Gonadotrophin-releasing activity of histones H2A and H2B

We report that histones H2A and H2B possess gonadotrophin-releasing activity in vitro and assess the signal transduction pathways involved in these effects. Perifused and incubated rat anterior pituitary (AP) cells were used, and luteinizing hormone (LH) and follicle stimulating hormone (FSH) were m...

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Detalles Bibliográficos
Autores principales: Brown, Oscar Alfredo, Sosa, Yolanda Elena, Goya, Rodolfo Gustavo
Formato: Articulo
Lenguaje:Inglés
Publicado: 1998
Materias:
Medicina
Biología
Histones
pituitary cells
FSH
LH
MB35
calcium
diacylglycerol
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/141226
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-141226
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Biología
Histones
pituitary cells
FSH
LH
MB35
calcium
diacylglycerol
spellingShingle Medicina
Biología
Histones
pituitary cells
FSH
LH
MB35
calcium
diacylglycerol
Brown, Oscar Alfredo
Sosa, Yolanda Elena
Goya, Rodolfo Gustavo
Gonadotrophin-releasing activity of histones H2A and H2B
topic_facet Medicina
Biología
Histones
pituitary cells
FSH
LH
MB35
calcium
diacylglycerol
description We report that histones H2A and H2B possess gonadotrophin-releasing activity in vitro and assess the signal transduction pathways involved in these effects. Perifused and incubated rat anterior pituitary (AP) cells were used, and luteinizing hormone (LH) and follicle stimulating hormone (FSH) were measured by RIA. Perifusion of cells with histone H2A (30 muM) or histone H2B (30 muM), markedly stimulated LH release but failed to elicit any FSH response. Cells incubated with 6 or 30 muM histone H2A showed a dose- and time-dependent stimulatory effect on bot LH and FSH release which was blocked by 1 muM peptide MB35, an 86-120 amino acid fragment of histone H2A. Incubation of pituitary cells with gonadotrophin-releasing hormone (GnRH) and histones H2A or H2B showed a stimulatory effect on LH and FSH release which was similar to the sum of the separate effects. Trifluoperazine as well as ethylene glycol bis(b-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA), alone or in the presence of the calcium ionophore A23187, significantly reduced the response of AP cells to histones. Various cyclic adenosine monophosphate (cAMP) enhancers had no effect on histone-stimulated release of gonadotrophins in incubated AP cells. Our results confirm previous evidence that histones may act as hypophysiotrophic signals. Calcium- and diacylglycerol-associated pathways, but not cAMP, appear to participate in these effects.
format Articulo
Articulo
author Brown, Oscar Alfredo
Sosa, Yolanda Elena
Goya, Rodolfo Gustavo
author_facet Brown, Oscar Alfredo
Sosa, Yolanda Elena
Goya, Rodolfo Gustavo
author_sort Brown, Oscar Alfredo
title Gonadotrophin-releasing activity of histones H2A and H2B
title_short Gonadotrophin-releasing activity of histones H2A and H2B
title_full Gonadotrophin-releasing activity of histones H2A and H2B
title_fullStr Gonadotrophin-releasing activity of histones H2A and H2B
title_full_unstemmed Gonadotrophin-releasing activity of histones H2A and H2B
title_sort gonadotrophin-releasing activity of histones h2a and h2b
publishDate 1998
url http://sedici.unlp.edu.ar/handle/10915/141226
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AT sosayolandaelena gonadotrophinreleasingactivityofhistonesh2aandh2b
AT goyarodolfogustavo gonadotrophinreleasingactivityofhistonesh2aandh2b
bdutipo_str Repositorios
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