Counterbalance of Foxp3 and IDO expression at different tumor stages in aggressive breast cancer subtypes

Foxp3 and IDO1 are known immunomodulatory molecules involved in tumor escape and could be related to tumor infiltrating lymphocytes (TILs) in the tumor microenvironment. In this study, tumoral Foxp3 and IDO1 expression in breast cancer were evaluated in relation to lymphocyte biomarkers such as CD8...

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Detalles Bibliográficos
Autores principales: Canzoneri, Romina, Lacunza, Ezequiel, Rabassa, Martín Enrique, Cavalli, Fiorella A., Ferretti, Valeria Alejandra, Barbera, Luis A., Creton, Aldo, Croce, María Virginia, Isla Larrain, Marina Teresita
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2021
Materias:
Bioquímica
Ciencias Médicas
Foxp3
IDO1
TILs
Breast cancer
Tumor evasion
Immunomodulation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/142719
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-142719
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Ciencias Médicas
Foxp3
IDO1
TILs
Breast cancer
Tumor evasion
Immunomodulation
spellingShingle Bioquímica
Ciencias Médicas
Foxp3
IDO1
TILs
Breast cancer
Tumor evasion
Immunomodulation
Canzoneri, Romina
Lacunza, Ezequiel
Rabassa, Martín Enrique
Cavalli, Fiorella A.
Ferretti, Valeria Alejandra
Barbera, Luis A.
Creton, Aldo
Croce, María Virginia
Isla Larrain, Marina Teresita
Counterbalance of Foxp3 and IDO expression at different tumor stages in aggressive breast cancer subtypes
topic_facet Bioquímica
Ciencias Médicas
Foxp3
IDO1
TILs
Breast cancer
Tumor evasion
Immunomodulation
description Foxp3 and IDO1 are known immunomodulatory molecules involved in tumor escape and could be related to tumor infiltrating lymphocytes (TILs) in the tumor microenvironment. In this study, tumoral Foxp3 and IDO1 expression in breast cancer were evaluated in relation to lymphocyte biomarkers such as CD8 and CD45R0, regulatory T cells, as well as intratumoral and stromal TILs (iTILs and sTILs, respectively). Clinical and histopathological features were also included in the analysis. Foxp3 and IDO1 were found in tumor cells showing mainly cytoplasmic patterns in 60% and 62% tumor samples, respectively. TILs were found in 76% of samples; iTILs were detected in 92% of those samples and sTILs in 55%. Foxp3+ TILs were detected only in 12% of TILs+ samples associated with tumoral Foxp3 expression. Tumoral Foxp3 was mainly expressed at lower tumor stages while IDO1 expression was associated with advanced tumor stages; both correlated with CD8+ TILs which were observed in 77% of TILs+ samples. CD45R0+ were observed in 81% of TILs+ samples and correlated with higher tumor stages and poorly differentiated tumors. In ER negative tumors, an inverse correlation between Foxp3 and IDO1 tumoral expression was found in relation to tumor stage. TNBC subtype showed a positive correlation with the presence of iTILs. In silico analysis showed that Foxp3 and coexpressed genes in breast cancer were associated with immune response genes. Foxp3 was found predominantly in Basal and Her2-enriched subtypes in relation to Luminal A subtype, by RNA seq and RNA microarray database analysis. In conclusion, the expression of Foxp3 and IDO1 in tumors at different stages suggests a potential compensatory mechanism to evade the strong CTL response observed. This is relevant since the cumulative data indicates that Foxp3 as well as IDO1 could be potential targets of immunotherapy in patients with tumors at different stages and for the most aggressive breast cancer subtypes such as TNBC and Her2-enriched.
format Articulo
Preprint
author Canzoneri, Romina
Lacunza, Ezequiel
Rabassa, Martín Enrique
Cavalli, Fiorella A.
Ferretti, Valeria Alejandra
Barbera, Luis A.
Creton, Aldo
Croce, María Virginia
Isla Larrain, Marina Teresita
author_facet Canzoneri, Romina
Lacunza, Ezequiel
Rabassa, Martín Enrique
Cavalli, Fiorella A.
Ferretti, Valeria Alejandra
Barbera, Luis A.
Creton, Aldo
Croce, María Virginia
Isla Larrain, Marina Teresita
author_sort Canzoneri, Romina
title Counterbalance of Foxp3 and IDO expression at different tumor stages in aggressive breast cancer subtypes
title_short Counterbalance of Foxp3 and IDO expression at different tumor stages in aggressive breast cancer subtypes
title_full Counterbalance of Foxp3 and IDO expression at different tumor stages in aggressive breast cancer subtypes
title_fullStr Counterbalance of Foxp3 and IDO expression at different tumor stages in aggressive breast cancer subtypes
title_full_unstemmed Counterbalance of Foxp3 and IDO expression at different tumor stages in aggressive breast cancer subtypes
title_sort counterbalance of foxp3 and ido expression at different tumor stages in aggressive breast cancer subtypes
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/142719
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