Antarctic yeasts: potential use in a biologic treatment of textile azo dyes

We investigated the dye-removal potential of a collection of 61 cold-adapted yeasts from the King George Island, Antarctica, on agar plates supplemented with 100 mg L⁻¹ of several textile dyes; among which isolates 81% decolorized Reactive Black 5 (RB-5), with 56% decolorizing Reactive Orange 16, bu...

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Autores principales: Ruscasso, María Florencia, Cavello, Ivana Alejandra, Curutchet, Gustavo Andrés, Cavalitto, Sebastián Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Bioquímica
Leucosporidium muscorum F20A
Cold-adapted yeasts
Textile wastewaters
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/154820
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-154820
record_format dspace
spelling I19-R120-10915-1548202023-06-30T04:07:04Z http://sedici.unlp.edu.ar/handle/10915/154820 issn:2197-4365 Antarctic yeasts: potential use in a biologic treatment of textile azo dyes Ruscasso, María Florencia Cavello, Ivana Alejandra Curutchet, Gustavo Andrés Cavalitto, Sebastián Fernando 2022 2023-06-29T16:27:30Z en Bioquímica Leucosporidium muscorum F20A Cold-adapted yeasts Textile wastewaters We investigated the dye-removal potential of a collection of 61 cold-adapted yeasts from the King George Island, Antarctica, on agar plates supplemented with 100 mg L⁻¹ of several textile dyes; among which isolates 81% decolorized Reactive Black 5 (RB-5), with 56% decolorizing Reactive Orange 16, but only 26% doing so with Reactive Blue 19 and Acid Blue 74. Furthermore, we evaluated the ligninolytic potential using 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6- sulfonic-acid) diammonium salt-, 3,5-dimethoxy-4-hydroxybenzaldehydazine-, or manganese-supplemented plates but detected no activity, possibly due to a dye-removal mechanism involving reductases. The removal kinetics were studied in liquid medium supplemented with 100 mg L⁻¹ of RB-5 in a selection of 9 yeasts. The highest volumetricremoval rates (η) were found for Candida sake 41E (4.14 mg L⁻¹ h⁻¹), Leucosporidium muscorum F20A (3.90 mg L⁻¹ h⁻¹), and Cystofilobasidium infirmominiatum F13E (3.90 mg L⁻¹ h⁻¹). Different UV–Vis spectra were obtained if the dye removal occurred by biodegradation or biosorption/bioaccumulation. L. muscorum F20A was selected to study the dye-removal mechanism of RB-5 and the effect of different chemical and environmental parameters on the process. Optimum dye-removal conditions were obtained with 10 g L⁻¹ of glucose within an initial medium pH range of 5.0 to 6.0. Up to 700 mg ⁻¹ of dye could be removed in 45 h. High-performance liquid chromatography profiles obtained were consistent with a biodegradation of the dye. Phytotoxicity was estimated by calculating the 50%-inhibition concentration ( IC50) with Lactuca sativa L. seeds. These findings propose psychrophilic yeasts as a novel environmentally suitable alternative for the treatment of dye-industry wastewaters. Centro de Investigación y Desarrollo en Fermentaciones Industriales Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Leucosporidium muscorum F20A
Cold-adapted yeasts
Textile wastewaters
spellingShingle Bioquímica
Leucosporidium muscorum F20A
Cold-adapted yeasts
Textile wastewaters
Ruscasso, María Florencia
Cavello, Ivana Alejandra
Curutchet, Gustavo Andrés
Cavalitto, Sebastián Fernando
Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
topic_facet Bioquímica
Leucosporidium muscorum F20A
Cold-adapted yeasts
Textile wastewaters
description We investigated the dye-removal potential of a collection of 61 cold-adapted yeasts from the King George Island, Antarctica, on agar plates supplemented with 100 mg L⁻¹ of several textile dyes; among which isolates 81% decolorized Reactive Black 5 (RB-5), with 56% decolorizing Reactive Orange 16, but only 26% doing so with Reactive Blue 19 and Acid Blue 74. Furthermore, we evaluated the ligninolytic potential using 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6- sulfonic-acid) diammonium salt-, 3,5-dimethoxy-4-hydroxybenzaldehydazine-, or manganese-supplemented plates but detected no activity, possibly due to a dye-removal mechanism involving reductases. The removal kinetics were studied in liquid medium supplemented with 100 mg L⁻¹ of RB-5 in a selection of 9 yeasts. The highest volumetricremoval rates (η) were found for Candida sake 41E (4.14 mg L⁻¹ h⁻¹), Leucosporidium muscorum F20A (3.90 mg L⁻¹ h⁻¹), and Cystofilobasidium infirmominiatum F13E (3.90 mg L⁻¹ h⁻¹). Different UV–Vis spectra were obtained if the dye removal occurred by biodegradation or biosorption/bioaccumulation. L. muscorum F20A was selected to study the dye-removal mechanism of RB-5 and the effect of different chemical and environmental parameters on the process. Optimum dye-removal conditions were obtained with 10 g L⁻¹ of glucose within an initial medium pH range of 5.0 to 6.0. Up to 700 mg ⁻¹ of dye could be removed in 45 h. High-performance liquid chromatography profiles obtained were consistent with a biodegradation of the dye. Phytotoxicity was estimated by calculating the 50%-inhibition concentration ( IC50) with Lactuca sativa L. seeds. These findings propose psychrophilic yeasts as a novel environmentally suitable alternative for the treatment of dye-industry wastewaters.
format Articulo
Articulo
author Ruscasso, María Florencia
Cavello, Ivana Alejandra
Curutchet, Gustavo Andrés
Cavalitto, Sebastián Fernando
author_facet Ruscasso, María Florencia
Cavello, Ivana Alejandra
Curutchet, Gustavo Andrés
Cavalitto, Sebastián Fernando
author_sort Ruscasso, María Florencia
title Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
title_short Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
title_full Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
title_fullStr Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
title_full_unstemmed Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
title_sort antarctic yeasts: potential use in a biologic treatment of textile azo dyes
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/154820
work_keys_str_mv AT ruscassomariaflorencia antarcticyeastspotentialuseinabiologictreatmentoftextileazodyes
AT cavelloivanaalejandra antarcticyeastspotentialuseinabiologictreatmentoftextileazodyes
AT curutchetgustavoandres antarcticyeastspotentialuseinabiologictreatmentoftextileazodyes
AT cavalittosebastianfernando antarcticyeastspotentialuseinabiologictreatmentoftextileazodyes
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