The magic staff: a comprehensive overview of baculovirus-based technologies applied to human and animal health

Baculoviruses are enveloped, insect-specific viruses with large double-stranded DNA genomes. Among all the baculovirus species, Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the most studied. Due to its characteristics regarding biosafety, narrow host range and the availability of...

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Autores principales: Pidre, Matías Luis, Arrías, Paula Nazarena, Amorós Morales, Leslie Cinthya, Romanowski, Víctor
Formato: Articulo Revision
Lenguaje:Inglés
Publicado: 2022
Materias:
Biología
Baculovirus
BEVS
BacMam
Viral vectors
Vaccines
Gene therapy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/154822
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-154822
record_format dspace
spelling I19-R120-10915-1548222023-06-30T04:06:52Z http://sedici.unlp.edu.ar/handle/10915/154822 issn:1999-4915 The magic staff: a comprehensive overview of baculovirus-based technologies applied to human and animal health Pidre, Matías Luis Arrías, Paula Nazarena Amorós Morales, Leslie Cinthya Romanowski, Víctor 2022 2023-06-29T16:44:12Z en Biología Baculovirus BEVS BacMam Viral vectors Vaccines Gene therapy Baculoviruses are enveloped, insect-specific viruses with large double-stranded DNA genomes. Among all the baculovirus species, Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the most studied. Due to its characteristics regarding biosafety, narrow host range and the availability of different platforms for modifying its genome, AcMNPV has become a powerful biotechnological tool. In this review, we will address the most widespread technological applications of baculoviruses. We will begin by summarizing their natural cycle both in larvae and in cell culture and how it can be exploited. Secondly, we will explore the different baculovirus-based protein expression systems (BEVS) and their multiple applications in the pharmaceutical and biotechnological industry. We will focus particularly on the production of vaccines, many of which are either currently commercialized or in advanced stages of development (e.g., Novavax, COVID-19 vaccine). In addition, recombinant baculoviruses can be used as efficient gene transduction and protein expression vectors in vertebrate cells (e.g., BacMam). Finally, we will extensively describe various gene therapy strategies based on baculoviruses applied to the treatment of different diseases. The main objective of this work is to provide an extensive up-to-date summary of the different biotechnological applications of baculoviruses, emphasizing the genetic modification strategies used in each field. Instituto de Biotecnología y Biología Molecular Articulo Revision http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Baculovirus
BEVS
BacMam
Viral vectors
Vaccines
Gene therapy
spellingShingle Biología
Baculovirus
BEVS
BacMam
Viral vectors
Vaccines
Gene therapy
Pidre, Matías Luis
Arrías, Paula Nazarena
Amorós Morales, Leslie Cinthya
Romanowski, Víctor
The magic staff: a comprehensive overview of baculovirus-based technologies applied to human and animal health
topic_facet Biología
Baculovirus
BEVS
BacMam
Viral vectors
Vaccines
Gene therapy
description Baculoviruses are enveloped, insect-specific viruses with large double-stranded DNA genomes. Among all the baculovirus species, Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the most studied. Due to its characteristics regarding biosafety, narrow host range and the availability of different platforms for modifying its genome, AcMNPV has become a powerful biotechnological tool. In this review, we will address the most widespread technological applications of baculoviruses. We will begin by summarizing their natural cycle both in larvae and in cell culture and how it can be exploited. Secondly, we will explore the different baculovirus-based protein expression systems (BEVS) and their multiple applications in the pharmaceutical and biotechnological industry. We will focus particularly on the production of vaccines, many of which are either currently commercialized or in advanced stages of development (e.g., Novavax, COVID-19 vaccine). In addition, recombinant baculoviruses can be used as efficient gene transduction and protein expression vectors in vertebrate cells (e.g., BacMam). Finally, we will extensively describe various gene therapy strategies based on baculoviruses applied to the treatment of different diseases. The main objective of this work is to provide an extensive up-to-date summary of the different biotechnological applications of baculoviruses, emphasizing the genetic modification strategies used in each field.
format Articulo
Revision
author Pidre, Matías Luis
Arrías, Paula Nazarena
Amorós Morales, Leslie Cinthya
Romanowski, Víctor
author_facet Pidre, Matías Luis
Arrías, Paula Nazarena
Amorós Morales, Leslie Cinthya
Romanowski, Víctor
author_sort Pidre, Matías Luis
title The magic staff: a comprehensive overview of baculovirus-based technologies applied to human and animal health
title_short The magic staff: a comprehensive overview of baculovirus-based technologies applied to human and animal health
title_full The magic staff: a comprehensive overview of baculovirus-based technologies applied to human and animal health
title_fullStr The magic staff: a comprehensive overview of baculovirus-based technologies applied to human and animal health
title_full_unstemmed The magic staff: a comprehensive overview of baculovirus-based technologies applied to human and animal health
title_sort magic staff: a comprehensive overview of baculovirus-based technologies applied to human and animal health
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/154822
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