Effectiveness of several nutritional sources on the virulence of <i>Beauveria bassiana</i> s.l. CEP147 against the planthopper <i>Delphacodes kuscheli</i>

Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) is the main planthopper vector of Mal de R ıo Cuarto virus (MRCV), a Fijivirus that severely affects maize production in Argentina. The effect of several nutritional sources on the virulence of Beauveria bassiana s.l. CEP147 (Balsamo-Crivelli) Vui...

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Autores principales: Toledo, Andrea Vanesa, Brentassi, María Eugenia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Ciencias Agrarias
agricultural pests
biological control
entomopathogenic fungi
nutrition
virulence factors
Hemiptera
Delphacidae
Delphacodes kuscheli
Beauveria bassiana
Fijivirus
planthopper
maize
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/151449
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-151449
record_format dspace
spelling I19-R120-10915-1514492023-04-13T20:06:03Z http://sedici.unlp.edu.ar/handle/10915/151449 issn:1570-7458 Effectiveness of several nutritional sources on the virulence of <i>Beauveria bassiana</i> s.l. CEP147 against the planthopper <i>Delphacodes kuscheli</i> Toledo, Andrea Vanesa Brentassi, María Eugenia 2022-02 2023-04-13T18:27:57Z en Ciencias Agrarias agricultural pests biological control entomopathogenic fungi nutrition virulence factors Hemiptera Delphacidae Delphacodes kuscheli Beauveria bassiana Fijivirus planthopper maize Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) is the main planthopper vector of Mal de R ıo Cuarto virus (MRCV), a Fijivirus that severely affects maize production in Argentina. The effect of several nutritional sources on the virulence of Beauveria bassiana s.l. CEP147 (Balsamo-Crivelli) Vuillemin (Hypocreales: Cordycipitaceae) against this planthopper was evaluated with the aim to select some of them to be incorporated in future mass production studies. Ten agar-agar 2% media were used. Media were supplemented with 10 g of sucrose and 4% flour of either amaranth, chia, flax, oat bran, parboiled rice, poppy, quinoa, or wheat germ. Sabouraud dextrose agar with 1% yeast extract (SDAY) was used as control, and sucrose agar medium supplemented with 2% chitin was used as a possible inductor of enzymatic activity. We evaluated the effect of each medium on colony growth, conidial yield, viability, size, adherence, hydrophobicity, protease activity, cumulative mortality, and median survival time (MST). Principal component analysis indicated that conidial viability and adherence explained the most of the total variance, indicating that these variables can be used as indicators of fungal virulence. Medium supplemented with chia flour not only increased conidial viability (100%) and adherence (0.09 A550) with respect to the control medium, but also produced high D. kuscheli mortality (91.7%), with the lowest MST (5.2 days).We suggest that chia flour, which is rich in carbohydrates, proteins, vitamins, andminerals, might be incorporated as a nutritional supplement to solid substrates in order to increase the virulence of B. bassiana s.l. CEP147 against this and other pests. However, future studies are necessary to evaluate the effectiveness of this nutritional source on solid-state fermentation. Puede accederse a los datos utilizados para este artículo haciendo clic en "Documentos relacionados". Centro de Investigaciones en Fitopatología Facultad de Ciencias Naturales y Museo Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf 134-144
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
agricultural pests
biological control
entomopathogenic fungi
nutrition
virulence factors
Hemiptera
Delphacidae
Delphacodes kuscheli
Beauveria bassiana
Fijivirus
planthopper
maize
spellingShingle Ciencias Agrarias
agricultural pests
biological control
entomopathogenic fungi
nutrition
virulence factors
Hemiptera
Delphacidae
Delphacodes kuscheli
Beauveria bassiana
Fijivirus
planthopper
maize
Toledo, Andrea Vanesa
Brentassi, María Eugenia
Effectiveness of several nutritional sources on the virulence of <i>Beauveria bassiana</i> s.l. CEP147 against the planthopper <i>Delphacodes kuscheli</i>
topic_facet Ciencias Agrarias
agricultural pests
biological control
entomopathogenic fungi
nutrition
virulence factors
Hemiptera
Delphacidae
Delphacodes kuscheli
Beauveria bassiana
Fijivirus
planthopper
maize
description Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) is the main planthopper vector of Mal de R ıo Cuarto virus (MRCV), a Fijivirus that severely affects maize production in Argentina. The effect of several nutritional sources on the virulence of Beauveria bassiana s.l. CEP147 (Balsamo-Crivelli) Vuillemin (Hypocreales: Cordycipitaceae) against this planthopper was evaluated with the aim to select some of them to be incorporated in future mass production studies. Ten agar-agar 2% media were used. Media were supplemented with 10 g of sucrose and 4% flour of either amaranth, chia, flax, oat bran, parboiled rice, poppy, quinoa, or wheat germ. Sabouraud dextrose agar with 1% yeast extract (SDAY) was used as control, and sucrose agar medium supplemented with 2% chitin was used as a possible inductor of enzymatic activity. We evaluated the effect of each medium on colony growth, conidial yield, viability, size, adherence, hydrophobicity, protease activity, cumulative mortality, and median survival time (MST). Principal component analysis indicated that conidial viability and adherence explained the most of the total variance, indicating that these variables can be used as indicators of fungal virulence. Medium supplemented with chia flour not only increased conidial viability (100%) and adherence (0.09 A550) with respect to the control medium, but also produced high D. kuscheli mortality (91.7%), with the lowest MST (5.2 days).We suggest that chia flour, which is rich in carbohydrates, proteins, vitamins, andminerals, might be incorporated as a nutritional supplement to solid substrates in order to increase the virulence of B. bassiana s.l. CEP147 against this and other pests. However, future studies are necessary to evaluate the effectiveness of this nutritional source on solid-state fermentation.
format Articulo
Articulo
author Toledo, Andrea Vanesa
Brentassi, María Eugenia
author_facet Toledo, Andrea Vanesa
Brentassi, María Eugenia
author_sort Toledo, Andrea Vanesa
title Effectiveness of several nutritional sources on the virulence of <i>Beauveria bassiana</i> s.l. CEP147 against the planthopper <i>Delphacodes kuscheli</i>
title_short Effectiveness of several nutritional sources on the virulence of <i>Beauveria bassiana</i> s.l. CEP147 against the planthopper <i>Delphacodes kuscheli</i>
title_full Effectiveness of several nutritional sources on the virulence of <i>Beauveria bassiana</i> s.l. CEP147 against the planthopper <i>Delphacodes kuscheli</i>
title_fullStr Effectiveness of several nutritional sources on the virulence of <i>Beauveria bassiana</i> s.l. CEP147 against the planthopper <i>Delphacodes kuscheli</i>
title_full_unstemmed Effectiveness of several nutritional sources on the virulence of <i>Beauveria bassiana</i> s.l. CEP147 against the planthopper <i>Delphacodes kuscheli</i>
title_sort effectiveness of several nutritional sources on the virulence of <i>beauveria bassiana</i> s.l. cep147 against the planthopper <i>delphacodes kuscheli</i>
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/151449
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