Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data

Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with nonenveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In to...

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Detalles Bibliográficos
Autores principales: Debat, Humberto, García, María Laura, Bejerman, Nicolás
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Biología
Plant viruses
Ophiovirus
Virus taxonomy
Metatranscriptomics
Virus discovery
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/153023
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-153023
record_format dspace
spelling I19-R120-10915-1530232023-05-16T04:06:21Z http://sedici.unlp.edu.ar/handle/10915/153023 issn:1999-4915 Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data Debat, Humberto García, María Laura Bejerman, Nicolás 2023 2023-05-15T18:11:54Z en Biología Plant viruses Ophiovirus Virus taxonomy Metatranscriptomics Virus discovery Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with nonenveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus. Instituto de Biotecnología y Biología Molecular Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Plant viruses
Ophiovirus
Virus taxonomy
Metatranscriptomics
Virus discovery
spellingShingle Biología
Plant viruses
Ophiovirus
Virus taxonomy
Metatranscriptomics
Virus discovery
Debat, Humberto
García, María Laura
Bejerman, Nicolás
Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data
topic_facet Biología
Plant viruses
Ophiovirus
Virus taxonomy
Metatranscriptomics
Virus discovery
description Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with nonenveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus.
format Articulo
Articulo
author Debat, Humberto
García, María Laura
Bejerman, Nicolás
author_facet Debat, Humberto
García, María Laura
Bejerman, Nicolás
author_sort Debat, Humberto
title Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data
title_short Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data
title_full Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data
title_fullStr Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data
title_full_unstemmed Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data
title_sort expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/153023
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AT garciamarialaura expandingtherepertoireoftheplantinfectingophiovirusesthroughmetatranscriptomicsdata
AT bejermannicolas expandingtherepertoireoftheplantinfectingophiovirusesthroughmetatranscriptomicsdata
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