Variability of PDYN and OPRK1 genes in four Argentinian populations and its genetic association with clinical variables related to acute postsurgical pain
Several population studies showed an association between variation in pain sensitivity and genetic polymorphisms located in Prodynorphin (PDYN) and Kappa Opioid Receptor (OPRK1) human genes. We analysed polymorphisms of these two genes to characterise their variation in Argentinian populations, as w...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2022
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/154324 |
| Aporte de: |
| id |
I19-R120-10915-154324 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Biología human populations pain sensitivity acute pain genetic polymorphisms genetic structure poblaciones humanas sensibilidad al dolor dolor agudo polimorfismos genéticos estructura genética |
| spellingShingle |
Ciencias Exactas Biología human populations pain sensitivity acute pain genetic polymorphisms genetic structure poblaciones humanas sensibilidad al dolor dolor agudo polimorfismos genéticos estructura genética Di Santo Meztler, Gabriela Paula Schiaffi, Jorge Rigalli, Alfredo Esteban Torné, María Esther Martina, Pablo Francisco Catanesi, Cecilia Inés Variability of PDYN and OPRK1 genes in four Argentinian populations and its genetic association with clinical variables related to acute postsurgical pain |
| topic_facet |
Ciencias Exactas Biología human populations pain sensitivity acute pain genetic polymorphisms genetic structure poblaciones humanas sensibilidad al dolor dolor agudo polimorfismos genéticos estructura genética |
| description |
Several population studies showed an association between variation in pain sensitivity and genetic polymorphisms located in Prodynorphin (PDYN) and Kappa Opioid Receptor (OPRK1) human genes. We analysed polymorphisms of these two genes to characterise their variation in Argentinian populations, as well as to evaluate their association with acute pain sensitivity. We studied 11 genetic markers in individuals from four locations in Argentina (Ciudad Autónoma de Buenos Aires, La Plata, Resistencia, and Misión Nueva Pompeya), calculated the population parameters, and evaluated the possible association among pain sensitivity, clinical, and genetic variables through a Generalised Estimating Equation model. High linkage disequilibrium was observed in the four populations for both genes, and significant differences were found among frequencies of Argentinian populations and those from other continents reported in the 1000 Genomes Project. Four PDYN gene polymorphisms from 3´ untranslated region and exon 4 showed association with acute pain sensitivity. One genotype of each of these polymorphisms was associated with a higher pain sensitivity, probably related with the activation of the N-methyl-D-aspartate (NMDA) receptors. We found a strong association with acute pain for the following clinical variables: 1) time after surgery, 2) intravenous klosidol supplied every 8 h, and 3) type of incision. Our results highlight the importance of a regional study of genetic variants which influence pain sensitivity and analgesic response. |
| format |
Articulo Articulo |
| author |
Di Santo Meztler, Gabriela Paula Schiaffi, Jorge Rigalli, Alfredo Esteban Torné, María Esther Martina, Pablo Francisco Catanesi, Cecilia Inés |
| author_facet |
Di Santo Meztler, Gabriela Paula Schiaffi, Jorge Rigalli, Alfredo Esteban Torné, María Esther Martina, Pablo Francisco Catanesi, Cecilia Inés |
| author_sort |
Di Santo Meztler, Gabriela Paula |
| title |
Variability of PDYN and OPRK1 genes in four Argentinian populations and its genetic association with clinical variables related to acute postsurgical pain |
| title_short |
Variability of PDYN and OPRK1 genes in four Argentinian populations and its genetic association with clinical variables related to acute postsurgical pain |
| title_full |
Variability of PDYN and OPRK1 genes in four Argentinian populations and its genetic association with clinical variables related to acute postsurgical pain |
| title_fullStr |
Variability of PDYN and OPRK1 genes in four Argentinian populations and its genetic association with clinical variables related to acute postsurgical pain |
| title_full_unstemmed |
Variability of PDYN and OPRK1 genes in four Argentinian populations and its genetic association with clinical variables related to acute postsurgical pain |
| title_sort |
variability of pdyn and oprk1 genes in four argentinian populations and its genetic association with clinical variables related to acute postsurgical pain |
| publishDate |
2022 |
| url |
http://sedici.unlp.edu.ar/handle/10915/154324 |
| work_keys_str_mv |
AT disantomeztlergabrielapaula variabilityofpdynandoprk1genesinfourargentinianpopulationsanditsgeneticassociationwithclinicalvariablesrelatedtoacutepostsurgicalpain AT schiaffijorge variabilityofpdynandoprk1genesinfourargentinianpopulationsanditsgeneticassociationwithclinicalvariablesrelatedtoacutepostsurgicalpain AT rigallialfredo variabilityofpdynandoprk1genesinfourargentinianpopulationsanditsgeneticassociationwithclinicalvariablesrelatedtoacutepostsurgicalpain AT estebantornemariaesther variabilityofpdynandoprk1genesinfourargentinianpopulationsanditsgeneticassociationwithclinicalvariablesrelatedtoacutepostsurgicalpain AT martinapablofrancisco variabilityofpdynandoprk1genesinfourargentinianpopulationsanditsgeneticassociationwithclinicalvariablesrelatedtoacutepostsurgicalpain AT catanesiceciliaines variabilityofpdynandoprk1genesinfourargentinianpopulationsanditsgeneticassociationwithclinicalvariablesrelatedtoacutepostsurgicalpain AT disantomeztlergabrielapaula variabilidaddelosgenespdynyoprk1encuatropoblacionesargentinasysuasociacionconvariablesclinicasrelacionadasaldoloragudopostquirurgico AT schiaffijorge variabilidaddelosgenespdynyoprk1encuatropoblacionesargentinasysuasociacionconvariablesclinicasrelacionadasaldoloragudopostquirurgico AT rigallialfredo variabilidaddelosgenespdynyoprk1encuatropoblacionesargentinasysuasociacionconvariablesclinicasrelacionadasaldoloragudopostquirurgico AT estebantornemariaesther variabilidaddelosgenespdynyoprk1encuatropoblacionesargentinasysuasociacionconvariablesclinicasrelacionadasaldoloragudopostquirurgico AT martinapablofrancisco variabilidaddelosgenespdynyoprk1encuatropoblacionesargentinasysuasociacionconvariablesclinicasrelacionadasaldoloragudopostquirurgico AT catanesiceciliaines variabilidaddelosgenespdynyoprk1encuatropoblacionesargentinasysuasociacionconvariablesclinicasrelacionadasaldoloragudopostquirurgico |
| _version_ |
1768902314730455040 |
| spelling |
I19-R120-10915-1543242023-06-14T20:28:45Z http://sedici.unlp.edu.ar/handle/10915/154324 issn:1852-6233 Variability of PDYN and OPRK1 genes in four Argentinian populations and its genetic association with clinical variables related to acute postsurgical pain Variabilidad de los genes PDYN y OPRK1 en cuatro poblaciones argentinas y su asociación con variables clínicas relacionadas al dolor agudo post-quirúrgico Di Santo Meztler, Gabriela Paula Schiaffi, Jorge Rigalli, Alfredo Esteban Torné, María Esther Martina, Pablo Francisco Catanesi, Cecilia Inés 2022-12 2023-06-14T17:33:12Z en Ciencias Exactas Biología human populations pain sensitivity acute pain genetic polymorphisms genetic structure poblaciones humanas sensibilidad al dolor dolor agudo polimorfismos genéticos estructura genética Several population studies showed an association between variation in pain sensitivity and genetic polymorphisms located in Prodynorphin (PDYN) and Kappa Opioid Receptor (OPRK1) human genes. We analysed polymorphisms of these two genes to characterise their variation in Argentinian populations, as well as to evaluate their association with acute pain sensitivity. We studied 11 genetic markers in individuals from four locations in Argentina (Ciudad Autónoma de Buenos Aires, La Plata, Resistencia, and Misión Nueva Pompeya), calculated the population parameters, and evaluated the possible association among pain sensitivity, clinical, and genetic variables through a Generalised Estimating Equation model. High linkage disequilibrium was observed in the four populations for both genes, and significant differences were found among frequencies of Argentinian populations and those from other continents reported in the 1000 Genomes Project. Four PDYN gene polymorphisms from 3´ untranslated region and exon 4 showed association with acute pain sensitivity. One genotype of each of these polymorphisms was associated with a higher pain sensitivity, probably related with the activation of the N-methyl-D-aspartate (NMDA) receptors. We found a strong association with acute pain for the following clinical variables: 1) time after surgery, 2) intravenous klosidol supplied every 8 h, and 3) type of incision. Our results highlight the importance of a regional study of genetic variants which influence pain sensitivity and analgesic response. La asociación entre la sensibilidad al dolor y los polimorfismos que presentan los genes humanos de prodinorfina (PDYN) y receptor opioide kappa (OPRK1) se ha evidenciado en distintos estudios poblacionales. Con el objetivo de caracterizar la variación de estos genes y evaluar su asociación con dolor agudo en la población argentina, analizamos 11 polimorfismos en individuos provenientes de cuatro localidades argentinas (Ciudad Autónoma de Buenos Aires, La Plata, Resistencia, y Misión Nueva Pompeya). Calculamos los parámetros poblacionales y evaluamos la posible asociación entre sensibilidad al dolor, variables clínicas y variables genéticas a través de un modelo de ecuación generalizada de estimación. Se observó alto desequilibrio de ligamiento para ambos genes en las cuatro poblaciones analizadas, y se encontraron diferencias significativas entre las frecuencias de poblaciones argentinas y las reportadas en el Proyecto 1000 Genomes para poblaciones de otros continentes. Cuatro polimorfismos de la región 3´UTR y el exón 4 de PDYN mostraron asociación con la sensibilidad al dolor agudo. En cada uno de estos polimorfismos, un genotipo resultó asociado con alta sensibilidad al dolor, probablemente en relación con la activación de receptores N-metil-D-aspartato (NMDA). Encontramos una fuerte asociación con dolor agudo para las siguientes variables clínicas: 1) tiempo post-cirugía, 2) administración intravenosa de klosidol cada 8 h, y 3) tipo de incisión. Nuestros resultados resaltan la importancia de realizar estudios regionales de variables genéticas que influyen en la sensibilidad al dolor y la respuesta analgésica. Centro de Investigación de Proteínas Vegetales Instituto Multidisciplinario de Biología Celular Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 7-18 |