Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars

Lectins from two Phaseolus vulgaris L. cultivars were isolated and purified by salt fractionation, affinity chromatography and gel permeation chromatography. The cultivars used were: ‘alubia’, with a low-nodulating ability, and Bat 76 with a good symbiotic aptitude. Differences in properties of the...

Descripción completa

Detalles Bibliográficos
Autores principales: Brelles Mariño, Graciela, Boiardi, José Luis
Formato: Articulo
Lenguaje:Inglés
Publicado: 1992
Materias:
Biología
Electrophoretic pattern
Haemagglutinating activity
Lectins
Phaseolus vulgaris
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/143261
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-143261
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Electrophoretic pattern
Haemagglutinating activity
Lectins
Phaseolus vulgaris
spellingShingle Biología
Electrophoretic pattern
Haemagglutinating activity
Lectins
Phaseolus vulgaris
Brelles Mariño, Graciela
Boiardi, José Luis
Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars
topic_facet Biología
Electrophoretic pattern
Haemagglutinating activity
Lectins
Phaseolus vulgaris
description Lectins from two Phaseolus vulgaris L. cultivars were isolated and purified by salt fractionation, affinity chromatography and gel permeation chromatography. The cultivars used were: ‘alubia’, with a low-nodulating ability, and Bat 76 with a good symbiotic aptitude. Differences in properties of the two lectins were noted: ‘alubia’ lectin gave only one peak with haemagglutinating activity following gel permeation chromatography while Bat 76 yielded two active peaks, although both lectins had several bands of about 30 kDa following gel electrophoresis, Bat 76 lecting had three bands of about 50 kDa which were not present in ‘alubia’ and red kidney bean lectins. Peptide-mapping, by limited proteolysis and two dimensional gel electrophoresis, also showed differences between the lectins which are therefore judged to be different.
format Articulo
Articulo
author Brelles Mariño, Graciela
Boiardi, José Luis
author_facet Brelles Mariño, Graciela
Boiardi, José Luis
author_sort Brelles Mariño, Graciela
title Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars
title_short Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars
title_full Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars
title_fullStr Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars
title_full_unstemmed Role of bean lectins in Rhizobium phaseoli-Phaseolus vulgaris interactions: some properties of lectins from two bean cultivars
title_sort role of bean lectins in rhizobium phaseoli-phaseolus vulgaris interactions: some properties of lectins from two bean cultivars
publishDate 1992
url http://sedici.unlp.edu.ar/handle/10915/143261
work_keys_str_mv AT brellesmarinograciela roleofbeanlectinsinrhizobiumphaseoliphaseolusvulgarisinteractionssomepropertiesoflectinsfromtwobeancultivars
AT boiardijoseluis roleofbeanlectinsinrhizobiumphaseoliphaseolusvulgarisinteractionssomepropertiesoflectinsfromtwobeancultivars
bdutipo_str Repositorios
_version_ 1764820459784765441

Ejemplares similares