Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture

Outer membrane vesicles (OMV) derived from Bordetella pertussis—the etiologic agent of the resurgent disease called pertussis—are safe and effective in preventing bacterial colonization in the lungs of immunized mice. Vaccine formulations containing those OMV are capable of inducing a mixed Th1/Th2/...

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Autores principales: Carriquiriborde, Francisco Pablo, Aispuro, Pablo Martín, Ambrosis, Nicolás Martín, Zurita, María Eugenia, Bottero, Daniela, Gaillard, María Emilia, Castuma, Celina Elisabet, Rudi, Erika, Lodeiro, Aníbal Roberto, Hozbor, Daniela Flavia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Biología
Bordetella pertussis
outer membrane vesicles
biofilm
planktonic
protection
vaccine
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/143071
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-143071
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bordetella pertussis
outer membrane vesicles
biofilm
planktonic
protection
vaccine
spellingShingle Biología
Bordetella pertussis
outer membrane vesicles
biofilm
planktonic
protection
vaccine
Carriquiriborde, Francisco Pablo
Aispuro, Pablo Martín
Ambrosis, Nicolás Martín
Zurita, María Eugenia
Bottero, Daniela
Gaillard, María Emilia
Castuma, Celina Elisabet
Rudi, Erika
Lodeiro, Aníbal Roberto
Hozbor, Daniela Flavia
Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture
topic_facet Biología
Bordetella pertussis
outer membrane vesicles
biofilm
planktonic
protection
vaccine
description Outer membrane vesicles (OMV) derived from Bordetella pertussis—the etiologic agent of the resurgent disease called pertussis—are safe and effective in preventing bacterial colonization in the lungs of immunized mice. Vaccine formulations containing those OMV are capable of inducing a mixed Th1/Th2/Th17 profile, but even more interestingly, they may induce a tissue-resident memory immune response. This immune response is recommended for the new generation of pertussis-vaccines that must be developed to overcome the weaknesses of current commercial acellular vaccines (second-generation of pertussis vaccine). The third-generation of pertussis vaccine should also deal with infections caused by bacteria that currently circulate in the population and are phenotypically and genotypically different [in particular those deficient in the expression of pertactin antigen, PRN(-)] from those that circulated in the past. Here we evaluated the protective capacity of OMV derived from bacteria grown in biofilm, since it was observed that, by difference with older culture collection vaccine strains, circulating clinical B. pertussis isolates possess higher capacity for this lifestyle. Therefore, we performed studies with a clinical isolate with good biofilm-forming capacity. Biofilm lifestyle was confirmed by both scanning electron microscopy and proteomics. While scanning electron microscopy revealed typical biofilm structures in these cultures, BipA, fimbria, and other adhesins described as typical of the biofilm lifestyle were overexpressed in the biofilm culture in comparison with planktonic culture. OMV derived from biofilm (OMVbiof) or planktonic lifestyle (OMVplank) were used to formulate vaccines to compare their immunogenicity and protective capacities against infection with PRN(+) or PRN(-) B. pertussis clinical isolates. Using the mouse protection model, we detected that OMVbiof-vaccine was more immunogenic than OMVplank-vaccine in terms of both specific antibody titers and quality, since OMVbiof-vaccine induced antibodies with higher avidity. Moreover, when OMV were administered at suboptimal quantity for protection, OMVbiof-vaccine exhibited a significantly adequate and higher protective capacity against PRN(+) or PRN(-) than OMVplank-vaccine. Our findings indicate that the vaccine based on B. pertussis biofilm-derived OMV induces high protection also against pertactin-deficient strains, with a robust immune response.
format Articulo
Articulo
author Carriquiriborde, Francisco Pablo
Aispuro, Pablo Martín
Ambrosis, Nicolás Martín
Zurita, María Eugenia
Bottero, Daniela
Gaillard, María Emilia
Castuma, Celina Elisabet
Rudi, Erika
Lodeiro, Aníbal Roberto
Hozbor, Daniela Flavia
author_facet Carriquiriborde, Francisco Pablo
Aispuro, Pablo Martín
Ambrosis, Nicolás Martín
Zurita, María Eugenia
Bottero, Daniela
Gaillard, María Emilia
Castuma, Celina Elisabet
Rudi, Erika
Lodeiro, Aníbal Roberto
Hozbor, Daniela Flavia
author_sort Carriquiriborde, Francisco Pablo
title Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture
title_short Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture
title_full Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture
title_fullStr Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture
title_full_unstemmed Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture
title_sort pertussis vaccine candidate based on outer membrane vesicles derived from biofilm culture
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/143071
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