic analysis, and genome mining of the tetracycline-resistant bacillus thuringiensis strain m401 reveal its potential for biotechnological and biocontrol applications
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/159941
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spelling I19-R120-10915-1599412023-11-08T20:03:49Z http://sedici.unlp.edu.ar/handle/10915/159941 Identification, phylogenetic analysis, and genome mining of the tetracycline-resistant Bacillus thuringiensis strain m401 reveal its potential for biotechnological and biocontrol applications Identificación, análisis filogenético y minería genómica de la cepa de Bacillus thuringiensis m401 resistente a tetraciclina, y sus posibles aplicaciones biotecnológicas y de biocontrol Alippi, Adriana Mónica Lamelza, Florencia Torres Tejerizo, Gonzalo Arturo Abrahamovich, Eliana López, Ana Claudia 2023 2023-11-08T15:23:21Z en Biología Bacillus thuringiensis sv. kumamotoensis Antimicrobial peptides Paenibacillus larvae Tetracycline resistance tet(45) Péptidos antimicrobianos Resistencia a tetraciclina Bacillus thuringiensis is an entomopathogen belonging to the Bacillus cereus clade. We isolated a tetracycline-resistant strain called m401, recovered it from honey, and identified it as Bacillus thuringiensis sv. kumamotoensis based on the average nucleotide identity calculations (ANIb) comparison and the analysis of the gyrB gene sequences of different B. thuringiensis serovars. Sequences with homology to virulence factors [cytK, nheA, nheB, nheC, hblA, hblB, hblC, hblD, entFM, and inhA] and tetracycline resistance genes [tet(45), tet(V), and tet(M)/tet(W)/tet(O)/tet(S) family] were identified in the bacterial chromosome. The prediction of plasmid-coding regions revealed homolog sequences to the MarR and TetR/AcrR family of transcriptional regulators, toxins, and lantipeptides. The genome mining analysis revealed 12 regions of biosynthetic gene clusters responsible for synthesizing secondary metabolites. We identified biosynthetic gene clusters coding for bacteriocins, siderophores, ribosomally synthesized post-translationally modified peptide products, and non-ribosomal peptide synthetase clusters that provide evidence for the possible use of Bt m401 as a biocontrol agent. Furthermore, Bt m401 showed high inhibition against all Paenibacillus larvae genotypes tested in vitro. In conclusion, Bt m401 owns various genes involved in different biological processes, such as transductional regulators associated with antibiotic resistance, toxins, and antimicrobial peptides with potential biotechnological and biocontrol applications. Bacillus thuringiensis es un microorganismo entomopatógeno perteneciente al clado Bacillus cereus. En este trabajo, aislamos de miel una cepa resistente a tetraciclina y la identificamos como Bacillus thuringiensis sv. kumamotoensis (Bt m401) mediante comparación de genomas completos y cálculo de identidad promedio de nucleótidos (ANIb), utilizando la base de datos EDGAR. Este resultado fue corroborado por el análisis filogenético del gen gyrB, que permite diferenciar serovares de B. thuringiensis. Se confirmó la presencia del gen de resistencia a tetraciclina tet(45) en el cromosoma bacteriano y se identificaron secuencias homólogas a genes de virulencia (cytK, nheA, nheB, nheC, hblA, hblB, hblC, hblD y entFM). El mobiloma de Bt m401 está constituido por 4 plásmidos y se identificaron secuencias homólogas a reguladores transduccionales (MarR y TetR/AcR), a toxinas (Cry1 y zeta toxin) y a péptidos antimicrobianos (mersacidin family lantibiotics). La cepa Bt m401 demostró una muy buena actividad antagónica in vitro contra distintos genotipos de Paenibacillus larvae, agente causal de la loque americana de las abejas. El estudio de minería genómica para grupos de genes biosintéticos reveló 12 regiones de clústeres de genes responsables de la síntesis de metabolitos secundarios. Se identificaron clústeres que codifican bacteriocinas, sideróforos, RiPP-like y NRPS y lantipéptidos. En conclusión, Bt m401 presentó una amplia variedad de genes que estarían involucrados en diferentes procesos biológicos, con un potencial interesante para su empleo en aplicaciones biotecnológicas. Centro de Investigaciones en Fitopatología Instituto de Biotecnología y Biología Molecular Articulo Articulo http://creativecommons.org/licenses/by-nc-nd/4.0/ Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) application/pdf

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