Analysis of the Metabolic Response of Planktonic Cells and Biofilms of Klebsiella pneumoniae to Sublethal Disinfection with Sodium Hypochlorite Measured by NMR

Klebsiella pneumoniae is a pathogenic agent able to form biofilms on water storage tanks and pipe walls. This opportunistic pathogen can generate a thick layer as one of its essential virulence factors, enabling the bacteria to survive disinfection processes and thus develop drug resistance. Unders...

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Detalles Bibliográficos
Autores principales: García Méndez, David Felipe, Rengifo Herrera, Julián Andrés, Sanabria, Janeth, Wist, Julien
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Biología
exometabolome
metabolism
metabolite
chlorine
chlorination
NaOCl
Klebsiella
water
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/156305
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-156305
record_format dspace
spelling I19-R120-10915-1563052023-08-14T20:08:17Z http://sedici.unlp.edu.ar/handle/10915/156305 Analysis of the Metabolic Response of Planktonic Cells and Biofilms of Klebsiella pneumoniae to Sublethal Disinfection with Sodium Hypochlorite Measured by NMR García Méndez, David Felipe Rengifo Herrera, Julián Andrés Sanabria, Janeth Wist, Julien 2022 2023-08-14T18:16:02Z en Biología exometabolome metabolism metabolite chlorine chlorination NaOCl Klebsiella water Klebsiella pneumoniae is a pathogenic agent able to form biofilms on water storage tanks and pipe walls. This opportunistic pathogen can generate a thick layer as one of its essential virulence factors, enabling the bacteria to survive disinfection processes and thus develop drug resistance. Understanding the metabolic differences between biofilm and planktonic cells of the K. pneumoniae response to NaClO is key to developing strategies to control its spread. In this study, we performed an NMR metabolic profile analysis to compare the response to a sublethal concentration of sodium hypochlorite of biofilm and planktonic cells of K. pneumoniae cultured inside silicone tubing. Metabolic profiles revealed changes in the metabolism of planktonic cells after a contact time of 10 min with 7 mg L−1 of sodium hypochlorite. A decrease in the production of metabolites such as lactate, acetate, ethanol, and succinate in this cell type was observed, thus indicating a disruption of glucose intake. In contrast, the biofilms displayed a high metabolic heterogeneity, and the treatment did not affect their metabolic signature. Centro de Investigación y Desarrollo en Ciencias Aplicadas Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
exometabolome
metabolism
metabolite
chlorine
chlorination
NaOCl
Klebsiella
water
spellingShingle Biología
exometabolome
metabolism
metabolite
chlorine
chlorination
NaOCl
Klebsiella
water
García Méndez, David Felipe
Rengifo Herrera, Julián Andrés
Sanabria, Janeth
Wist, Julien
Analysis of the Metabolic Response of Planktonic Cells and Biofilms of Klebsiella pneumoniae to Sublethal Disinfection with Sodium Hypochlorite Measured by NMR
topic_facet Biología
exometabolome
metabolism
metabolite
chlorine
chlorination
NaOCl
Klebsiella
water
description Klebsiella pneumoniae is a pathogenic agent able to form biofilms on water storage tanks and pipe walls. This opportunistic pathogen can generate a thick layer as one of its essential virulence factors, enabling the bacteria to survive disinfection processes and thus develop drug resistance. Understanding the metabolic differences between biofilm and planktonic cells of the K. pneumoniae response to NaClO is key to developing strategies to control its spread. In this study, we performed an NMR metabolic profile analysis to compare the response to a sublethal concentration of sodium hypochlorite of biofilm and planktonic cells of K. pneumoniae cultured inside silicone tubing. Metabolic profiles revealed changes in the metabolism of planktonic cells after a contact time of 10 min with 7 mg L−1 of sodium hypochlorite. A decrease in the production of metabolites such as lactate, acetate, ethanol, and succinate in this cell type was observed, thus indicating a disruption of glucose intake. In contrast, the biofilms displayed a high metabolic heterogeneity, and the treatment did not affect their metabolic signature.
format Articulo
Articulo
author García Méndez, David Felipe
Rengifo Herrera, Julián Andrés
Sanabria, Janeth
Wist, Julien
author_facet García Méndez, David Felipe
Rengifo Herrera, Julián Andrés
Sanabria, Janeth
Wist, Julien
author_sort García Méndez, David Felipe
title Analysis of the Metabolic Response of Planktonic Cells and Biofilms of Klebsiella pneumoniae to Sublethal Disinfection with Sodium Hypochlorite Measured by NMR
title_short Analysis of the Metabolic Response of Planktonic Cells and Biofilms of Klebsiella pneumoniae to Sublethal Disinfection with Sodium Hypochlorite Measured by NMR
title_full Analysis of the Metabolic Response of Planktonic Cells and Biofilms of Klebsiella pneumoniae to Sublethal Disinfection with Sodium Hypochlorite Measured by NMR
title_fullStr Analysis of the Metabolic Response of Planktonic Cells and Biofilms of Klebsiella pneumoniae to Sublethal Disinfection with Sodium Hypochlorite Measured by NMR
title_full_unstemmed Analysis of the Metabolic Response of Planktonic Cells and Biofilms of Klebsiella pneumoniae to Sublethal Disinfection with Sodium Hypochlorite Measured by NMR
title_sort analysis of the metabolic response of planktonic cells and biofilms of klebsiella pneumoniae to sublethal disinfection with sodium hypochlorite measured by nmr
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/156305
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