Alternation between dietary protein depletion and normal feeding cause liver damage in mouse

The effect of frequent protein malnutrition on liver function has not been intensively examined. Thus, the effects of alternating 5 days of a protein and amino acid-free diet followed by 5 days of a complete diet repeated three times (3 PFD-CD) on female mouse liver were examined. The expression of...

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Autores principales: Caballero, Veronica Jorgelina, Mendieta, Julieta Renee, Giudici, Ana Marcela, Crupkin, Andrea Carina, Barbeito, Claudio Gustavo, Ronchi, Virginia Paola, Chisari, Andrea, Conde, Ruben Danilo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Veterinaria
Ciencias Exactas
Mouse liver
Cycles of protein depletion
Liver damage
Oxidative stress
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/142085
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-142085
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Ciencias Exactas
Mouse liver
Cycles of protein depletion
Liver damage
Oxidative stress
spellingShingle Veterinaria
Ciencias Exactas
Mouse liver
Cycles of protein depletion
Liver damage
Oxidative stress
Caballero, Veronica Jorgelina
Mendieta, Julieta Renee
Giudici, Ana Marcela
Crupkin, Andrea Carina
Barbeito, Claudio Gustavo
Ronchi, Virginia Paola
Chisari, Andrea
Conde, Ruben Danilo
Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
topic_facet Veterinaria
Ciencias Exactas
Mouse liver
Cycles of protein depletion
Liver damage
Oxidative stress
description The effect of frequent protein malnutrition on liver function has not been intensively examined. Thus, the effects of alternating 5 days of a protein and amino acid-free diet followed by 5 days of a complete diet repeated three times (3 PFD-CD) on female mouse liver were examined. The expression of carbonic anhydrase III (CAIII), fatty acid synthase (FAS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glutathione S-transferase P1 (GSTP1) in liver were assessed by proteomics, reverse transcriptase-polymerase chain reaction and Northern blotting. The activities of liver GSTs, glutathione reductase (GR) and catalase (CAT), as well as serum glutamic-oxaloacetic transaminase (SGOT) and glutamic-pyruvic transaminase (SGPT) were also tested. Additionally, oxidative damage was examined by measuring of protein carbonylation and lipid peroxidation. Liver histology was examined by light and electron microscopy. Compared with control mice, 3 PFD-CD increased the content of FAS protein (+90%) and FAS mRNA (+30%), while the levels of CAIII and CAIII mRNAs were decreased (-48% and -64%, respectively). In addition, 3 PFD-CD did not significantly change the content of GSTP1 but produced an increase in its mRNA level (+20%), while it decreased the activities of both CAT (-66%) and GSTs (-26%). After 3 PFD-CD, liver protein carbonylation and lipid peroxidation were increased by +55% and +95%, respectively. In serum, 3 PFD-CD increased the activities of both SGOT (+30%) and SGPT (+61%). In addition, 3 PFD-CD showed a histological pattern characteristic of hepatic damage. All together, these data suggest that frequent dietary amino acid deprivation causes hepatic metabolic and ultrastructural changes in a fashion similar to precancerous or cancerous conditions.
format Articulo
Articulo
author Caballero, Veronica Jorgelina
Mendieta, Julieta Renee
Giudici, Ana Marcela
Crupkin, Andrea Carina
Barbeito, Claudio Gustavo
Ronchi, Virginia Paola
Chisari, Andrea
Conde, Ruben Danilo
author_facet Caballero, Veronica Jorgelina
Mendieta, Julieta Renee
Giudici, Ana Marcela
Crupkin, Andrea Carina
Barbeito, Claudio Gustavo
Ronchi, Virginia Paola
Chisari, Andrea
Conde, Ruben Danilo
author_sort Caballero, Veronica Jorgelina
title Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
title_short Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
title_full Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
title_fullStr Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
title_full_unstemmed Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
title_sort alternation between dietary protein depletion and normal feeding cause liver damage in mouse
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/142085
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