Biosynthesis and degradation of glycerides in external mycelium of <i>Glomus mosseae</i>

The activities of enzymes involved in the glyceride metabolism of <i>Glomus mosseae</i> external mycelium are reported. Total mycelial homogenates were incubated with radiolabeled triolein and palmitic acid for various times under different conditions. The results obtained demonstrate th...

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Autores principales: Gaspar, María Laura, Pollero, Ricardo José, Cabello, Marta Noemí
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
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Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/141441
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id I19-R120-10915-141441
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Ciencias Naturales
Acyl-CoA ligase
Acylglycerol synthetase
External mycelium
Lipase activity
Triacylglycerols
spellingShingle Bioquímica
Ciencias Naturales
Acyl-CoA ligase
Acylglycerol synthetase
External mycelium
Lipase activity
Triacylglycerols
Gaspar, María Laura
Pollero, Ricardo José
Cabello, Marta Noemí
Biosynthesis and degradation of glycerides in external mycelium of <i>Glomus mosseae</i>
topic_facet Bioquímica
Ciencias Naturales
Acyl-CoA ligase
Acylglycerol synthetase
External mycelium
Lipase activity
Triacylglycerols
description The activities of enzymes involved in the glyceride metabolism of <i>Glomus mosseae</i> external mycelium are reported. Total mycelial homogenates were incubated with radiolabeled triolein and palmitic acid for various times under different conditions. The results obtained demonstrate the capacity of <i>G. mosseae</i> external mycelium to synthesize and hydrolyze its own acylglycerides. Neutral lipid biosynthesis progressively increased along with root colonization. Incorporation of [¹⁴C]-palmitate was mainly into triacylglycerols and as a minor fraction into diacylglycerols. The activity of palmitoyl-CoA ligase in external mycelium also increased in parallel with mycorrhiza development. The hydrolysis of triacylglycerols was very low at the beginning of colonization and then increased. However, lipase activity was lower than that of acyl-CoA ligase even at late stages of colonization. Thus, triacylglycerol biosynthesis apparently prevails over degradation during <i>G. mosseae</i> mycelium development in the period examined.
format Articulo
Articulo
author Gaspar, María Laura
Pollero, Ricardo José
Cabello, Marta Noemí
author_facet Gaspar, María Laura
Pollero, Ricardo José
Cabello, Marta Noemí
author_sort Gaspar, María Laura
title Biosynthesis and degradation of glycerides in external mycelium of <i>Glomus mosseae</i>
title_short Biosynthesis and degradation of glycerides in external mycelium of <i>Glomus mosseae</i>
title_full Biosynthesis and degradation of glycerides in external mycelium of <i>Glomus mosseae</i>
title_fullStr Biosynthesis and degradation of glycerides in external mycelium of <i>Glomus mosseae</i>
title_full_unstemmed Biosynthesis and degradation of glycerides in external mycelium of <i>Glomus mosseae</i>
title_sort biosynthesis and degradation of glycerides in external mycelium of <i>glomus mosseae</i>
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/141441
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