Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina

Indirect and dot enzyme linked immunosorbent assays (ELISAs) were developed to detect antibodies to pseudorabies virus (PRV) in serum samples of 321 pigs. The Argentine CL 15 PRV strain was used as the source of virus. Nonidet P-40-solubilized infected and mock infected cell lysates were used as ant...

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Autores principales: Nosetto, Edgardo Omar, Echeverría, María Gabriela, Gimeno, Emilio Juan, Pecoraro, Marcelo Ricardo Ítalo, Etcheverrigaray, María Elisa
Formato: Objeto de conferencia
Lenguaje:Inglés
Publicado: 1990
Materias:
Ciencias Veterinarias
ELISA
Técnicas de laboratorio
Porcinos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/151289
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-151289
record_format dspace
spelling I19-R120-10915-1512892023-04-11T20:09:26Z http://sedici.unlp.edu.ar/handle/10915/151289 Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina Nosetto, Edgardo Omar Echeverría, María Gabriela Gimeno, Emilio Juan Pecoraro, Marcelo Ricardo Ítalo Etcheverrigaray, María Elisa 1990 1992-07 2023-04-11T14:35:17Z en Ciencias Veterinarias ELISA Técnicas de laboratorio Porcinos Indirect and dot enzyme linked immunosorbent assays (ELISAs) were developed to detect antibodies to pseudorabies virus (PRV) in serum samples of 321 pigs. The Argentine CL 15 PRV strain was used as the source of virus. Nonidet P-40-solubilized infected and mock infected cell lysates were used as antigens. The results of these tests were compared with those of a blocking ELISA and virus neutralization (VN) test. There was a 100% agreement between the indirect and dot-ELISAs. Using VN as standard, the blocking ELISA and the indirect ELISA showed 97.6% and 100% relative sensitivity and 96.9% and 99% relative specificity respectively. The sensitivity of the blocking ELISA was lower than that of the other tests. Facultad de Ciencias Veterinarias Objeto de conferencia Objeto de conferencia http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 59-64
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
ELISA
Técnicas de laboratorio
Porcinos
spellingShingle Ciencias Veterinarias
ELISA
Técnicas de laboratorio
Porcinos
Nosetto, Edgardo Omar
Echeverría, María Gabriela
Gimeno, Emilio Juan
Pecoraro, Marcelo Ricardo Ítalo
Etcheverrigaray, María Elisa
Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
topic_facet Ciencias Veterinarias
ELISA
Técnicas de laboratorio
Porcinos
description Indirect and dot enzyme linked immunosorbent assays (ELISAs) were developed to detect antibodies to pseudorabies virus (PRV) in serum samples of 321 pigs. The Argentine CL 15 PRV strain was used as the source of virus. Nonidet P-40-solubilized infected and mock infected cell lysates were used as antigens. The results of these tests were compared with those of a blocking ELISA and virus neutralization (VN) test. There was a 100% agreement between the indirect and dot-ELISAs. Using VN as standard, the blocking ELISA and the indirect ELISA showed 97.6% and 100% relative sensitivity and 96.9% and 99% relative specificity respectively. The sensitivity of the blocking ELISA was lower than that of the other tests.
format Objeto de conferencia
Objeto de conferencia
author Nosetto, Edgardo Omar
Echeverría, María Gabriela
Gimeno, Emilio Juan
Pecoraro, Marcelo Ricardo Ítalo
Etcheverrigaray, María Elisa
author_facet Nosetto, Edgardo Omar
Echeverría, María Gabriela
Gimeno, Emilio Juan
Pecoraro, Marcelo Ricardo Ítalo
Etcheverrigaray, María Elisa
author_sort Nosetto, Edgardo Omar
title Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
title_short Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
title_full Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
title_fullStr Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
title_full_unstemmed Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
title_sort development of an indirect and a dot elisa to detect antibodies to porcine pseudorabies virus in argentina
publishDate 1990
url http://sedici.unlp.edu.ar/handle/10915/151289
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