Cytogenetic study in the Brazilian semiarid lizard Tropidurus hispidus (Squamata, Tropiduridae)

Different classes of repetitive DNA sequences are found in eukaryotes, often composing substantial portions of the genome, associated with their important role in the structural and functional genome organization. In this work, we mapped repetitive DNA sequences (18S rDNA, microsatellites and telome...

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Autores principales: da Silva, Marcelo João, de Araújo Vieira, Ana Paula, Galvão Cipriano, Flávia Manoela, dos Santos Cândido, Maria Rita, Gimenez Pinheiro, Tamaris, Correa Oliveira, Edivaldo Herculano, Lourenço da Silva, Edson
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Zoología
Reptile chromosomes
Microsatellite
18S rDNA
Telomeric repeats
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/156736
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SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-156736
record_format dspace
spelling I19-R120-10915-1567362023-08-23T20:02:59Z http://sedici.unlp.edu.ar/handle/10915/156736 Cytogenetic study in the Brazilian semiarid lizard Tropidurus hispidus (Squamata, Tropiduridae) da Silva, Marcelo João de Araújo Vieira, Ana Paula Galvão Cipriano, Flávia Manoela dos Santos Cândido, Maria Rita Gimenez Pinheiro, Tamaris Correa Oliveira, Edivaldo Herculano Lourenço da Silva, Edson 2023-05 2023-08-23T16:51:55Z en Zoología Reptile chromosomes Microsatellite 18S rDNA Telomeric repeats Different classes of repetitive DNA sequences are found in eukaryotes, often composing substantial portions of the genome, associated with their important role in the structural and functional genome organization. In this work, we mapped repetitive DNA sequences (18S rDNA, microsatellites and telomeric motifs) in the karyotype of Tropidurus hispidus, a species of lizard from the Brazilian semiarid region. We found a diploid number of 2n = 36 (6 pairs of biarmed macrochromosomes and 12 pairs of microchromosomes). The 18S rDNA clusters were localized at the subterminal region of the long arm of pair 2. The telomeric probes produced signals at terminal, interstitial and centromeric positions of some chromosome pairs, which might indicate the occurrence of chromosomal rearrangements via chromosome fusions. Microsatellite sequences were found in at least two distinct patterns - clustered in the telomeric/pericentromeric regions or observed as scattered signals in the chromosomes. This study represents an initial step to explore the evolutionary dynamics of repetitive sequences in the Tropidurus genus and considering the scarcity of data concerning the chromosomal mapping of repetitive sequences in Tropiduridae species, it reinforces the importance of integrating other methodologies, including the isolation and physical mapping of different repetitive DNA sequences, contributing to understanding the patterns of karyotypic evolution in lizards. Asociación Herpetológica Argentina Articulo Articulo http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) application/pdf 57-64
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Zoología
Reptile chromosomes
Microsatellite
18S rDNA
Telomeric repeats
spellingShingle Zoología
Reptile chromosomes
Microsatellite
18S rDNA
Telomeric repeats
da Silva, Marcelo João
de Araújo Vieira, Ana Paula
Galvão Cipriano, Flávia Manoela
dos Santos Cândido, Maria Rita
Gimenez Pinheiro, Tamaris
Correa Oliveira, Edivaldo Herculano
Lourenço da Silva, Edson
Cytogenetic study in the Brazilian semiarid lizard Tropidurus hispidus (Squamata, Tropiduridae)
topic_facet Zoología
Reptile chromosomes
Microsatellite
18S rDNA
Telomeric repeats
description Different classes of repetitive DNA sequences are found in eukaryotes, often composing substantial portions of the genome, associated with their important role in the structural and functional genome organization. In this work, we mapped repetitive DNA sequences (18S rDNA, microsatellites and telomeric motifs) in the karyotype of Tropidurus hispidus, a species of lizard from the Brazilian semiarid region. We found a diploid number of 2n = 36 (6 pairs of biarmed macrochromosomes and 12 pairs of microchromosomes). The 18S rDNA clusters were localized at the subterminal region of the long arm of pair 2. The telomeric probes produced signals at terminal, interstitial and centromeric positions of some chromosome pairs, which might indicate the occurrence of chromosomal rearrangements via chromosome fusions. Microsatellite sequences were found in at least two distinct patterns - clustered in the telomeric/pericentromeric regions or observed as scattered signals in the chromosomes. This study represents an initial step to explore the evolutionary dynamics of repetitive sequences in the Tropidurus genus and considering the scarcity of data concerning the chromosomal mapping of repetitive sequences in Tropiduridae species, it reinforces the importance of integrating other methodologies, including the isolation and physical mapping of different repetitive DNA sequences, contributing to understanding the patterns of karyotypic evolution in lizards.
format Articulo
Articulo
author da Silva, Marcelo João
de Araújo Vieira, Ana Paula
Galvão Cipriano, Flávia Manoela
dos Santos Cândido, Maria Rita
Gimenez Pinheiro, Tamaris
Correa Oliveira, Edivaldo Herculano
Lourenço da Silva, Edson
author_facet da Silva, Marcelo João
de Araújo Vieira, Ana Paula
Galvão Cipriano, Flávia Manoela
dos Santos Cândido, Maria Rita
Gimenez Pinheiro, Tamaris
Correa Oliveira, Edivaldo Herculano
Lourenço da Silva, Edson
author_sort da Silva, Marcelo João
title Cytogenetic study in the Brazilian semiarid lizard Tropidurus hispidus (Squamata, Tropiduridae)
title_short Cytogenetic study in the Brazilian semiarid lizard Tropidurus hispidus (Squamata, Tropiduridae)
title_full Cytogenetic study in the Brazilian semiarid lizard Tropidurus hispidus (Squamata, Tropiduridae)
title_fullStr Cytogenetic study in the Brazilian semiarid lizard Tropidurus hispidus (Squamata, Tropiduridae)
title_full_unstemmed Cytogenetic study in the Brazilian semiarid lizard Tropidurus hispidus (Squamata, Tropiduridae)
title_sort cytogenetic study in the brazilian semiarid lizard tropidurus hispidus (squamata, tropiduridae)
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/156736
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