Characterization of a Clostridioides difficile ST-293 isolate from a recurrent infection in Argentina

Clostridioides difficile is an opportunistic spore-forming pathogen responsible for antibiotic-associated diarrhea in humans. C. difficile produces two main toxins: TcdA and TcdB as well as a third toxin named binary toxin (CDT) that is also involved in virulence. The present study aimed at characte...

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Autores principales: Soldavini Pelichotti, Paola Cecilia, Cejas, Daniela, Fernández Caniggia, Liliana, Trejo, Fernando Miguel, Pérez, Pablo Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Biología
Clostridioides difficile
Pathogenesis
Sporulation
Germination
Recurrence
MLST
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/159853
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-159853
record_format dspace
spelling I19-R120-10915-1598532023-11-08T20:07:37Z http://sedici.unlp.edu.ar/handle/10915/159853 Characterization of a Clostridioides difficile ST-293 isolate from a recurrent infection in Argentina Soldavini Pelichotti, Paola Cecilia Cejas, Daniela Fernández Caniggia, Liliana Trejo, Fernando Miguel Pérez, Pablo Fernando 2023 2023-11-06T19:07:17Z en Biología Clostridioides difficile Pathogenesis Sporulation Germination Recurrence MLST Clostridioides difficile is an opportunistic spore-forming pathogen responsible for antibiotic-associated diarrhea in humans. C. difficile produces two main toxins: TcdA and TcdB as well as a third toxin named binary toxin (CDT) that is also involved in virulence. The present study aimed at characterizing the C. difficile isolate ALCD3 involved in a relapse episode of nosocomial infection. Molecular characterization showed that isolate ALCD3 belongs to toxinotype 0/v and the MLST analysis demonstrated allelic profile adk:91, atpA:1, dxr:2, glyA: 1, recA:27, sodA: 1 and tpi:1 which corresponds to ST293 (MLST clade: 1). During growth, isolate ALCD3 showed an early increase in the sporulation ratio as well as maximal values of heat resistant forms after 2 days of incubation. Both sporulation kinetics and production of heat resistant forms were faster for isolate ALCD3 than for the reference strain VPI 10463. Germination in the presence of the natural germinant taurocholate was faster for isolate ALCD3 than for strain VPI 10463, which indicates that isolate ALCD3 starts cortex hydrolysis earlier than strain VPI 10463. Furthermore, the co-germinant glycine, induces rapid release of dipicolinic acid (DPA) in isolate ALCD3. These findings indicate that isolate ALCD3 is particularly efficient in both sporulation and germination. The present work represents the first report of the circulation of C. difficile ST293 in Argentina. The ability of isolate ALCD3 to produce toxins and its high sporulation/germination capacity are key features compatible with a microorganism with high dissemination potential and the possibility of inducing recurrent infections. Centro de Investigación y Desarrollo en Criotecnología de Alimentos Articulo Articulo http://creativecommons.org/licenses/by-nc-nd/4.0/ Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) application/pdf 150-159
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Clostridioides difficile
Pathogenesis
Sporulation
Germination
Recurrence
MLST
spellingShingle Biología
Clostridioides difficile
Pathogenesis
Sporulation
Germination
Recurrence
MLST
Soldavini Pelichotti, Paola Cecilia
Cejas, Daniela
Fernández Caniggia, Liliana
Trejo, Fernando Miguel
Pérez, Pablo Fernando
Characterization of a Clostridioides difficile ST-293 isolate from a recurrent infection in Argentina
topic_facet Biología
Clostridioides difficile
Pathogenesis
Sporulation
Germination
Recurrence
MLST
description Clostridioides difficile is an opportunistic spore-forming pathogen responsible for antibiotic-associated diarrhea in humans. C. difficile produces two main toxins: TcdA and TcdB as well as a third toxin named binary toxin (CDT) that is also involved in virulence. The present study aimed at characterizing the C. difficile isolate ALCD3 involved in a relapse episode of nosocomial infection. Molecular characterization showed that isolate ALCD3 belongs to toxinotype 0/v and the MLST analysis demonstrated allelic profile adk:91, atpA:1, dxr:2, glyA: 1, recA:27, sodA: 1 and tpi:1 which corresponds to ST293 (MLST clade: 1). During growth, isolate ALCD3 showed an early increase in the sporulation ratio as well as maximal values of heat resistant forms after 2 days of incubation. Both sporulation kinetics and production of heat resistant forms were faster for isolate ALCD3 than for the reference strain VPI 10463. Germination in the presence of the natural germinant taurocholate was faster for isolate ALCD3 than for strain VPI 10463, which indicates that isolate ALCD3 starts cortex hydrolysis earlier than strain VPI 10463. Furthermore, the co-germinant glycine, induces rapid release of dipicolinic acid (DPA) in isolate ALCD3. These findings indicate that isolate ALCD3 is particularly efficient in both sporulation and germination. The present work represents the first report of the circulation of C. difficile ST293 in Argentina. The ability of isolate ALCD3 to produce toxins and its high sporulation/germination capacity are key features compatible with a microorganism with high dissemination potential and the possibility of inducing recurrent infections.
format Articulo
Articulo
author Soldavini Pelichotti, Paola Cecilia
Cejas, Daniela
Fernández Caniggia, Liliana
Trejo, Fernando Miguel
Pérez, Pablo Fernando
author_facet Soldavini Pelichotti, Paola Cecilia
Cejas, Daniela
Fernández Caniggia, Liliana
Trejo, Fernando Miguel
Pérez, Pablo Fernando
author_sort Soldavini Pelichotti, Paola Cecilia
title Characterization of a Clostridioides difficile ST-293 isolate from a recurrent infection in Argentina
title_short Characterization of a Clostridioides difficile ST-293 isolate from a recurrent infection in Argentina
title_full Characterization of a Clostridioides difficile ST-293 isolate from a recurrent infection in Argentina
title_fullStr Characterization of a Clostridioides difficile ST-293 isolate from a recurrent infection in Argentina
title_full_unstemmed Characterization of a Clostridioides difficile ST-293 isolate from a recurrent infection in Argentina
title_sort characterization of a clostridioides difficile st-293 isolate from a recurrent infection in argentina
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/159853
work_keys_str_mv AT soldavinipelichottipaolacecilia characterizationofaclostridioidesdifficilest293isolatefromarecurrentinfectioninargentina
AT cejasdaniela characterizationofaclostridioidesdifficilest293isolatefromarecurrentinfectioninargentina
AT fernandezcaniggialiliana characterizationofaclostridioidesdifficilest293isolatefromarecurrentinfectioninargentina
AT trejofernandomiguel characterizationofaclostridioidesdifficilest293isolatefromarecurrentinfectioninargentina
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