Molecular detection of Bartonella in fleas (Hexapoda, Siphonaptera) collected from wild rodents (Cricetidae, Sigmodontinae) from Argentina

Bartonella are facultative intracellular Gram-negative bacteria, transmitted mainly by hematophagous arthropods, and the rodents act as a natural reservoir. Different species of Bartonella associated with rodents have been implicated as causing human disease. Studies from Argentina are scarce and no...

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Detalles Bibliográficos
Autores principales: Cicuttin, Gabriel Leonardo, De Salvo, María Nazarena, Sánchez, J., Cañón, C., Lareschi, Marcela
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2019
Materias:
Ciencias Naturales
Bartonella
Siphonaptera
Bacteria
fleas
rodent
Argentina
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/156603
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-156603
record_format dspace
spelling I19-R120-10915-1566032023-08-19T20:06:59Z http://sedici.unlp.edu.ar/handle/10915/156603 Molecular detection of Bartonella in fleas (Hexapoda, Siphonaptera) collected from wild rodents (Cricetidae, Sigmodontinae) from Argentina Cicuttin, Gabriel Leonardo De Salvo, María Nazarena Sánchez, J. Cañón, C. Lareschi, Marcela 2019 2023-08-18T17:35:42Z en Ciencias Naturales Bartonella Siphonaptera Bacteria fleas rodent Argentina Bartonella are facultative intracellular Gram-negative bacteria, transmitted mainly by hematophagous arthropods, and the rodents act as a natural reservoir. Different species of Bartonella associated with rodents have been implicated as causing human disease. Studies from Argentina are scarce and no Bartonella from fleas have been reported previously. The present study investigated the presence of Bartonella spp. in fleas associated with sigmodontine rodents in four localities of the Santa Cruz Province, Argentina. In total, 51 fleas (four species) were analysed of which 41.2% were found to be positive for the gltA gene fragment via a nested polymerase chain reaction. All positive fleas were of the species Neotyphloceras crackensis from three different localities. Eight of the 21 amplified samples were sequenced, and the presence of three different genotypes was detected with an identity of 95.5–98.8% amongst themselves. Bartonella genotypes from American rodents and rodent fleas were recovered in a monophyletic group. Similarly, most of the Peruvian and all Argentinean variants constitute a natural group sister of the American remainder. The importance of the Bartonella spp. with respect to public health is unknown, although future studies could provide evidence of the possible involvement of N. crackensis in the Bartonella transmission cycles. Centro de Estudios Parasitológicos y de Vectores Consejo Nacional de Investigaciones Científicas y Técnicas Articulo Comunicacion http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 541-545
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Bartonella
Siphonaptera
Bacteria
fleas
rodent
Argentina
spellingShingle Ciencias Naturales
Bartonella
Siphonaptera
Bacteria
fleas
rodent
Argentina
Cicuttin, Gabriel Leonardo
De Salvo, María Nazarena
Sánchez, J.
Cañón, C.
Lareschi, Marcela
Molecular detection of Bartonella in fleas (Hexapoda, Siphonaptera) collected from wild rodents (Cricetidae, Sigmodontinae) from Argentina
topic_facet Ciencias Naturales
Bartonella
Siphonaptera
Bacteria
fleas
rodent
Argentina
description Bartonella are facultative intracellular Gram-negative bacteria, transmitted mainly by hematophagous arthropods, and the rodents act as a natural reservoir. Different species of Bartonella associated with rodents have been implicated as causing human disease. Studies from Argentina are scarce and no Bartonella from fleas have been reported previously. The present study investigated the presence of Bartonella spp. in fleas associated with sigmodontine rodents in four localities of the Santa Cruz Province, Argentina. In total, 51 fleas (four species) were analysed of which 41.2% were found to be positive for the gltA gene fragment via a nested polymerase chain reaction. All positive fleas were of the species Neotyphloceras crackensis from three different localities. Eight of the 21 amplified samples were sequenced, and the presence of three different genotypes was detected with an identity of 95.5–98.8% amongst themselves. Bartonella genotypes from American rodents and rodent fleas were recovered in a monophyletic group. Similarly, most of the Peruvian and all Argentinean variants constitute a natural group sister of the American remainder. The importance of the Bartonella spp. with respect to public health is unknown, although future studies could provide evidence of the possible involvement of N. crackensis in the Bartonella transmission cycles.
format Articulo
Comunicacion
author Cicuttin, Gabriel Leonardo
De Salvo, María Nazarena
Sánchez, J.
Cañón, C.
Lareschi, Marcela
author_facet Cicuttin, Gabriel Leonardo
De Salvo, María Nazarena
Sánchez, J.
Cañón, C.
Lareschi, Marcela
author_sort Cicuttin, Gabriel Leonardo
title Molecular detection of Bartonella in fleas (Hexapoda, Siphonaptera) collected from wild rodents (Cricetidae, Sigmodontinae) from Argentina
title_short Molecular detection of Bartonella in fleas (Hexapoda, Siphonaptera) collected from wild rodents (Cricetidae, Sigmodontinae) from Argentina
title_full Molecular detection of Bartonella in fleas (Hexapoda, Siphonaptera) collected from wild rodents (Cricetidae, Sigmodontinae) from Argentina
title_fullStr Molecular detection of Bartonella in fleas (Hexapoda, Siphonaptera) collected from wild rodents (Cricetidae, Sigmodontinae) from Argentina
title_full_unstemmed Molecular detection of Bartonella in fleas (Hexapoda, Siphonaptera) collected from wild rodents (Cricetidae, Sigmodontinae) from Argentina
title_sort molecular detection of bartonella in fleas (hexapoda, siphonaptera) collected from wild rodents (cricetidae, sigmodontinae) from argentina
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/156603
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