Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)

Dactylogyridae is overwhelmingly the most abundant and diverse taxon among monogeneans in continental waters of South America. Their small body size requires considerable sampling effort and training for collecting and identifying the worms from the gills, skin, nasal cavities, and other microhabita...

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Autores principales: Rossin, María Alejandra, De Francesco, Pablo Nicolás, Timi, Juan T.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Veterinarias
Monogenea
SDS treatment
Proteinase K treatment
Laser confocal images
Autofluorescence
Three-dimensional reconstruction
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/145223
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-145223
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Monogenea
SDS treatment
Proteinase K treatment
Laser confocal images
Autofluorescence
Three-dimensional reconstruction
spellingShingle Ciencias Veterinarias
Monogenea
SDS treatment
Proteinase K treatment
Laser confocal images
Autofluorescence
Three-dimensional reconstruction
Rossin, María Alejandra
De Francesco, Pablo Nicolás
Timi, Juan T.
Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)
topic_facet Ciencias Veterinarias
Monogenea
SDS treatment
Proteinase K treatment
Laser confocal images
Autofluorescence
Three-dimensional reconstruction
description Dactylogyridae is overwhelmingly the most abundant and diverse taxon among monogeneans in continental waters of South America. Their small body size requires considerable sampling effort and training for collecting and identifying the worms from the gills, skin, nasal cavities, and other microhabitats. Indeed, diagnostic characteristics as sclerites and male copulatory complex are generally less than 100-μm long and are essential for taxonomic description and identification of species. Here, a combination of simple and routine methods for three-dimensional morphological studies on hard structures is proposed for dactylogirids: SDS treatment for clarification of specimens and enzymatic digestion with proteinase K for freeing sclerotized structures, followed by laser confocal microscopy. This method is applicable to fresh or fixed specimens and does not require staining or dehydration. Indeed, stable autofluorescence emission is detectable at 500–530 nm for bars, anchors, and male copulatory complex when excited by argon laser. Advantages of this protocol over previous methodologies for taking laser confocal images are discussed. Open access software for image processing was used for three-dimensional reconstruction of sclerotized structures generating models and full 360° rotation videos.
format Articulo
Articulo
author Rossin, María Alejandra
De Francesco, Pablo Nicolás
Timi, Juan T.
author_facet Rossin, María Alejandra
De Francesco, Pablo Nicolás
Timi, Juan T.
author_sort Rossin, María Alejandra
title Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)
title_short Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)
title_full Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)
title_fullStr Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)
title_full_unstemmed Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)
title_sort three-dimensional morphology of rigid structures as a tool for taxonomic studies of dactylogyridae (monogenea)
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/145223
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bdutipo_str Repositorios
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