Isolation and analysis of endophytic microorganisms in wheat (<i>Triticum aestivum</i> L.) leaves

The present investigation was undertaken in order to select the surface-sterilization technique most efficient for eliminating epiphytes, to document the spectrum of endophytes of healthy leaves from three wheat cultivars in Buenos Aires Province (Argentina) and to determine their infection frequenc...

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Detalles Bibliográficos
Autores principales: Larrán, Silvina, Perelló, Analía Edith, Simón, María Rosa, Moreno, María Virginia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2002
Materias:
Ciencias Agrarias
Ciencias Naturales
endophytes
symptomless wheat leaves
Triticum aestivum
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/141438
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-141438
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Ciencias Naturales
endophytes
symptomless wheat leaves
Triticum aestivum
spellingShingle Ciencias Agrarias
Ciencias Naturales
endophytes
symptomless wheat leaves
Triticum aestivum
Larrán, Silvina
Perelló, Analía Edith
Simón, María Rosa
Moreno, María Virginia
Isolation and analysis of endophytic microorganisms in wheat (<i>Triticum aestivum</i> L.) leaves
topic_facet Ciencias Agrarias
Ciencias Naturales
endophytes
symptomless wheat leaves
Triticum aestivum
description The present investigation was undertaken in order to select the surface-sterilization technique most efficient for eliminating epiphytes, to document the spectrum of endophytes of healthy leaves from three wheat cultivars in Buenos Aires Province (Argentina) and to determine their infection frequencies at three growth stages. Surface-sterilization with undiluted commercial solution of sodium hypochlorite was reaffirmed as adequate for removing epiphytes on wheat leaves. From the 450 wheat leaf segments incubated, three bacterial isolates and 130 fungal isolates were obtained. From all the isolates, 19 fungal species were identified. Bacterial isolates were characterized as <i>Bacillus</i> sp. There were significant differences between microorganisms, stages of growth, and stages × microorganisms interaction. Differences between cultivars, stages × cultivars, microorganisms × cultivars and for the triple interaction were not significant. Frequency of microorganisms isolated increased with crop age, but it was statistically similar for the three wheat cultivars tested (Klein Centauro, Klein Dragon and Buck Ombu). <i>Rhodotorula rubra</i>, <i>Alternaria alternata</i>, <i>Cladosporium herbarum</i> and <i>Epicoccum nigrum</i> were isolated in the highest frequency. The other microorganisms were present at intermediate or low values. The species isolated may be assigned to three groups: (a) well-known and economically important pathogens of wheat, (b) commonly abundant phylloplane fungi considered to be primary saprobic and minor pathogens and (c) species occasionally present in wheat.
format Articulo
Articulo
author Larrán, Silvina
Perelló, Analía Edith
Simón, María Rosa
Moreno, María Virginia
author_facet Larrán, Silvina
Perelló, Analía Edith
Simón, María Rosa
Moreno, María Virginia
author_sort Larrán, Silvina
title Isolation and analysis of endophytic microorganisms in wheat (<i>Triticum aestivum</i> L.) leaves
title_short Isolation and analysis of endophytic microorganisms in wheat (<i>Triticum aestivum</i> L.) leaves
title_full Isolation and analysis of endophytic microorganisms in wheat (<i>Triticum aestivum</i> L.) leaves
title_fullStr Isolation and analysis of endophytic microorganisms in wheat (<i>Triticum aestivum</i> L.) leaves
title_full_unstemmed Isolation and analysis of endophytic microorganisms in wheat (<i>Triticum aestivum</i> L.) leaves
title_sort isolation and analysis of endophytic microorganisms in wheat (<i>triticum aestivum</i> l.) leaves
publishDate 2002
url http://sedici.unlp.edu.ar/handle/10915/141438
work_keys_str_mv AT larransilvina isolationandanalysisofendophyticmicroorganismsinwheatitriticumaestivumilleaves
AT perelloanaliaedith isolationandanalysisofendophyticmicroorganismsinwheatitriticumaestivumilleaves
AT simonmariarosa isolationandanalysisofendophyticmicroorganismsinwheatitriticumaestivumilleaves
AT morenomariavirginia isolationandanalysisofendophyticmicroorganismsinwheatitriticumaestivumilleaves
bdutipo_str Repositorios
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