Are integrins and ligands correlated at pig placental interface during pregnancy?

In the present work, we emphasize the studies about integrins and their receptors in pig placental interface at different times of gestation. Uterine placental interface (n = 24) of 17, 30, 60 and 70 days of gestation (dg) and non-pregnant uterus (n = 4) of crossbred sows were used. The presence of...

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Autores principales: Vélez, Carolina Lucía, Clauzure, Mariangeles, Williamson, Delia, Garcia, Monica, Koncurat, Mirta Adriana, Barbeito, Claudio Gustavo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Ciencias Veterinarias
Integrins
Fibronectin
Osteopontin
Pig gestation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/153825
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SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-153825
record_format dspace
spelling I19-R120-10915-1538252023-06-01T20:08:41Z http://sedici.unlp.edu.ar/handle/10915/153825 issn:2633-8386 Are integrins and ligands correlated at pig placental interface during pregnancy? Vélez, Carolina Lucía Clauzure, Mariangeles Williamson, Delia Garcia, Monica Koncurat, Mirta Adriana Barbeito, Claudio Gustavo 2023 2023-06-01T16:57:40Z en Ciencias Veterinarias Integrins Fibronectin Osteopontin Pig gestation In the present work, we emphasize the studies about integrins and their receptors in pig placental interface at different times of gestation. Uterine placental interface (n = 24) of 17, 30, 60 and 70 days of gestation (dg) and non-pregnant uterus (n = 4) of crossbred sows were used. The presence of αvβ3 (ITGAV (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) and α5β1 (ITGA5 (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) integrins, and their ligands fibronectin (FN) and osteopontin (OPN/ SPP1), was detected by immunohistochemistry, and the immunolabeled area percentage (IAP) and the optical density (OD) were determined. The integrins and its ligands analyzed have presented peaks of expression in early and mid-gestation, both in IAP and in the OD area, decreasing at 70 dg. These temporal changes showed us that the molecules studied in this work participate in embryo/feto–maternal attachment, variably. Besides, we found a significant correlation both in the intensity and in the extension of immunostaining for trophoblastic FN and endometrial αvβ3, and trophoblastic OPN and endometrial α5β1, throughout the entire pig pregnancy. At late gestation, there is notable placental remodeling with subsequent removal or renewal of folds at the uterine–placental interface that results in the loss of focal adhesions. The decrease of the expression of some integrins and their ligands in late gestation, particularly at 70 dg, would demonstrate that there would be other adhesion molecules and other ligands that could be participating in the establishment of the maternal–fetal interface. Facultad de Ciencias Veterinarias Articulo Articulo http://creativecommons.org/licenses/by-nc-nd/4.0/ Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Integrins
Fibronectin
Osteopontin
Pig gestation
spellingShingle Ciencias Veterinarias
Integrins
Fibronectin
Osteopontin
Pig gestation
Vélez, Carolina Lucía
Clauzure, Mariangeles
Williamson, Delia
Garcia, Monica
Koncurat, Mirta Adriana
Barbeito, Claudio Gustavo
Are integrins and ligands correlated at pig placental interface during pregnancy?
topic_facet Ciencias Veterinarias
Integrins
Fibronectin
Osteopontin
Pig gestation
description In the present work, we emphasize the studies about integrins and their receptors in pig placental interface at different times of gestation. Uterine placental interface (n = 24) of 17, 30, 60 and 70 days of gestation (dg) and non-pregnant uterus (n = 4) of crossbred sows were used. The presence of αvβ3 (ITGAV (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) and α5β1 (ITGA5 (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) integrins, and their ligands fibronectin (FN) and osteopontin (OPN/ SPP1), was detected by immunohistochemistry, and the immunolabeled area percentage (IAP) and the optical density (OD) were determined. The integrins and its ligands analyzed have presented peaks of expression in early and mid-gestation, both in IAP and in the OD area, decreasing at 70 dg. These temporal changes showed us that the molecules studied in this work participate in embryo/feto–maternal attachment, variably. Besides, we found a significant correlation both in the intensity and in the extension of immunostaining for trophoblastic FN and endometrial αvβ3, and trophoblastic OPN and endometrial α5β1, throughout the entire pig pregnancy. At late gestation, there is notable placental remodeling with subsequent removal or renewal of folds at the uterine–placental interface that results in the loss of focal adhesions. The decrease of the expression of some integrins and their ligands in late gestation, particularly at 70 dg, would demonstrate that there would be other adhesion molecules and other ligands that could be participating in the establishment of the maternal–fetal interface.
format Articulo
Articulo
author Vélez, Carolina Lucía
Clauzure, Mariangeles
Williamson, Delia
Garcia, Monica
Koncurat, Mirta Adriana
Barbeito, Claudio Gustavo
author_facet Vélez, Carolina Lucía
Clauzure, Mariangeles
Williamson, Delia
Garcia, Monica
Koncurat, Mirta Adriana
Barbeito, Claudio Gustavo
author_sort Vélez, Carolina Lucía
title Are integrins and ligands correlated at pig placental interface during pregnancy?
title_short Are integrins and ligands correlated at pig placental interface during pregnancy?
title_full Are integrins and ligands correlated at pig placental interface during pregnancy?
title_fullStr Are integrins and ligands correlated at pig placental interface during pregnancy?
title_full_unstemmed Are integrins and ligands correlated at pig placental interface during pregnancy?
title_sort are integrins and ligands correlated at pig placental interface during pregnancy?
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/153825
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