Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats

The release of 45Ca induced by Ca2+ was studied in genetically hypertensive (SHR) and normotensive (WKY) rats using EGTA-skinned aortic strips. Strips preloaded with 45Ca (pCa 6.6) were desaturated at 5mM of EGTA. A slow component of the washout in aorta from SHRs exhibited higher Ca content and a l...

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Detalles Bibliográficos
Autores principales: Aiello, Ernesto Alejandro, Grassi de Gende, Ángela Ofelia
Formato: Articulo
Lenguaje:Inglés
Publicado: 1995
Materias:
Ciencias Médicas
Genetically hypertensive rats
EGTA-skinned aortic strips
Ca2+-induced
Ca2+ release
Sarcoplasmic reticulum
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/145232
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-145232
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Genetically hypertensive rats
EGTA-skinned aortic strips
Ca2+-induced
Ca2+ release
Sarcoplasmic reticulum
spellingShingle Ciencias Médicas
Genetically hypertensive rats
EGTA-skinned aortic strips
Ca2+-induced
Ca2+ release
Sarcoplasmic reticulum
Aiello, Ernesto Alejandro
Grassi de Gende, Ángela Ofelia
Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
topic_facet Ciencias Médicas
Genetically hypertensive rats
EGTA-skinned aortic strips
Ca2+-induced
Ca2+ release
Sarcoplasmic reticulum
description The release of 45Ca induced by Ca2+ was studied in genetically hypertensive (SHR) and normotensive (WKY) rats using EGTA-skinned aortic strips. Strips preloaded with 45Ca (pCa 6.6) were desaturated at 5mM of EGTA. A slow component of the washout in aorta from SHRs exhibited higher Ca content and a lower rate of Ca leak than that in aorta from WKY rats. This slow component was stimulated during washing with 0.03, 0.3, 1 or 10μM free Ca2+. The release of 45Ca induced by Ca2+ proceeded at similar rates in preparations of the two strains. Compared to WKY aortic strips the stimulated efflux of 45Ca was greater in SHR aortic strips due to the higher Ca content. About half of the release of 45Ca induced by 1μM free Ca2+ during the first 6 minutes of stimulation was blocked by 0.6mM of ryanodine or 50μM of ruthenium red, thus identifying the sarcoplasmic reticulum as a source of Ca release. The results suggest that this intracellular storage of Ca in aorta from genetically hypertensive rats is relevant for the generation of high levels of cytosolic Ca2+.
format Articulo
Articulo
author Aiello, Ernesto Alejandro
Grassi de Gende, Ángela Ofelia
author_facet Aiello, Ernesto Alejandro
Grassi de Gende, Ángela Ofelia
author_sort Aiello, Ernesto Alejandro
title Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
title_short Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
title_full Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
title_fullStr Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
title_full_unstemmed Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
title_sort calcium-induced calcium release in egta-skinned aortic strips from genetically hypertensive and normotensive rats
publishDate 1995
url http://sedici.unlp.edu.ar/handle/10915/145232
work_keys_str_mv AT aielloernestoalejandro calciuminducedcalciumreleaseinegtaskinnedaorticstripsfromgeneticallyhypertensiveandnormotensiverats
AT grassidegendeangelaofelia calciuminducedcalciumreleaseinegtaskinnedaorticstripsfromgeneticallyhypertensiveandnormotensiverats
bdutipo_str Repositorios
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