Nanostructured Lipid Carriers Loaded with Dexamethasone Prevent Inflammatory Responses in Primary Non-Parenchymal Liver Cells

Liver inflammation represents a major clinical problem in a wide range of pathologies. Among the strategies to prevent liver failure, dexamethasone (DXM) has been widely used to suppress inflammatory responses. The use of nanocarriers for encapsulation and sustained release of glucocorticoids to liv...

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Autores principales: Medina-Montano, Carolina, Rivero Berti, Ignacio, Gambaro, Rocío Celeste, Limeres, María José, Svensson, Malin, Padula, Gisel, Chain, Cecilia Yamil, Cisneros, José Sebastián, Castro, Guillermo Raúl, Grabbe, Stephan, Bros, Matthias, Gehring, Stephan, Islan, Germán Abel, Cacicedo, Maximiliano Luis
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Ciencias Exactas
Farmacia
glucocorticoids
lipid nanoparticles
liver inflammation
liver immunology
autoimmune hepatitis
dexamethasone
drug-controlled release
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/154307
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-154307
record_format dspace
spelling I19-R120-10915-1543072023-06-14T20:29:00Z http://sedici.unlp.edu.ar/handle/10915/154307 issn:1999-4923 Nanostructured Lipid Carriers Loaded with Dexamethasone Prevent Inflammatory Responses in Primary Non-Parenchymal Liver Cells Medina-Montano, Carolina Rivero Berti, Ignacio Gambaro, Rocío Celeste Limeres, María José Svensson, Malin Padula, Gisel Chain, Cecilia Yamil Cisneros, José Sebastián Castro, Guillermo Raúl Grabbe, Stephan Bros, Matthias Gehring, Stephan Islan, Germán Abel Cacicedo, Maximiliano Luis 2022-08 2023-06-14T14:55:13Z en Ciencias Exactas Farmacia glucocorticoids lipid nanoparticles liver inflammation liver immunology autoimmune hepatitis dexamethasone drug-controlled release Liver inflammation represents a major clinical problem in a wide range of pathologies. Among the strategies to prevent liver failure, dexamethasone (DXM) has been widely used to suppress inflammatory responses. The use of nanocarriers for encapsulation and sustained release of glucocorticoids to liver cells could provide a solution to prevent severe side effects associated with systemic delivery as the conventional treatment regime. Here we describe a nanostructured lipid carrier developed to efficiently encapsulate and release DXM. This nano-formulation proved to be stable over time, did not interact in vitro with plasma opsonins, and was well tolerated by primary non-parenchymal liver cells (NPCs). Released DXM preserved its pharmacological activity, as evidenced by inducing robust anti-inflammatory responses in NPCs. Taken together, nanostructured lipid carriers may constitute a reliable platform for the delivery of DXM to treat pathologies associated with chronic liver inflammation. Centro de Investigación y Desarrollo en Fermentaciones Industriales Instituto de Genética Veterinaria Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Farmacia
glucocorticoids
lipid nanoparticles
liver inflammation
liver immunology
autoimmune hepatitis
dexamethasone
drug-controlled release
spellingShingle Ciencias Exactas
Farmacia
glucocorticoids
lipid nanoparticles
liver inflammation
liver immunology
autoimmune hepatitis
dexamethasone
drug-controlled release
Medina-Montano, Carolina
Rivero Berti, Ignacio
Gambaro, Rocío Celeste
Limeres, María José
Svensson, Malin
Padula, Gisel
Chain, Cecilia Yamil
Cisneros, José Sebastián
Castro, Guillermo Raúl
Grabbe, Stephan
Bros, Matthias
Gehring, Stephan
Islan, Germán Abel
Cacicedo, Maximiliano Luis
Nanostructured Lipid Carriers Loaded with Dexamethasone Prevent Inflammatory Responses in Primary Non-Parenchymal Liver Cells
topic_facet Ciencias Exactas
Farmacia
glucocorticoids
lipid nanoparticles
liver inflammation
liver immunology
autoimmune hepatitis
dexamethasone
drug-controlled release
description Liver inflammation represents a major clinical problem in a wide range of pathologies. Among the strategies to prevent liver failure, dexamethasone (DXM) has been widely used to suppress inflammatory responses. The use of nanocarriers for encapsulation and sustained release of glucocorticoids to liver cells could provide a solution to prevent severe side effects associated with systemic delivery as the conventional treatment regime. Here we describe a nanostructured lipid carrier developed to efficiently encapsulate and release DXM. This nano-formulation proved to be stable over time, did not interact in vitro with plasma opsonins, and was well tolerated by primary non-parenchymal liver cells (NPCs). Released DXM preserved its pharmacological activity, as evidenced by inducing robust anti-inflammatory responses in NPCs. Taken together, nanostructured lipid carriers may constitute a reliable platform for the delivery of DXM to treat pathologies associated with chronic liver inflammation.
format Articulo
Articulo
author Medina-Montano, Carolina
Rivero Berti, Ignacio
Gambaro, Rocío Celeste
Limeres, María José
Svensson, Malin
Padula, Gisel
Chain, Cecilia Yamil
Cisneros, José Sebastián
Castro, Guillermo Raúl
Grabbe, Stephan
Bros, Matthias
Gehring, Stephan
Islan, Germán Abel
Cacicedo, Maximiliano Luis
author_facet Medina-Montano, Carolina
Rivero Berti, Ignacio
Gambaro, Rocío Celeste
Limeres, María José
Svensson, Malin
Padula, Gisel
Chain, Cecilia Yamil
Cisneros, José Sebastián
Castro, Guillermo Raúl
Grabbe, Stephan
Bros, Matthias
Gehring, Stephan
Islan, Germán Abel
Cacicedo, Maximiliano Luis
author_sort Medina-Montano, Carolina
title Nanostructured Lipid Carriers Loaded with Dexamethasone Prevent Inflammatory Responses in Primary Non-Parenchymal Liver Cells
title_short Nanostructured Lipid Carriers Loaded with Dexamethasone Prevent Inflammatory Responses in Primary Non-Parenchymal Liver Cells
title_full Nanostructured Lipid Carriers Loaded with Dexamethasone Prevent Inflammatory Responses in Primary Non-Parenchymal Liver Cells
title_fullStr Nanostructured Lipid Carriers Loaded with Dexamethasone Prevent Inflammatory Responses in Primary Non-Parenchymal Liver Cells
title_full_unstemmed Nanostructured Lipid Carriers Loaded with Dexamethasone Prevent Inflammatory Responses in Primary Non-Parenchymal Liver Cells
title_sort nanostructured lipid carriers loaded with dexamethasone prevent inflammatory responses in primary non-parenchymal liver cells
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/154307
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