Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>

Entomopathogenic fungi such as Beauveria bassiana are extensively used for the control of insect pests worldwide. They infect mostly by adhesion to the insect surface and penetration through the cuticle. However, some insects, such as the red flour beetle Tribolium castaneum (Herbst), have evolved r...

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Autores principales: Davyt Colo, Belén, Girotti, Juan Roberto, González, Andrés, Pedrini, Nicolás
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Biología
chemical ecology
benzoquinones
gene expression
odorant-binding proteins
chemosensory proteins
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/149686
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-149686
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
chemical ecology
benzoquinones
gene expression
odorant-binding proteins
chemosensory proteins
spellingShingle Biología
chemical ecology
benzoquinones
gene expression
odorant-binding proteins
chemosensory proteins
Davyt Colo, Belén
Girotti, Juan Roberto
González, Andrés
Pedrini, Nicolás
Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
topic_facet Biología
chemical ecology
benzoquinones
gene expression
odorant-binding proteins
chemosensory proteins
description Entomopathogenic fungi such as Beauveria bassiana are extensively used for the control of insect pests worldwide. They infect mostly by adhesion to the insect surface and penetration through the cuticle. However, some insects, such as the red flour beetle Tribolium castaneum (Herbst), have evolved resistance by embedding their cuticle with antifungal compounds. Thus, they avoid fungal germination on the cuticle, which result in low susceptibility to entomopathogenic fungi. In adult T. castaneum, these antifungals are the well-known defensive compounds methyl-1,4- and ethyl-1,4- benzoquinone. In this study, we added B. bassiana conidia on the diet of adult beetles to study the effect of the entomopathogen on the secretion and detection of the beetle volatile blend containing both benzoquinones. The compounds were analyzed by solid phase microextraction coupled to gas chromatography–flame ionization detection, and were detected by electroantennography. In addition, we measured the expression level of four genes encoding for two odorant-binding proteins (OBP), one chemosensory protein (CSP), and one odorant receptor (OR) in both healthy and fungustreated insects. Significant alterations in the secretion of both benzoquinones, as well as in the perception of methyl-1,4-benzoquinone, were found in fungus-treated insects. TcOBP7D, TcOBP0A and TcCSP3A genes were down-regulated in insects fed conidia for 12 and 48 h, and the latter gene was up-regulated in 72 h samples. TcOR1 expression was not altered at the feeding times studied. We conclude that fungus-treated insects alter both secretion and perception of benzoquinones, but additional functional and genetic studies are needed to fully understand the effects of fungal infection on the insect chemical ecology.
format Articulo
Articulo
author Davyt Colo, Belén
Girotti, Juan Roberto
González, Andrés
Pedrini, Nicolás
author_facet Davyt Colo, Belén
Girotti, Juan Roberto
González, Andrés
Pedrini, Nicolás
author_sort Davyt Colo, Belén
title Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
title_short Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
title_full Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
title_fullStr Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
title_full_unstemmed Secretion and Detection of Defensive Compounds by the Red Flour Beetle <i>Tribolium castaneum</i> Interacting with the Insect Pathogenic Fungus <i>Beauveria bassiana</i>
title_sort secretion and detection of defensive compounds by the red flour beetle <i>tribolium castaneum</i> interacting with the insect pathogenic fungus <i>beauveria bassiana</i>
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/149686
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